Abstract:

Objective To test the single nucleotide polymorphism (SNP) detection technology level of mice genetic quality control in laboratories, promote the application of nucleic acid samples and SNP detection technology by implementing a nationwide proficiency testing program. Methods In 2022, National Institutes for Food and Drug Control implemented domestic laboratory proficiency testing named“NIFDC-PT-365 Laboratory Proficiency Testing Program of SNP Marker Detection Ablity in Laboratory Mice DNA Samples”. According to the requirements of the program, each participating laboratory received 2 blind samples of DNA from BALB/c mice and C57BL/6 mice with random numbers, standard operation procedure and 2 pairs of primers for rs3023177 and rs3023382 SNP loci. The participants should submit the results and original records before the deadline. If the mutation site amplification and sequencing results were consistent with the preseted results, it was considered as satisfactory; otherwise, it was considered as unsatisfactory. Meanwhile, PCR amplification system, sequencing direction and alignment methods of participating laboratories were compared to analyze as the relevant factors which may affect the SNP detection results of nucleic acid standard samples. Results The results of 10 participating laboratories were consistent with the preset. They all obtained satisfactory results. Differences in amplification volume (20 μL, 25 μL, 30 μL), Taq enzyme system (single component and PCR MIX), sequencing company, sequencing direction and alignment software had no influence on the accuracy of the results. Conclusion The participating laboratories have high detection ability in SNP detection method of mice nucleic acid samples. SNP detection technology is simple and feasible in mouse genetic quality control and is worth popularizing.

Key words: Proficiency testing, Genetic quality control, Nucleic acid samples, Single nucleotide polymorphism, Mice