卵胞质内单精子注射与转座子介导的转基因技术

doi:10.3969/j.issn.1674-5817.2013.04.015

摘要/Abstract

摘要： 通过卵胞质内单精子注射(ICSI)已经成为治疗人类不育症和小鼠生殖生物学研究的一种有效手段。而且ICSI介导的转基因作为一种动物转基因技术,仍然不够完善。但是相比传统的原核注射技术,该技术最大的优势是可以导入较大片段的外源基因(比如人工酵母染色体YAC)。最近研究者们又发展了一种ICSI与转座子结合的转基因技术,能够高效地生产转基因后代,其效率几乎可以与慢病毒载体法相媲美,值得在转基因动物研究中加以推广和应用。

关键词: 卵胞质内单精子注射(ICSI), 转座子, 转基因

Abstract: Intracytoplasmic sperm injection (ICSI) has been widely used for generating offspring in human infertility clinics and in reproductive researched on mice. Many researchers engaged in animal transgenesis still consider it somewhat cumbersome. However, the greatest advantage of ICSI-mediated transgenesis is that it allows introduction of very large DNA transgenes (e.g., yeast artificial chromosomes), with relatively high efficiency into the genomes of hosts, as compared to pronuclear injection. Recently, researchers have developed an active form of intracytoplasmic sperm injection-mediated transgenesis (ICSI-Tr) with fresh sperm utilizing transposons. The transgenic efficiencies rival all transgenic techniques except that of lentiviral methods so that it is worthy of promotion and application in animal transgenic research.

Key words: ICSI, Transposon, Transgenesis

中图分类号:

Q95-33

孔彭成, 王美珊, 朱莲, 李和平, 蒋满喜, 陈学进. 卵胞质内单精子注射与转座子介导的转基因技术[J]. 实验动物与比较医学, 2013, 33(4): 319-323.

KONG Peng-cheng, WANG Mei-shan, ZHU Lian, LI He-ping, JIANG Man-xi, CHEN Xue-jin. Intracytoplasmic Sperm Injection and Transposon-mediated Transgenic Technology[J]. Laboratory Animal and Comparative Medicine, 2013, 33(4): 319-323.

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