人肺癌高转移细胞系的转移相关基因观察

doi:10.3969/j.issn.1674-5817.2011.02.001

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (2): 79-82.DOI: 10.3969/j.issn.1674-5817.2011.02.001

• 论著 • 下一篇

人肺癌高转移细胞系的转移相关基因观察

姚明^1,2, 李静², 荚德水², 张方琳¹, 闫明霞², 刘蕾², 张书霞¹

1.南京农业大学动物医学院, 南京 210095;
2.上海市肿瘤研究所实验病理研究室, 上海 200032

收稿日期:2010-12-20 出版日期:2011-04-25 发布日期:2011-04-25
作者简介:姚明(1959-), 男, 博士研究生,E-mail:myao@shsci.org
基金资助:
上海市科委创新行动计划(09140902000,10140902401)

Observation on Metastasis-related Genes of Highly Metastatic Human Lung Cancer Cell Line

YAO Ming^1,2, LI Jin², JIA De-shui², ZHANG Fan-lin¹, YAN Ming-xia², LIU Lei², ZHANG Shu-xia¹

1. Animal Medical College, NanJing Agriculture University, Nanjing, 210095, China;
2. Department of Experimental Pathology, Shanghai Cancer Institute, Shanghai 200032, China

Received:2010-12-20 Online:2011-04-25 Published:2011-04-25

摘要/Abstract

摘要： 目的观察人肺癌高转移细胞系SPC-A-1sci的转移相关基因表达,同时验证该细胞系的体内高转移特性。方法通过Western blot、FICC、IHC等方法检测转移相关基因CD44,OPN,MMP-9,EGFR在高、低转移细胞系间的差异表达;采用尾静脉注射方法观察高、低转移细胞系的转移特性。结果与低转移细胞系相比,转移促进基因CD44,OPN,MMP-9,EGFR在高转移细胞中的表达均显著上调。体内实验表明,8周时高转移细胞系的肺转移率为100%(12/12),而低转移细胞系的肺转移率仅为16.67%(2/12)。结论人肺癌高转移细胞系SPC-A-1sci在转移相关基因表达和动物体内均表现出典型的高转移特性,为该细胞系的推广应用提供了参考依据

关键词: 肺癌, 细胞株, 肿瘤转移, 转移相关基因

Abstract: Objective To observe the metastasis-associated genes expression in the highly metastatic human lung cancer cell line SPC-A-1sci and validate the highly metastatic characteristics of SPC-A-1sci in vivo. Methods The expression levels of several metastasis promoting genes concluding CD44, OPN, MMP-9, EGFR were analyzed between the high and low metastatic cell lines by RT-PCR, Western blotting, FICC, IHC and so on. The metastatic characteristics was investigated by injection the cell lines into the tail vein of nude mice. Results Compared with the low metastatic cell lines, the metastasis-associated genes CD44, OPN, MMP-9, EGFR in highly metastatic cells were significantly increased. After 8 weeks in vivo experiments, 100% (12/12) of mice injected with highly metastatic cell lines developed lung metastases, while low metastatic cell lines was 16.67%(2/12). Conclusions The metastasis-associated genes are highly expressed in SPC-A-1sci, and the SPC-A-1sci shows the typical characteristics of highly metastatic in vivo, and may be served as a reference for the application of the cell lines.

Key words: Human lung cancer, Cell lines, Cancer metastasis, Metastasis-associated genes

中图分类号:

R734.2Q95-33

姚明, 李静, 荚德水, 张方琳, 闫明霞, 刘蕾, 张书霞. 人肺癌高转移细胞系的转移相关基因观察[J]. 实验动物与比较医学, 2011, 31(2): 79-82.

YAO Ming, LI Jin, JIA De-shui, ZHANG Fan-lin, YAN Ming-xia, LIU Lei, ZHANG Shu-xia. Observation on Metastasis-related Genes of Highly Metastatic Human Lung Cancer Cell Line[J]. Laboratory Animal and Comparative Medicine, 2011, 31(2): 79-82.

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人肺癌高转移细胞系的转移相关基因观察

Observation on Metastasis-related Genes of Highly Metastatic Human Lung Cancer Cell Line

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