骨髓间充质干细胞对放射性回肠损伤的病理观察

doi:10.3969/j.issn.1674-5817.2012.03.004

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (3): 191-194.DOI: 10.3969/j.issn.1674-5817.2012.03.004

骨髓间充质干细胞对放射性回肠损伤的病理观察

宋祥福¹, 孙冰雪², 张静春³, 龚守良¹

1.吉林大学公共卫生学院卫生部放射生物学重点实验室, 长春130021;
2.吉林大学生命科学院药物制剂, 长春130021;
3.吉林大学中日联谊医院检验科, 长春 130031

收稿日期:2011-09-05 出版日期:2012-06-25 发布日期:2012-06-25
作者简介:宋祥福(1964-), 男, 高级工程师, 研究方向: MSCs与组织修复
基金资助:
吉林省科技厅项目资助课题(20080446)

Effect of Bone Marrow Stem Cells on Repairment of Ileum Radiation Damage

SONG Xiang-fu¹, SUN Bing-xue², ZHANG Jing-chun³, GONG Shou-liang¹

1. Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun 130021, China;
2. School of Life Sciences, Jilin University, Changchun 130021, China;
3. China-Japan Union Hospital of Jilin University, Changchun 130033, China

Received:2011-09-05 Online:2012-06-25 Published:2012-06-25

摘要/Abstract

摘要： 目的观察骨髓间充质干细胞(MSCs)在电离辐射诱发的空肠组织损伤中的迁徙、定居及修复作用。方法大鼠5 Gy X线全腹部照射,每隔72 h照射1次,共5次,制备放射性回肠损伤动物模型。大鼠随机分成正常对照组、模型组及MSCs治疗组,在激光共聚焦显微镜下观察MSCs在回肠组织中富集情况。病理学观察MSCs对放射性回肠损伤的修复作用。结果 MSCs经尾静脉注入2 d即富集回肠组织内,7 d后广泛分布在肠系膜血管周围。正常大鼠回肠黏膜结构清楚,模型组黏膜上皮细胞坏死脱落,以3 d较重,累及全层细胞变性; 治疗组的黏膜较相应的模型组厚,7 d肠黏膜坏死较模型组轻。结论 MSCs可以迅速地富集在放射性损伤的回肠组织中,促进回肠再生与修复。

关键词: 骨髓间充质干细胞, 电离辐射, 回肠, 组织修复

Abstract: Objective To observe the migration, colonization and repairing effects of marrow mesenchymal stem cells (MSCs) on ileum damage induced by radiation in rats. Methods The model of ileum radiation damage was made. Four rats from the ileum radiation damage model were sacrificed at 2 and 7d respectively after they were injected mesenchymal stem cells 1 ml (concentration:1 × 10⁶/ml), and ileal tissue were taken out, then the enrichment of MSCs in the ileum were observed under confocal microscope. Thirty-six rats were randomly divided into normal control group, model, and MSCs group (n=12). Animals in each group after the last injection of MSCs were sacrificed at 3 and 7 d, pathological changes of the ileum was observed. Results The occurrence of MSCs was observed in the ileum 2 d after MSC infusion, and widely distributed around the mesenteric vessels 7 d later. The intestinal structure was clear in normal control group, and epithelial cell was significantly necrosis at 3 d, involving the whole layer of cell degeneration in model group; compared with the model group, mucosal get thickness, and intestinal necrosis was relieved 7 d in treatment group. Conclusion The MSCs can be rapidly enriched in ileum radiation damage and promote regeneration and repairment.

Key words: Bone marrow mesenchymal stem cells, Ionizing radiation, Ileum, Tissue repair

中图分类号:

R329.2Q95-33

宋祥福, 孙冰雪, 张静春, 龚守良. 骨髓间充质干细胞对放射性回肠损伤的病理观察[J]. 实验动物与比较医学, 2012, 32(3): 191-194.

SONG Xiang-fu, SUN Bing-xue, ZHANG Jing-chun, GONG Shou-liang. Effect of Bone Marrow Stem Cells on Repairment of Ileum Radiation Damage[J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 191-194.

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骨髓间充质干细胞对放射性回肠损伤的病理观察

Effect of Bone Marrow Stem Cells on Repairment of Ileum Radiation Damage

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