›› 2001, Vol. 21 ›› Issue (1): 29-32.

• 论文 • 上一篇    下一篇

提高人胃腺癌组织裸小鼠移植瘤成功率的几种方法选择

  

  1. 上海第二医科大学生化教研室分子生物学实验室,上海 200025
  • 出版日期:2001-01-25 发布日期:2013-03-19
  • 基金资助:

    国家“863”高技术项目(863-Z20-01-04)

Improving Tansplantation of Human Gastric Adenocarcinoma Tissue in Nude Mice

  1. Laboratory of Molecular Biology , Biochemistry Department, Shanghai Second Medical University ,Shanghai 200025,China
  • Online:2001-01-25 Published:2013-03-19

摘要: 采用2周龄强制断奶和3周龄自然断奶的2组裸鼠用皮下塞入法将手术切除的人肿瘤组织(肿瘤表面组织和深部组织)作异体移植,并将已长成的人肿瘤的异体瘤切除以残瘤的形式继续在裸鼠皮下增殖,观察移植瘤在不同周龄的裸鼠以及肿瘤组织取材差异的成瘤影响和残瘤的生长特性。结果表明,2周龄裸鼠移植瘤的成瘤性较3周龄裸鼠为优,在相同移植天数,2周龄的成瘤期比3周龄的裸鼠提前了约4d,成瘤率也提高了一倍以上(P<0.01)。移植时取肿瘤表面组织比肿瘤深部组织的成瘤率提高3倍以上(P<0.005)。残瘤的传代率100%,且成瘤期只有8d。结果提示,使用2周龄的裸小鼠可提高移植瘤的成瘤率,如接种时选择肿瘤表面组织更可提高移植瘤的成瘤率。在移植瘤成瘤后用残瘤的方式传代时,可使成瘤期提前。

关键词: 裸鼠, 移植瘤, 肿瘤组织, 成瘤率

Abstract: "The human gastric adcnocarcinoma tissues with or without superficial tissue were subcutancously transplanted into two weeks old ablactation nude micc or three weeks old weaning nude mice, then the formed heterogeneous tumor was excised and the remnant tumor continued to grow subcutancously in nude mice, the differences of the tumor formation rate of the implanted tumor as well as the growing characteristics of remnant tumor in nude mice of different ages, and the effects of tissue selection of human tumor were observed. The tumor formation of implanted tumor is superior in two weeks old to three weeks old mice. In the same period, the tumor formation in 2 weeks old was 4 days before than in 3 weeks old mice, with two times higher in the tu-mor formation rate. Tumor formation rale in group of tumor tissue with capsule was three times higher than that without capsule. The passage rate of the remnant tumor was 100%, and the tumor forming time is only 8 days. It can be concludcd that the tu-mor formation rate of implanted tumor can be improved by using two weeks old mice, it also can be much higher if using tumor tissues with superficial tissue. Tumor formation time can be shortened by using remnant tumor in passage after implanted tumor formed.
"

Key words: Nude mice, Implanted tumor, Tumor tissue. Tumor formalion rate