薏苡仁油注射液对人体肝癌SMMC-7721裸鼠异种移植模型的抑制作用

doi:10.3969/j.issn.1674-5817.2012.02.011

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (2): 133-136.DOI: 10.3969/j.issn.1674-5817.2012.02.011

薏苡仁油注射液对人体肝癌SMMC-7721裸鼠异种移植模型的抑制作用

尹蓓珮, 陆宏祺, 沈龙海, 李炳生

上海医药工业研究院创新药物与制药工艺国家重点实验室, 上海 200437

收稿日期:2011-07-07 出版日期:2012-04-25 发布日期:2012-04-25
作者简介:尹蓓珮(1984-),女,药理学硕士,主要从事抗肿瘤药物临床前研究工作,E-mail:yinpeipei24@163.com
基金资助:
国家“重大新药创制”科技重大专项资助(项目编号: 2009ZX09301-007)

The Inhibiting Effect of Coix Seed Oil Injection on Xenograft Model of Human Liver Cancer Cell SMMC-7721

YIN Bei-pei, LU Hong-qi, SHEN Long-hai, LI Bing-sheng

State Key Laboratory of Innovative Medicines and Preparation Process, Shanghai Institute of Pharmaceutical Industry, Shanghai 200437, China

Received:2011-07-07 Online:2012-04-25 Published:2012-04-25

摘要/Abstract

摘要： 目的研究薏苡仁油注射液对裸鼠移植性人体肝癌SMMC-7721的抑制作用。方法建立人体肝癌SMMC-7721裸鼠异种移植模型, 通过尾静脉注射不同剂量的薏苡仁油注射液, 观察和测量肿瘤大小及荷瘤鼠体重, 计算相对肿瘤增殖率及肿瘤抑制率, 并对裸鼠肿瘤组织和主要脏器的石蜡切片分别进行Ki-67指标的免疫组化染色和HE染色。结果 25 ml/kg、12.5 ml/kg及6.25 ml/kg的薏苡仁油注射液对移植于裸鼠的人体肝癌SMMC-7721的相对肿瘤增殖率分别为33.02%、35.78%和39.41%,抑瘤率为49.54%、46.86%、41.71%。给药组裸鼠肿瘤组织的Ki-67阳性率明显低于对照组,主要脏器的HE染色未见异常。结论薏苡仁油注射液三个剂量组对移植于裸鼠的人体肝癌SMMC-7721模型都有一定的抑制作用,在体内均具有良好的抗肿瘤活性和安全性。

关键词: 薏苡仁油注射液, SMMC-7721, 裸鼠

Abstract: Objective To study the inhibiting effect of coix seed oil injection on human live cancer SMMC-7721 grafted in nude mice. Methods The model of human live cancer SMMC-7721 grafted in nude mice was established, the tumor size and body weight of mice bearing tumors were observed and measured, then relative tumor growth rates and tumor inhibition rates calculated. After the last treatment, tumors and various organs were harvested for immunohistochemistry and HE staining. Result The relative tumor growth rates of three dose groups of coix seed oil injection were 33.02%, 35.78% and 39.41%, and the inhibition rates were 49.54%, 46.86% and 41.71% respectively. The positive Ki-67 staining rate of tumors in nude mice treated with coix seed oil injection was significantly lower than that in the control group. Main organs showed normal state according to hematoxylin and eosin staining. Conclusion Coix seed oil injection has certain inhibition efficacy on the xenograft model of human liver cancer cell SMMC-7721.

Key words: Coix seed oil, SMMC-7721, Nude mice

中图分类号:

Q95-33

尹蓓珮, 陆宏祺, 沈龙海, 李炳生. 薏苡仁油注射液对人体肝癌SMMC-7721裸鼠异种移植模型的抑制作用[J]. 实验动物与比较医学, 2012, 32(2): 133-136.

YIN Bei-pei, LU Hong-qi, SHEN Long-hai, LI Bing-sheng. The Inhibiting Effect of Coix Seed Oil Injection on Xenograft Model of Human Liver Cancer Cell SMMC-7721[J]. Laboratory Animal and Comparative Medicine, 2012, 32(2): 133-136.

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薏苡仁油注射液对人体肝癌SMMC-7721裸鼠异种移植模型的抑制作用

The Inhibiting Effect of Coix Seed Oil Injection on Xenograft Model of Human Liver Cancer Cell SMMC-7721

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