关键词: 5-脂氧化酶(5-LO), 动脉粥样硬化(AS), 转基因小鼠

Abstract: Objective To establishment a 5-lipoxygenase (5-LO) transgenic mouse model, which may be used for the research of the pathogenesis of atherosclerosis. Methods RT-PCR and immunohistichemistry was used to detect 5-LO transgenic mice and control mice. The cholesterol and blood pressure was also detected. Result Expression of 5-LO and 5-lipoxygenase activating protein (LFAP) were higher in the kidney and lung of the 5-LO transgenic mice than the control mice. The results of RT-PCR showed that the expression of leukotriene and its receptor were higher in the bone marrow cells, peritoneal cells, spleen and kidney of the 5-LO transgenic mice than the control mice. Compared the 5-LO transgenic mice with the control mice, there were significant differences in the expression of LTA4, LTB4 and Cys-LTR2 in the bone marrow cells and peritoneal cells (P<0.05), there were significant differences in the expression of LTB4, LTC4 and Cys-LTR2 in the spleen and kidney (P<0.05), there was a significant difference in the expression of Cys-LTR1 in the kidney (P<0.05), there were significant differences in the expression of CRP, IL-1b, PDGF and NF-kB in the kidney (P<0.05). The result of blood pressure determination showed that it was higher in the 5-LO transgenic mice than the control mice (P<0.05), and there was no significance in the cholesterol (P>0.05). Conclusion The 5-LO transgenic mouse model has been established.

Key words: 5-lipoxygenase(5-LO), Atherosclerosis, Transgenic mice