Effects of Pogostemon cablin on Serum Metabolomiceof Guizhou Miniature Pigs and It's mechanism

doi:10.12300/j.issn.1674-5817.2022.186

Abstract

Abstract:

Objective Based on the liquid chromatography-tandem mass spectormetry (LC-MS/MS), to study the effects of Pogostemon cablin on serum metabolism of Guizhou miniature pigs， and to explore its pharmacological mechanism. Methods Nine healthy Guizhou miniature pigs were divided into two groups, namely Pogostemon cablin drug group (n=5) and control group (n=4). The pigs in Pogostemon cablin drug group were orally fed with traditional Chinese medicine formula granules, each 0.5 g per day, for consecutive 8 days, while those in control group were given normal feeding without additional treatment. After the feeding experiment, serum samples were collected and analyzed using the LC-MS/MS technology. The metabolomics data was annotated and compared with KEGG, HMDB and Lipidmaps databases. Bioinformatics analysis methods including partial least squares discriminant analysis (PLS-DA), intergroup clustering, differential metabolite analysis and functional enrichment were used to screen differential metabolic biomarkers and their possible metabolic pathways. Results Forty-four differential metabolites (P<0.05) were screened out from the 443 metabolites, eight differential metabolites were significantly up-regulated (P<0.01), namely cinnamoylglycine, N-benzyl-N-isopropyl-N'-[4-(trifluoromethoxy) phenyl]urea, hypotaurine, D-glucose 6-phosphate, cis-2-decenoic acid, 11(Z),14(Z)-eicosadienoic acid, prostaglandin A2 and 10-hydroxydecanoic acid, and three differential metabolites were significantly down-regulated (P<0.01), namely lysophosphatidyl choline 22:5, lysophosphatidic acid 22:6 and lysophosphatidic acid 22:5. The differential metabolites were mainly enriched in the metabolic pathways of alanine, aspartate and glutamate metabolism (MapID: map00250) and taurine and hypotaurine metabolism (MapID: map00430). Conclusion Pogostemon cablin can significantly affect the metabolism of lysophosphatidic acids in porcine, and relieve the disorder of amino acids metabolism and regulate the occurrence of inflammation by affecting the metabolic pathways of alanine, aspartate and glutamate metabolism and taurine and hypotaurine metabolism.

Key words: Serum metabolomics, Pogostemon cablin, Guizhou miniature pig, Pharmacology of traditional Chinese medicine, Action mechanism

CLC Number:

R-332

Taofeng LU, Hui ZHANG, Jie ZHOU, Qian LI, Shuguang WU, Yanjun WU. Effects of Pogostemon cablin on Serum Metabolomiceof Guizhou Miniature Pigs and It's mechanism[J]. Laboratory Animal and Comparative Medicine, 2023, 43(3): 253-261.

Figures/Tables 5

Figure 1 Pairings correlation of QC samples in positive （A） and negative （B） ion modeNote：QC， Quality control sample； pos， Positive ion mode； neg， Negative ion mode.

Figure 2 The results of LC-MS/MS negative mode scanning total ion flow diagram （A） and PLS-DA score map （B） of serum metabolites of pigs after administration of Pogostemon cablinNote：A， LC-MS/MS negative mode scanning total ion flow diagram； B， Partial least squares discriminant analysis （PLS-DA） score map for metabolite data. GHX， Pogostemon cablin drug group given 0.5 g/head of traditional Chinese medicine formula granules per day by mixing materials through oral feeding for consecutive 8 days； CT， The control group given normal feeding without additional treatment； RT， Treatment time.

Figure 3 Annotation results of serum metabolites of pigs after administration of Pogostemon cablinNote：A to C are the comparative annotation results of HMDB （Human Metabolome Database） database， KEGG （Kyoto Encyclopedia of Genes and Genomes） database， and LIPID Maps database， respectively.

Figure 4 Volcanic map （A）， clustering heat map （B） and correlation analysis diagram （C） of differential serum metabolites of pigs after administration of Pogostemon cablinNote：GHX， Pogostemon cablin drug group given 0.5 g/head of traditional Chinese medicine formula granules per day by mixing materials through oral feeding for consecutive 8 days； CT， The control group given normal feeding without additional treatment； FC， Fold change.

Table 1 The basic information of the main differential metabolites of pigs after administration of Pogostemon cablin

代谢物 Metabolites	代谢物中文名称 Metabolites in Chinese	保留时间/min Retention time/min	log₂(FC)	P值 P value
Cinnamoylglycine	肉桂酰甘氨酸	8.378	3.670	0.001
N-benzyl-N-isopropyl-N'-[4-(trifluoromethoxy)phenyl] urea	N-苄基-N-异丙基-N'-(4-三氟甲基)苯尿素	4.541	2.217	0.005
Hypotaurine	亚牛磺酸	14.485	1.703	0.008
D-Glucose 6-phosphate	D-葡萄糖-6-磷酸	1.158	1.595	0.003
cis-2-Decenoic acid	顺-2-癸烯酸	12.057	1.536	0.002
11(Z),14(Z)-Eicosadienoic acid	二十碳二烯酸(顺-11,14)	14.820	1.349	0.007
Prostaglandin A2	前列腺素A2	12.727	1.289	0.008
10-Hydroxydecanoic acid	10-羟基癸烯酸	11.707	1.261	0.004
Lysophosphatidyl choline 22:5	溶血磷脂酰胆碱22:5	14.673	-2.190	0.002
Lysophosphatidyl acid 22:6	溶血磷脂酸22:6	14.010	-2.201	<0.001
Lysophosphatidyl acid 22:5	溶血磷脂酸22:5	14.139	-2.361	0.003

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