β-咔啉类衍生物对BALB/c裸小鼠肝癌皮下移植瘤生长的影响

doi:10.3969/j.issn.1674-5817.2013.04.009

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (4): 290-295.DOI: 10.3969/j.issn.1674-5817.2013.04.009

β-咔啉类衍生物对BALB/c裸小鼠肝癌皮下移植瘤生长的影响

张燕¹, 范文玺², 徐艺玫¹, 史深¹, 贵友军¹, 燕顺生¹

1.新疆维吾尔自治区实验动物研究中心, 乌鲁木齐 830002;
2.新疆华世丹药物研究有限责任公司, 乌鲁木齐 830000

收稿日期:2012-11-06 出版日期:2013-08-25 发布日期:2013-08-25
作者简介:张燕(1983-),女,实验师,从事实验动物与比较医学研究工作,E-mail:493396477@qq.com
基金资助:
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目(KY2012084)

Effect of β-carboline derivative on Subcutaneous Transplant Hepatocellular Carcinoma Growth in BALB/c Nude Mice

ZHANG Yan¹, FAN Wen-xi², XU Yi-mei¹, SHI Sheng¹, GUI You-jun¹, YAN Shun-sheng¹

1. Xinjiang Laboratory Animal Center, Ulumuq 830002, China;
2. Xinjiang HuaShiDan Pharmaceutical Research Co., LTD, Ulumuq 830000, China

Received:2012-11-06 Online:2013-08-25 Published:2013-08-25

摘要/Abstract

摘要： 目的观察β-咔啉类衍生物(HMD)对人肝癌裸小鼠皮下移植瘤生长的影响。方法雄性BALB/c裸小鼠每只以2×10⁷个活细胞/ml(0.2 ml)建立人肝癌的裸小鼠模型,随机分为HMD低剂量组(0.22 mg/kg); HMD中剂量组(0.44 mg/kg); HMD高剂量组(0.87 mg/kg); 5-氟尿嘧啶注射液组(5-Fu)和生理盐水对照组。各组连续给药15 d。观察HMD对裸小鼠人肝癌移植瘤生长的影响及对裸小鼠主要脏器的毒性。结果 HMD低、中、高剂量组及阳性组对裸小鼠人肝癌移植瘤体重的影响与阴性对照组比较均有极显著性差异(P<0.01)。HMD中、高剂量组及阳性组对裸小鼠体质量增加影响与阴性对照组比较有极显著性差异(P<0.01)。对肝脏重量及肝脏系数的影响,HMD低、中、高剂量组及阳性组与阴性对照组比较有显著性差异(P<0.05),肾脏及肾脏系数各组间差异不显著(P>0.05)。结论 HMD对人肝癌HepG2裸小鼠皮下移植瘤生长有明显的抑制作用,未见有异常毒性症状出现。

关键词: β-咔啉类衍生物(HMD), BALB/c裸小鼠, 人肝癌皮下移植瘤, 急性毒性实验

Abstract: Objective To investigate the effect of â-carboline derivative HMD on subcutaneous transplant hepatocellular carcinoma in BALB/c nude mice. Methods A tumor model of human HCC was created by subcutaneous inoculation of 2×10⁷ (cells/ml,0.2ml) HepG2 cells into BALB/c male nude mice. The mice were randomized to low-(0.22 mg/kg), medium-(0.44 mg/kg), and high-dose HMD(0.87 mg/kg)group, saline group and 5-Fu group. The mice in each group received iv injection of the drug for 15 days. The effects of HMD on the growth of human hepatocellular carcinoma, the weight and pathological changes of nude miceŁ§S main organs were observed. Results Compared with saline group, the mice in HMD groups and 5-Fu group showed significant inhibition in the growth of the transplanted tumor (P<0.01).The inhibitory rate of HMD on the mouse weight. Compared to the saline group, the inhibitory effects of HMD(0.44, 0.87 mg/kg) were of statistic significance (P<0.01). Contrast to the negative control, there were no significant difference to the weight of kidney and kidney index in HMD groups and 5-Fu group, but there were significant differeces in the weight of liver and liver index (P<0.05). Conclusion HMD exhibit an inhibitory effect on the growth of transplanted HepG2 tumor, and the mice don’t appear toxicity symptom.

Key words: β- carboline derivatives(HMD), BALB/c nude mice, Subcutaneous transplanted liver tumor, Acute toxicity

中图分类号:

Q95-33

张燕, 范文玺, 徐艺玫, 史深, 贵友军, 燕顺生. β-咔啉类衍生物对BALB/c裸小鼠肝癌皮下移植瘤生长的影响[J]. 实验动物与比较医学, 2013, 33(4): 290-295.

ZHANG Yan, FAN Wen-xi, XU Yi-mei, SHI Sheng, GUI You-jun, YAN Shun-sheng. Effect of β-carboline derivative on Subcutaneous Transplant Hepatocellular Carcinoma Growth in BALB/c Nude Mice[J]. Laboratory Animal and Comparative Medicine, 2013, 33(4): 290-295.

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β-咔啉类衍生物对BALB/c裸小鼠肝癌皮下移植瘤生长的影响

Effect of β-carboline derivative on Subcutaneous Transplant Hepatocellular Carcinoma Growth in BALB/c Nude Mice

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