前肢畸形WHBE兔前肢骨密度检测及降钙素、骨钙素蛋白表达

doi:10.3969/j.issn.1674-5817.2015.05.008

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (5): 383-389.DOI: 10.3969/j.issn.1674-5817.2015.05.008

前肢畸形WHBE兔前肢骨密度检测及降钙素、骨钙素蛋白表达

陈方明, 林琳, 蔡月琴, 陈诚, 陈民利

浙江中医药大学动物实验研究中心, 杭州 310053

收稿日期:2014-12-11 出版日期:2015-10-25 发布日期:2015-10-25
作者简介:陈方明(1981-), 男, 实验师, 研究方向: 实验动物与比较医学。E-mail: 28483001@qq.com
基金资助:
1.浙江省科技厅公益性(动物平台)项目(2012C37079) 2.浙江省医药卫生科技计划项目(2008B137)

The Forelimb Bone Mineral Density and Expression of Calcitonin and Osteocalcin in Forelimb Malformed White Hair & Black Eyes Rabbits

CHEN Fang-ming, LIN Lin, CAI Yue-qin, CHEN Cheng, CHEN Min-li

The Research Center of Animal Experiment, Zhejiang Chinese Medicine University, Hangzhou 310053, China

Received:2014-12-11 Online:2015-10-25 Published:2015-10-25

摘要/Abstract

摘要： 目的观察前肢畸形的WHBE (FMWHBE) 兔前肢骨密度和降钙素(CT)、骨钙素(又称γ-羧基谷氨酸蛋白, BGP)蛋白表达水平,探讨前肢畸形的可能成因及作为骨生物力学研究的模型动物的可能性。方法分别选取1、2、3、4月龄FMWHBE兔、WHBE兔和日本大耳白兔,每个月龄阶段各6只。所有实验兔称体质量后耳动脉取血检测血清CT、BGP含量,用小动物成像系统测量前肢肱骨、桡骨、尺骨近心端、中段、远心端骨密度,并经脱钙后进行CT、BGP免疫组织化学染色观察。结果各组实验兔各月龄体质量无显著差异。FMWHBE兔肱骨1月龄全段和2月龄近心端、中段及3月龄肱骨远心端,桡骨1月龄中段和远心端及2、3月龄的远心端,尺骨1、2月龄全段及3月龄远心端骨密度均显著低于WHBE兔和日本大耳白兔组(P<0.05或P<0.01)。1月龄FMWHBE兔血清CT、BGP含量均显著降低(P<0.05或P<0.01),2月龄BGP显著高于日本大耳白兔(P<0.01),3月龄CT 显著高于WHBE兔(P<0.05)。CT、BGP免疫组织化学染色显示,FMWHBE兔CT表达阳性部位较少,阳性较弱,BGP表达稍弱于WHBE兔和日本大耳白兔。结论 FMWHBE兔发生前肢畸形和骨密度变化可能与其生长发育过程中趴卧姿态导致的前肢骨特殊应力及CT、BGP的表达特点有关。

关键词: 前肢畸形, WHBE兔, 骨密度, 降钙素, 骨钙素

Abstract: Objective To observe bone mineral density and expression level of calcitonin (CT), osteocalcin (also named bone gla protein, BGP) proteins in forelimb malformation WHBE rabbits (FMWHBE) and to explore possible cause of forelimb malformation and possibility to be an animal model for bone biomechanical studies. Method FMWHBE rabbits, WHBE rabbits and Japan white rabbits(JW) at 1, 2, 3 and 4 months of age were selected with six rabbits for each age. Contents of CT, BGP in serum were detected after body weighing and a small animal imaging system was used to measuring bone mineral density of proximal end, middle end and telecentric end in forelimb humerus, radius and ulna. CT and BGP immunohistochemical staining were used for pathological observation after decalcification. Result No significant difference of body weights of each month old was observed among all groups. Bone mineral density of whole length in 1 month old and proximal end, middle end in 2 months old and telecentric end in 3 months old of humerus, middle end and telecentric end in 1 month and telecentric end in 2 and 3 months old of radius and whole length in 1 and 2 months old and telecentric end in 3 months old of ulna in FMWHBE rabbits were notably lower than that in WHBE and JW rabbits (P<0.05 or P<0.01). CT and BGP contents in serum of 1 month old FMWHBE were notably decreased (P<0.05 or P<0.01). BGP level in 2 months old was obviously higher than that of in JW rabbits(P<0.01). CT level in 3 month olds was notably higher than that in WHBE rabbits (P<0.05). Immunohistochemical staining of CT, BGP showed that positive parts of CT expression in FMWHBE rabbit was less and weaker, and expression of BGP in FMWHBE rabbits was slightly weaker than that in JW rabbits and WHBE rabbits. Conclusion Forelimb malformation formation and bone mineral density changes in FMWHBE rabbits may be related with the special stress in forelimb bones and expression character of CT and BGP induced by lying posture during growth.

Key words: Forelimb malformed, WHBE rabbits, Bone mineral density, Calcitonin (CT), Osteocalcin (BGP)

中图分类号:

Q95-33

陈方明, 林琳, 蔡月琴, 陈诚, 陈民利. 前肢畸形WHBE兔前肢骨密度检测及降钙素、骨钙素蛋白表达[J]. 实验动物与比较医学, 2015, 35(5): 383-389.

CHEN Fang-ming, LIN Lin, CAI Yue-qin, CHEN Cheng, CHEN Min-li. The Forelimb Bone Mineral Density and Expression of Calcitonin and Osteocalcin in Forelimb Malformed White Hair & Black Eyes Rabbits[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 383-389.

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前肢畸形WHBE兔前肢骨密度检测及降钙素、骨钙素蛋白表达

The Forelimb Bone Mineral Density and Expression of Calcitonin and Osteocalcin in Forelimb Malformed White Hair & Black Eyes Rabbits

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