参芪扶心口服液对睡眠剥夺大鼠利钾尿肽和心功能的影响

doi:10.3969/j.issn.1674-5817.2012.03.005

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (3): 195-198.DOI: 10.3969/j.issn.1674-5817.2012.03.005

参芪扶心口服液对睡眠剥夺大鼠利钾尿肽和心功能的影响

马路¹, 朱晓法¹, 钱彦方², 李秀玉², 赵美娜¹

中国人民解放军海军总医院 1.干部病房心血管内科;
2.中医科, 北京100048

收稿日期:2011-08-24 出版日期:2012-06-25 发布日期:2012-06-25
作者简介:马路(1960-), 男, 副主任医师, 从事老年心血管疾病的基础及临床研究,E-mail: maluzx2007@yahoo.com.cn
基金资助:
首都医学科学发展基金(SF-2009-Ⅲ-49)

Effects of Shenqifuxin Oral Liquid on Plasma Kaliuretic Peptide, Myocardial Contractility and Relaxation of Left Ventricle in Rats after Sleep Deprivation

MA Lu¹, ZHU Xiao-fa¹, QIAN Yan-fang², LI Xiu-yu², ZHAO Mei-na¹

1. Department of VIP Cardiology,
2. Department of Traditional Chinese Medicine,The Navy General Hospital of Chinese PLA, Beijing 100048, China

Received:2011-08-24 Online:2012-06-25 Published:2012-06-25

摘要/Abstract

摘要： 目的探讨参芪扶心口服液对睡眠剥夺(Sleep Deprivation, SD)大鼠心功能的影响。方法利用“小平台水环境法”(flower pot) 建立大鼠睡眠剥夺模型, 采用左心室插管术测定血液动力学指标; 放免法测定血浆利钾尿肽(KP)、心钠素(ANP)、血管紧张素Ⅱ(AngⅡ)及内皮素-1(ET-1)浓度。结果与模型组相比, 高剂量组等容收缩末期心肌缩短最大速度（Vmax）、左心室舒张期压力下降最大变化速率(-dp/dtmax)及左心室内压峰值(LVSP)增加, 左心室舒张末压（LVEDP）降低, 血浆 KP、AngⅡ及ET-1浓度降低, 均有显著性差异(P<0.05或P<0.01)。中、低剂量组血浆ANP水平下降明显(P均<0.01)。结论睡眠剥夺能够引起大鼠心功能障碍。参芪扶心口服液能降低睡眠剥夺引起心功能不全大鼠血浆KP、AngⅡ、ET-1浓度, 影响ANP分泌, 改善左室舒缩功能。

关键词: 睡眠剥夺, 参芪扶心口服液, 心功能, 利钾尿肽

Abstract: Objective To observe the effects of Shenqifuxin oral liquid (SQFXOL, 参芪扶心口服液) on plasma kaliuretic peptide (KP) and the left ventricular remodeling and the myocardial contractility and relaxation of left ventricle in rats after sleep deprivation. Methods The hemodynamic parameters were measured by the method of the catheterization. Plasma concentrations of KP, ANP, AngⅡ and ET-1 were determined by radioimmunoassays. Results In high dose group, the LVSP, dp/dtmax and Vmax were increased; LVEDP was decreased, plasma concentrations of KP, AngⅡ and ET-1 were decreased significantly(P<0.05 or P<0.01). The levels of plasma ANP in middle dose group and low dose group were significantly lower than those in model group (all P<0.01). Conclusion SQFXOL can reduce the plasma concentrations of KP, AngⅡ, ET-1, and ANP, improve the myocardial contractility and relaxation of left ventricular in rats with HF after myocardial infarction.

Key words: Shenqifuxin Oral Liquid, Sleep deprivation, Cardiac function, Kaliuretic peptide

中图分类号:

R338.8Q95-33

马路, 朱晓法, 钱彦方, 李秀玉, 赵美娜. 参芪扶心口服液对睡眠剥夺大鼠利钾尿肽和心功能的影响[J]. 实验动物与比较医学, 2012, 32(3): 195-198.

MA Lu, ZHU Xiao-fa, QIAN Yan-fang, LI Xiu-yu, ZHAO Mei-na. Effects of Shenqifuxin Oral Liquid on Plasma Kaliuretic Peptide, Myocardial Contractility and Relaxation of Left Ventricle in Rats after Sleep Deprivation[J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 195-198.

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参芪扶心口服液对睡眠剥夺大鼠利钾尿肽和心功能的影响

Effects of Shenqifuxin Oral Liquid on Plasma Kaliuretic Peptide, Myocardial Contractility and Relaxation of Left Ventricle in Rats after Sleep Deprivation

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