Progress in Animal Models of Ischemic Stroke

doi:10.12300/j.issn.1674-5817.2021.049

Abstract

Abstract:

Ischemic stroke refers to the ischemic necrosis or softening of limited brain tissue caused by cerebral blood circulation disorder, ischemia and hypoxia, resulting in corresponding neurological functional defects. Ischemic stroke is one of the primary causes of human disability, seriously threatens human health, and there is still no effective treatment by now. In order to study the pathogenesis of ischemic stroke and prevent and treat it better, it is very important to establish appropriate animal models.This paper aims to summarize the animal models of ischemic stroke and its advantages and disadvantages.

Key words: Ischemic stroke, Animal model, Research progress

CLC Number:

Q95-33

Bo DONG, Jiaxin LIU, Wei XIONG, Songqi TANG, Wei HUANG. Progress in Animal Models of Ischemic Stroke[J]. Laboratory Animal and Comparative Medicine, 2022, 42(1): 54-61.

Figures/Tables 2

References 51

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造模方法	模型动物	评价指标
线栓法	SD大鼠、Wistar大鼠、昆明小鼠、新西兰白兔、自发性高血压大鼠	神经功能评分；行为学测试（神经、肌肉运动功能、前庭运动功能）；脑组织切片病理形态学观察
开颅电凝阻断法	SD大鼠、BALB /c小鼠、新西兰白兔、巴马香猪、C57BL/6J小鼠	神经功能评分；行为学测试（神经、肌肉运动功能、前庭运动功能）；脑组织切片病理形态学观察
光化学致血栓形成法	昆明小鼠、Wistar大鼠、食蟹猴	观察行为学变化；血脑屏障的完整性及梗塞面积观察；HE染色
血栓形成法（自体血栓法）	家犬、Beagle犬、Wistar大鼠、家兔	神经功能评分；脑组织切片病理形态学观察；测定脑组织含水量及梗死体积率
FeCl₃致血栓形成法	新西兰白兔	行为改变；DSA图像表现；TTC染色测定脑梗死范围；脑组织切片病理形态学观察
栓子栓塞阻断法（硅胶柱/硅胶颗粒）	家犬、新西兰白兔	行为学观察；大脑微血管造影；脑组织切片病理形态学观察查
栓子栓塞阻断法（月硅酸钠）	新西兰白兔	CT灌注成像；脑组织切片病理形态学观察
栓子栓塞阻断法（中药提取）	Wistar大鼠	神经功能障碍检查评分；脑组织切片病理形态学观察
内皮素-1灌注致血管收缩法	SD大鼠	神经症状评分；24 h切出脑片，计算大脑半球体积及脑梗死体积；脑片作HE染色，计数存活神经元数
球囊导管法	猕猴	生命体征（体温、呼吸、心率）；Task-oriented神经行为功能评分；磁共振扫描和血管成像，计算脑梗塞体积
2根血管闭塞法	SD大鼠、长爪沙鼠	模型动物死亡率、行为学评分；脑组织细胞坏死率、凋亡率、炎症因子；脑组织切片病理形态学观察
3根血管闭塞法	Wistar大鼠	模型动物意识形态、生命体征；脑组织切片病理形态学观察
4根血管闭塞法	SD大鼠、日本大耳白兔、家猫、杂种犬、Wistar大鼠	模型动物生存情况、意识形态；脑组织切片病理形态学观察
心脏骤停法	SD大鼠	脑组织切片病理形态学观察，行免疫组化观察脑内炎症指标
颈动脉分流法	SD大鼠	脑电图检测；瞳孔颜色观察；平均动脉压；脑组织切片病理形态学观察

造模方法	模型动物	评价指标
线栓法	SD大鼠、Wistar大鼠、昆明小鼠、新西兰白兔、自发性高血压大鼠	神经功能评分；行为学测试（神经、肌肉运动功能、前庭运动功能）；脑组织切片病理形态学观察
开颅电凝阻断法	SD大鼠、BALB /c小鼠、新西兰白兔、巴马香猪、C57BL/6J小鼠	神经功能评分；行为学测试（神经、肌肉运动功能、前庭运动功能）；脑组织切片病理形态学观察
光化学致血栓形成法	昆明小鼠、Wistar大鼠、食蟹猴	观察行为学变化；血脑屏障的完整性及梗塞面积观察；HE染色
血栓形成法（自体血栓法）	家犬、Beagle犬、Wistar大鼠、家兔	神经功能评分；脑组织切片病理形态学观察；测定脑组织含水量及梗死体积率
FeCl₃致血栓形成法	新西兰白兔	行为改变；DSA图像表现；TTC染色测定脑梗死范围；脑组织切片病理形态学观察
栓子栓塞阻断法（硅胶柱/硅胶颗粒）	家犬、新西兰白兔	行为学观察；大脑微血管造影；脑组织切片病理形态学观察查
栓子栓塞阻断法（月硅酸钠）	新西兰白兔	CT灌注成像；脑组织切片病理形态学观察
栓子栓塞阻断法（中药提取）	Wistar大鼠	神经功能障碍检查评分；脑组织切片病理形态学观察
内皮素-1灌注致血管收缩法	SD大鼠	神经症状评分；24 h切出脑片，计算大脑半球体积及脑梗死体积；脑片作HE染色，计数存活神经元数
球囊导管法	猕猴	生命体征（体温、呼吸、心率）；Task-oriented神经行为功能评分；磁共振扫描和血管成像，计算脑梗塞体积
2根血管闭塞法	SD大鼠、长爪沙鼠	模型动物死亡率、行为学评分；脑组织细胞坏死率、凋亡率、炎症因子；脑组织切片病理形态学观察
3根血管闭塞法	Wistar大鼠	模型动物意识形态、生命体征；脑组织切片病理形态学观察
4根血管闭塞法	SD大鼠、日本大耳白兔、家猫、杂种犬、Wistar大鼠	模型动物生存情况、意识形态；脑组织切片病理形态学观察
心脏骤停法	SD大鼠	脑组织切片病理形态学观察，行免疫组化观察脑内炎症指标
颈动脉分流法	SD大鼠	脑电图检测；瞳孔颜色观察；平均动脉压；脑组织切片病理形态学观察

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