人肺腺癌转移相关基因的初步筛选及验证

实验动物与比较医学 ›› 2010, Vol. 30 ›› Issue (5): 318-321.

人肺腺癌转移相关基因的初步筛选及验证

上海市肿瘤研究所实验病理研究室，上海，200032

出版日期:2010-05-31 发布日期:2010-05-31
基金资助:
国家973项目（编号：2009CB521803）；上海市科委创新行动计划项目（编号：08140902400）

Screening and Functional Analysis of Metastasis-associated Genes of Human Lung Cancer

Department of Experimental Pathology，Shanghai Cancer Institute, Shanghai 200032, China

Published:2010-05-31 Online:2010-05-31

摘要/Abstract

摘要： 目的利用建立的人肺癌高转移细胞系筛选转移基因，为研究肺癌转移的分子标志奠定基础。方法人肺癌细胞系SPC-A-1和相应的高转移细胞系SPC-A-1sci常规培养后提取总RNA，采用cDNA芯片技术检测两种细胞中差异表达基因，对部分有差异表达的基因进行蛋白免疫印迹（Western blot）分析验证。结果通过基因芯片技术筛选出了7 649个可能与肺癌转移相关的基因，其中上调基因3 891个，下调基因3 758个，功能涉及细胞黏附、凋亡、转录、信号传导、细胞周期和代谢等。经过Western分析，CD44与CDC42EP3在SPC-A-1sci中的表达明显高于SPC-A-1，而FNI的结果则相反。这与基因芯片的结果相一致。结论基因芯片高通量筛选提示7600余条基因可能与人肺癌转移相关，为进一步研究肺癌的转移机制和发现新的转移相关基因提供了较为有价值的线索。

Abstract: Objective To screen metastasis-associated genes by using highly metastatic animal and cell line for the purpose of the biomarker exploration of human lung cancer metastasis. Methods RNA from high metastatic SPC-A-1 sci cells and the low metastatic SPC-A-1 cells were isolated and purified，respectively. Differential profil-ing of gene expression between the two cells were screened by cDNA microarray. Western analyzed the expression of some differentally expressed genes. Results Total of 7649 genes, including 3891 genes up-regulated and 3758 genes down-regulated, were found to differentially express between the high metastatic and low metastatic cells by cDNA microarray. Those genes involved in adhesion, apoptosis, transcription, signal transduction, cell cycle and metabolism. After Western blot analysis, CD44 and CDC42EP3 in the SPC-A-1 sci expression was significantly higher than that of SPC-A-1, while the opposite result in FN1. This is consistent with the results of gene chips.Conclusions More than 7000 differential expressed genes might be associated with metastasis of human lung cancer. This study provided a promising reference for mechanism exploration of lung cancer metastasis and new metastatic related genes discovery.

Key words: Human lung cancer, Metastasis-associated genes, cDNA microarray, Western blot

刘蕾,王晓敏,荚德水,等. 人肺腺癌转移相关基因的初步筛选及验证[J]. 实验动物与比较医学, 2010, 30(5): 318-321.

LIU Lei,WANG Xiao-Min,JIA De-Shui,et al. Screening and Functional Analysis of Metastasis-associated Genes of Human Lung Cancer[J]. Laboratory Animal and Comparative Medicine, 2010, 30(5): 318-321.

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