Abstract: Objective To observe the effects of ozone major autohemotherapy on melatonin and oxidative stress in rats with sleep deprivation, and to provide theoretical basis for the treatment of ozone major autohemotherapy. Methods Forty adult male Wistar rats were selected as the study subjects. A rapid eye movement (REM) sleep deprivation model was subsequently developed using the “modified multi-platform sleep deprivation method”. Rats were randomly divided into the control group and the observation group according to the principle of random number table, with 20 rats in each group.The rats in the observation group were given autologous blood transfusion after ozone treatment, and the control rats were given air instead of ozone. Serum melatonin levels were measured at tail vein blood at 0 h, 24 h, 48 h and 72 h after intervention. The venous blood oxidative stress index [superoxide dismutase (SOD), glutathione peroxidase (GSH-px) and malondialdehyde (MDA)] levels were measured at 0 h and 72 h after intervention. Results There was no significant difference in wake (W), slow-wave sleep (SWS), and fast-wave sleep (FWS) time between the two groups of rats at the 0 h intervention (P>0.05). At 72 hours of intervention, W time in the observation group was shorter than that in the control group, and SWS and FWS time were longer than those in the control group. The differences were statistically significant (P<0.05). At 48 h and 72 h after intervention, the serum melatonin levels in the observation group were higher than those in the control group (P<0.05). At 72 h after intervention, the levels of SOD and MDA in the observation group were lower than those in the control group, and the GSH-px level was higher than that in the control group (P<0.05). Conclusion Ozone major autohemotherapy can promote melatonin release in rats with sleep deprivation, decrease serum SOD level and MDA level, and increase serum GSH-px level in rats.

Key words: Ozone, Autohemotherapy, Sleep deprivation, Rats, Melatonin, Oxidative stress