脾胃虚寒型胃溃疡动物模型研究进展

doi:10.12300/j.issn.1674-5817.2025.015

实验动物与比较医学 ›› 2025, Vol. 45 ›› Issue (5): 574-585.DOI: 10.12300/j.issn.1674-5817.2025.015

• 实验动物与中医药学专题 • 上一篇下一篇

脾胃虚寒型胃溃疡动物模型研究进展

刘子琪¹(), 李云英¹(), 李钦¹^,², 李元涵¹, 何芳雁¹^,²()(), 温伟波¹^,²()()

^1. 云南中医药大学中药学院, 昆明 650500
^2. 云南省中西医结合慢病防治重点实验室, 昆明 650500

收稿日期:2025-01-24 修回日期:2025-06-10 出版日期:2025-10-25 发布日期:2025-10-23
通讯作者:
何芳雁（1989—），女，硕士，高级实验师，研究方向：中药药理与应用。E-mail： hefangyan@foxmail.com。ORCID：0009-0009-8945-8002；

温伟波（1968—），男，硕士，教授，研究方向：中药民族药治疗代谢性疾病及新突发传染病研究。E-mail： wenweibo2020@163.com。ORCID：0009-0009-9759-3765
作者简介:刘子琪（2000—），女，硕士研究生，研究方向：中药药理与应用。E-mail：1947316311@qq.com。ORCID：0009-0003-1432-1657；
李云英（1997—），女，硕士研究生，研究方向：中药药理与应用。E-mail：2594903717@qq.com。ORCID：0009-0009-3030-237X
基金资助:
国家重点研发计划“中医证候动物模型的建立及其在中药/民族药评价中的应用研究”(2023YFF0724803)

Research Progress on Animal Models of Gastric Ulcer of Spleen-Stomach Deficiency Cold Type

LIU Ziqi¹(), LI Yunying¹(), LI Qin¹^,², LI Yuanhan¹, HE Fangyan¹^,²()(), WEN Weibo¹^,²()()

^1. College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China
^2. Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease Prevention and Control, Kunming 650500, China

Received:2025-01-24 Revised:2025-06-10 Published:2025-10-25 Online:2025-10-23
Contact: HE Fangyan (ORCID: 0009-0009-8945-8002), E-mail: hefangyan@foxmail.com;
WEN Weibo (ORCID: 0009-0009-9759-3765), E-mail: wenweibo2020@163.com;

摘要/Abstract

摘要：

胃溃疡（gastric ulcer，GU）是消化系统的常见病、多发病和顽固性疾病之一。脾胃虚寒是GU最常见且难以治愈的一种证型，是医学研究领域的重点和难点。因此，构建科学合理且符合临床实际的脾胃虚寒型GU动物模型并制定客观有效的评价标准，对于深入研究GU的发病机制和治疗方法具有重要意义。本文通过系统梳理相关文献，对脾胃虚寒型GU动物模型的制备方法进行了全面介绍：首先介绍西医GU病理模型的构建方法，如幽门结扎法、水浸-束缚应激法、乙醇诱导法、乙酸诱导法等；阐述中医脾胃虚寒证候模型的建立方法，包括饮食失节法、苦寒泻下法、过度劳倦法、耗气破气法和过食酸味法等；重点讲述脾胃虚寒型GU病证结合模型的构建方法，包括双因素造模法和三因素造模法。同时，从多个角度总结了脾胃虚寒型GU动物模型的评价指标，包括动物体征表征（外观症状、动物行为和代谢指标）、组织形态与分子生物学相关指标（胃功能、氧化应激、炎性因子、其他细胞因子、凝血4项、肠道菌群检测），力求构建一个综合性的评价体系。本文进一步从方药反证的角度，通过分析方剂的药物组成和药理作用效果，推断其治疗的疾病动物模型所属的证型，从而验证动物模型是否构建成功。通过本文综述，以期为构建与临床实际高度契合、体现中医药特色的GU中医证候动物模型提供有力参考，从而推动GU中医证候研究和中医药治疗GU方法的深入探索，促进中医药在GU治疗中的应用和发展。

关键词: 胃溃疡, 脾胃虚寒, 病证结合, 动物模型

Abstract:

Gastric ulcer (GU) is one of the common, frequently-occurring and intractable diseases of the digestive system. Spleen-stomach deficiency cold type is the most common and hard-to-cure syndrome pattern of GU, and is both a focus and a challenge in medical research. Therefore, constructing a scientific, reasonable, and clinically practical animal model of GU with spleen-stomach deficiency cold type and formulating objective and effective evaluation criteria are of great significance for in-depth research on the pathogenesis and treatment of GU. In this paper, the methods for constructing GU animal models of spleen-stomach deficiency cold type are comprehensively introduced by systematically reviewing the relevant literature. Firstly, the construction methods of pathological models of GU in Western medicine are introduced, including pyloric ligation method, water immersion-restraint stress method, ethanol-induced method, acetic acid-induced method, etc. This paper expounds the establishment methods for spleen-stomach deficiency cold syndrome type model in traditional Chinese medicine (TCM), including diet disorder method, bitter cold diarrhea method, excessive fatigue method, Qi consumption and Qi impairment method, and overeating sour-flavor method. This paper focuses on the construction methods for disease-syndrome combination GU models of spleen-stomach deficiency cold type, including two-factor modeling method and three-factor modeling method. Meanwhile, the evaluation indices of GU animal models of spleen-stomach deficiency cold type were summarized from various aspects, including animal physical signs ( appearance symptoms, animal behavior, and metabolic indices), as well as tissue morphology and molecular biology-related indicators ( gastric function, oxidative stress, inflammatory factors, other cytokines, four coagulation parameters, intestinal flora detection ), for constructing a comprehensive evaluation system. From the perspective of prescription-based verification, this paper further analyzes the drug composition and pharmacological effects to infer the syndrome type of the treated animal model, so as to verify whether the target animal model is successfully constructed. This review aims to provide a valuable reference for establishing a syndrome-specific GU animal model that closely aligns with clinical reality and embodies the principles of Chinese medicine. This will further advance research on TCM-pattern GU syndromes and deepen the exploration of herbal medicine-based treatments for GU, ultimately promoting the clinical integration and advancement of Chinese medicine in GU therapy.

Key words: Gastric ulcer, Spleen-stomach deficiency cold, Disease-syndrome combination, Animal model

中图分类号:

R-332

刘子琪,李云英,李钦,等. 脾胃虚寒型胃溃疡动物模型研究进展[J]. 实验动物与比较医学, 2025, 45(5): 574-585. DOI: 10.12300/j.issn.1674-5817.2025.015.

LIU Ziqi,LI Yunying,LI Qin,et al. Research Progress on Animal Models of Gastric Ulcer of Spleen-Stomach Deficiency Cold Type[J]. Laboratory Animal and Comparative Medicine, 2025, 45(5): 574-585. DOI: 10.12300/j.issn.1674-5817.2025.015.

图/表 5

参考文献 56

[1]	GONG H Y , ZHAO N , ZHU C L , et al . Treatment of gastric ulcer, traditional Chinese medicine may be a better choice[J]. J Ethnopharmacol. 2024, 324:117793. DOI:10.1016/j.jep.2024.117793 .
[2]	LI Z S , ZOU D W , MA X Q , et al . Epidemiology of peptic ulcer disease: endoscopic results of the systematic investigation of gastrointestinal disease in China[J]. Am J Gastroenterol, 2010, 105(12):2570-2577. DOI:10.1038/ajg.2010.324 .
[3]	薛彩霞, 张雁霞 . 714例上消化道出血的临床特点、病因及危险因素分析[J]. 山西大同大学学报(自然科学版), 2022, 38(3):73-75, 85. DOI:10.3969/j.issn.1674-0874.2022.03.016 .
	XUE C X , ZHANG Y X . Clinical characteristics, pathogeny and risk factors of 714 cases of upper gastrointestinal bleeding[J]. J Shanxi Datong Univ Nat Sci Ed, 2022, 38(3):73-75, 85. DOI:10.3969/j.issn.1674-0874.2022.03.016 .
[4]	JIN Y , ZHANG M , WANG Y F , et al . Protective effect and potential mechanism of the traditional Chinese medicine Shaoyao-Gancao decoction on ethanol-induced gastric ulcers in rats[J]. Evid Based Complement Alternat Med, 2022, 2022:3069089. DOI:10.1155/2022/3069089 .
[5]	WU T T , ZHANG H , JIN Y , et al . The active components and potential mechanisms of Wuji Wan in the treatment of ethanol-induced gastric ulcer: an integrated metabolomics, network pharmacology and experimental validation[J]. J Ethnopharmacol, 2024, 23;326:117901. DOI:10.1016/j.jep.2024.117901 .
[6]	高月, 李佳 . 黄芪建中汤对老年脾胃虚寒型胃溃疡患者的临床疗效及血清EGF、TIMP-1及PGE2的影响[J]. 中医药学报, 2025, 53(1):93-97. DOI: 10.19664/j.cnki.1002-2392.250017 .
	GAO Y , LI J . Clinical efficacy of Huangqi Jianzhong decoction on elderly patients with spleen and stomach deficiency-cold type gastric ulcer and its effects on serum EGF, TIMP-1, and PGE2 levels[J]. Acta Chin Med Pharmacol, 2025, 53(1):93-97. DOI: 10.19664/j.cnki.1002-2392.250017 .
[7]	胡羽, 肖景东 . 中医中药治疗胃溃疡的研究进展[J]. 中医临床研究, 2017, 9(29):142-145. DOI: 10.3969/j.issn.1674-7860.2017.29.071 .
	HU Y , XIAO J D . A review on treating gastric ulcer in TCM[J]. Clin J Chin Med, 2017, 9(29):142-145. DOI: 10.3969/j.issn.1674-7860.2017.29.071 .
[8]	扈丽萍 . 李慧臻主任中医治疗胃溃疡经验初探[J]. 光明中医, 2021, 36(14):2313-2315. DOI: 10.3969/j.issn.1003-8914.2021.14.015 .
	HU L P . Discussion on the experience of director Li Huizhen in treating gastric ulcer with traditional Chinese medicine[J]. Guangming J Chin Med, 2021, 36(14):2313-2315. DOI: 10.3969/j.issn.1003-8914.2021.14.015 .
[9]	黄志强, 温孝荣 . 黄芪建中汤联合督灸对脾胃虚寒型胃溃疡的影响[J]. 光明中医, 2024, 39(23):4772-4774. DOI: 10.3969/j.issn.1003-8914.2024.23.032 .
	HUANG Z Q , WEN X R . Effect of Huangqi Jianzhong decoction combined with governor vessel moxibustion on gastric ulcer of spleen and stomach deficiency-cold type[J]. Guangming J Chin Med, 2024, 39(23):4772-4774. DOI: 10.3969/j.issn.1003-8914.2024.23.032 .
[10]	宋厚盼, 曾梅艳, 陈小娟, 等 . 脾胃虚寒型胃溃疡病证结合大鼠实验模型的建立及评价研究[J]. 中国中医基础医学杂志, 2020, 26(4):468-473. DOI: CNKI:SUN:ZYJC.0.2020-04-019 .
	SONG H P , ZENG M Y , CHEN X J , et al . Establishment and evaluation of the combination of disease and syndrome experimental model of spleen stomach deficiency cold type of gastric ulcer in rats[J]. Chin J Basic Med Tradit Chin Med, 2020, 26(4):468-473. DOI: CNKI:SUN:ZYJC.0.2020-04-019 .
[11]	胥文娟, 汪龙德, 牛媛媛, 等 . 消化系统病证结合动物模型的研究进展及中医药靶点干预[J]. 中国实验方剂学杂志, 2022, 28(19):258-266. DOI: 10.13422/j.cnki.syfjx.20221845 .
	XU W J , WANG L D , NIU Y Y , et al . Research progress of digestive system disease and syndrome combined animal model and traditional Chinese medicine target intervention: a review[J]. Chin J Exp Tradit Med Formulae, 2022, 28(19):258-266. DOI: 10.13422/j.cnki.syfjx.20221845 .
[12]	刘霞, 李静, 戴纯辉, 等 . 牛乳铁蛋白对实验性胃黏膜损伤及胃溃疡大鼠模型的影响[J]. 中国现代应用药学, 2022, 39(6):757-763. DOI: 10.13748/j.cnki.issn1007-7693.2022.06.007 .
	LIU X , LI J , DAI C H , et al . Influence of bovine lactoferrin effects on experimental gastric mucosal injury and gastric ulcer rat model[J]. Chin J Mod Appl Pharm, 2022, 39(6):757-763. DOI: 10.13748/j.cnki.issn1007-7693.2022.06.007 .
[13]	ABDEL-HAMED A R , ABO-ELMATTY D M , ESSAWY S S , et al . Antisecretory and antioxidative effects of the antidepressants fluvoxamine and mirtazapine on water immersion stress and pyloric ligation-induced gastric ulcer in rats[J]. Int J Health Sci, 2022, 16(3):25-34.
[14]	AL-GABRI N , ELNAGAR G M , SAGHIR S A M , et al . Preliminary study of gastroprotective effect of Aloe perryi and date palm extracts on pyloric ligation-induced gastric ulcer in experimental rats[J]. Biomed Res Int, 2022, 2022(1):9246785. DOI:10.1155/2022/9246785 .
[15]	ZHANG D J , XIANG M , JIANG Y , et al . The protective effect of polysaccharide SAFP from Sarcodon aspratus on water immersion and restraint stress-induced gastric ulcer and modulatory effects on gut microbiota dysbiosis[J]. Foods, 2022, 11(11):1567. DOI:10.3390/foods11111567 .
[16]	FIGUEIREDO F F , DAMAZO A S , ARUNACHALAM K , et al . Evaluation of the gastroprotective and ulcer healing properties by Fridericia chica (Bonpl.) L.G. Lohmann hydroethanolic extract of leaves. J Ethnopharmacol. 2023 Jun 12;309:116338. DOI: 10.1016/j.jep.2023.116338 .
[17]	AKMAL M N , ABDEL AZIZ I , AZLINA M F NUR . Piper sarmentosum Roxb. methanolic extract prevents stress-induced gastric ulcer by modulating oxidative stress and inflammation[J]. Front Pharmacol, 2023, 13:971443. DOI:10.3389/fphar.2022.971443 .
[18]	EDITORS P O . Retraction: antioxidant properties and gastroprotective effects of 2-(ethylthio)benzohydrazones on ethanol-induced acute gastric mucosal lesions in rats[J]. PLoS One, 2023, 18(11): e0294011. DOI:10.1371/journal.pone. 0294011 .
[19]	GHAREEB M A , MOHAMMED H S , ABOUSHOUSHA T , et al . Ipomoea carnea mitigates ethanol-induced ulcers in irradiated rats via Nrf2/HO-1 pathway: an in vivo and in silico study[J]. Sci Rep. 2024, 14(1):3469. DOI: 10.1038/s41598-024-53336-1 .
[20]	HAN Y M , SONG M Y , LEE D Y , et al . Gastric mucosal protective effects of Cinnamomum cassia in a rat model of ethanol-induced gastric injury[J]. Nutrients, 2023, 16(1):55. DOI:10.3390/nu16010055 .
[21]	姚晓艳, 刘文, 宋信莉, 等 . 左金对乙醇诱导致大鼠胃溃疡的治疗作用及机制研究[J]. 中华中医药学刊, 2019, 37(10):2404-2407, 2565. DOI: 10.13193/j.issn.1673-7717.2019.10.023 .
	YAO X Y , LIU W , SONG X L , et al . Effect and mechanism of Zuojin pills on gastric ulcer induced by ethanol in rats[J]. Chin Arch Tradit Chin Med, 2019, 37(10):2404-2407, 2565. DOI: 10.13193/j.issn.1673-7717.2019.10.023 .
[22]	LIU H F , CHEN Y Q , HU Y L , et al . Protective effects of an alcoholic extract of Kaempferia Galanga L. rhizome on ethanol-induced gastric ulcer in mice[J]. J Ethnopharmacol, 2024, 325:117845. DOI:10.1016/j.jep.2024.117845 .
[23]	樊继法, 曾海松, 刘吉华 . 荜铃胃痛颗粒抗实验性胃溃疡作用研究[J]. 药物评价研究, 2022, 45(2):259-265. DOI: 10.7501/j.issn.1674-6376.2022.02.008 .
	FAN J F , ZENG H S , LIU J H . Effect of Biling Weitong Granules in treating experimental gastric ulcer[J]. Drug Eval Res, 2022, 45(2):259-265. DOI: 10.7501/j.issn.1674-6376.2022.02.008 .
[24]	IPEK E , EKREN AŞICI G S , EPIKMEN E T , et al . Evaluation of acetic acid-induced chronic gastric ulcer healing by propionyl-L-carnitine administration[J]. Chem Biodivers, 2023, 20(7): e202300210. DOI:10.1002/cbdv.202300210 .
[25]	王建兵, 刘二军, 张伟玲, 等 . 电针联合健中愈疡片对实验性胃溃疡模型大鼠β-内啡肽及炎症因子的影响[J]. 东南大学学报(医学版), 2018, 37(5):925-929. DOI: 10.3969/j.issn.1671-6264.2018.05.037 .
	WANG J B , LIU E J , ZHANG W L , et al . Effects of electroacupuncture plus Jianzhongyuyang Pill on β-endorphin and inflammatory factor in experimental gastric ulcer model rats[J]. J Southeast Univ Med Sci Ed, 2018, 37(5):925-929. DOI: 10.3969/j.issn.1671-6264.2018.05.037 .
[26]	马平平, 毕珺辉, 徐丹, 等 . 良附丸对4种实验性胃溃疡模型大鼠的防治效果[J]. 中医药信息, 2018, 35(5):9-12. DOI: 10.19656/j.cnki.1002-2406.180053 .
	MA P P , BI J H , XU D , et al . Therapeutic and preventive effects of Liangfu Wan on four experimental gastric ulcer models in rats[J]. Inf Tradit Chin Med, 2018, 35(5):9-12. DOI: 10.19656/j.cnki.1002-2406.180053 .
[27]	范东东, 郑丰杰 . 从«伤寒论·平脉法»第32条谈中焦寒邪的上逆与下溜[J]. 北京中医药大学学报, 2018,41(1): 5-8.DOI:10.3969/j.issn.1006-2157.2018.01.001 .
	FAN D D , ZHENG F J . On ascending counterflow and descending slide of middle energizer cold base on item 32 in Shanghan Lun·Pingmai Fa[J]. J Beijing Univ Tradit Chin Med, 2018,41 (1): 5-8. DOI:10.3969/j.issn.1006-2157.2018.01.001 .
[28]	仇婧玥, 喻昶, 熊萌, 等 . 理中汤干预MAPK通路治疗脾胃虚寒型胃溃疡大鼠的机制研究[J]. 中药药理与临床, 2025, 41(4):2-9. DOI: 10.13412/j.cnki.zyyl.20241012.002 .
	QIU J Y , YU C , XIONG M , et al . Pharmacodynamic mechanism of Lizhong decoction in treatment of gastric ulcer due to piweixuhan in rats via MAPK signaling pathway[J]. Pharmacol Clin Chin Mater Med, 2025, 41(4):2-9. DOI: 10.13412/j.cnki.zyyl.20241012.002 .
[29]	白敏, 段永强, 杨晓轶, 等 . 黄芪建中汤对脾胃虚寒型胃溃疡大鼠作用机制研究[J]. 中国临床药理学杂志, 2021, 37(4):397-400. DOI: 10.13699/j.cnki.1001-6821.2021.04.012 .
	BAI M , DUAN Y Q , YANG X Y , et al . Effect and mechanism of Huangqi Jianzhong decoction on gastric ulcer of spleen stomach deficiency cold type in rats[J]. Chin J Clin Pharmacol, 2021, 37(4):397-400. DOI: 10.13699/j.cnki.1001-6821.2021.04.012 .
[30]	张瑞欣, 杨志军, 黄蓉, 等 . 不同配比生姜-黄芩药对对胃寒证模型大鼠能量代谢酶、胃肠动力及脑肠肽的影响[J]. 中医杂志, 2022, 63(22):2162-2169. DOI: 10.13288/j.11-2166/r.2022.22.013 .
	ZHANG R X , YANG Z J , HUANG R , et al . Effects of Shengjiang (Rhizoma Zingiberis Recens)-Huangqin (Radix Scutellariae) herbal pair at different composition ratios on energy metabolism enzymes, gastrointestinal motility and brain-gut peptides in rats with stomach cold syndrome[J]. J Tradit Chin Med, 2022, 63(22):2162-2169. DOI: 10.13288/j.11-2166/r.2022.22.013 .
[31]	吴春艳, 邢陈 . 康复新液联合黄芪建中汤治疗胃溃疡对炎性因子、氧化应激的影响[J]. 中医药临床杂志, 2022, 34(12):2363-2366. DOI: 10.16448/j.cjtcm.2022.1237 .
	WU C Y , XING C . Observation on therapeutic effect of kangfuxin liquid combined with Huangqi Jianzhong decoction on gastric ulcer and its influence on inflammatory factors and oxidative stress[J]. Clin J Tradit Chin Med, 2022, 34(12):2363-2366. DOI: 10.16448/j.cjtcm.2022.1237 .
[32]	李希新 . 对苦寒药伤胃的理论探讨[J]. 中国中药杂志, 2001, 26(11): 792-794. DOI: 10.3321/j.issn:1001-5302.2001.11.028 .
	LI X X . A theoretical exploration on stomach impairment by bitter-cold herbs[J]. China J Chin Mater Med, 2001, 26(11): 792-794. DOI: 10.3321/j.issn:1001-5302.2001.11.028 .
[33]	谢建群, 陆雄, 马军, 等 . 健脾温中法对脾胃虚寒型肠易激综合征模型大鼠生长抑素影响的实验研究[J]. 江西中医学院学报, 2003, 15(4):56-58. DOI: 10.3969/j.issn.1005-9431.2003.04.022 .
	XIE J Q , LU X , MA J , et al . Experimental Study on the Influence upon Somatostatin in Rats of Irritable Bo wel Syndrome of insufficiency of the spleen-Yang by Invigorating Spleen and Warming Mid-energizer[J]. J Jiangxi Coll Tradit Chin Med, 2003, 15(4):56-58. DOI: 10.3969/j.issn.1005-9431.2003.04.022 .
[34]	陈小娟, 曾梅艳, 宋厚盼, 等 . 黄芪建中汤对脾胃虚寒证胃溃疡大鼠的影响[J]. 中成药, 2020, 42(4):867-874. DOI: 10.3969/j.issn.1001-1528.2020.04.009 .
	CHEN X J , ZENG M Y , SONG H P , et al . Effects of Huangqi jianzhong decoction on rats with gastric ulcer of spleen stomach deficiency and cold pattern[J]. Chin Tradit Pat Med, 2020, 42(4):867-874. DOI: 10.3969/j.issn.1001-1528.2020.04.009 .
[35]	孙虎, 丁永伟, 王平, 等 . 脾胃气虚型胃溃疡大鼠病证结合模型的制备[J]. 山东中医杂志, 2015, 34(1):47-50. DOI: 10.16295/j.cnki.0257-358x.2015.01.032 .
	SUN H , DING Y W , WANG P , et al . Preparation of the gastric ulcer rat model with deficiency of spleen and stomach[J]. Shandong J Tradit Chin Med, 2015, 34(1):47-50. DOI: 10.16295/j.cnki.0257-358x.2015.01.032 .
[36]	韩运宗, 陈思清, 刘琴, 等 . 黄芪建中汤对脾胃虚寒证胃溃疡大鼠炎症因子及HGF/c-Met信号通路的影响[J]. 中国中医急症, 2024, 33(1):22-26. DOI: 10.3969/j.issn.1004-745X.2024.01.005 .
	HAN Y Z , CHEN S Q , LIU Q , et al . Effects of Huangqi Jianzhong decoction on inflammatory factors and HGF/c-met signaling pathway in rats with gastric ulcer of spleen-stomach deficiency and cold syndrome[J]. J Emerg Tradit Chin Med, 2024, 33(1):22-26. DOI: 10.3969/j.issn.1004-745X.2024.01.005 .
[37]	陈思清, 周赛男, 韩运宗, 等 . 黄芪建中汤对胃溃疡大鼠血清炎症因子、胃黏膜表皮生长因子受体表达的影响[J]. 陕西中医, 2023, 44(8):1032-1036. DOI: 10.3969/j.issn.1000-7369.2023.08.008 .
	CHEN S Q , ZHOU S N , HAN Y Z , et al . Effect of Huangqi Jianzhong decoction on serum inflammatory factors and expression of gastric mucosal EGFR in rats with gastric ulcer[J]. Shaanxi J Tradit Chin Med, 2023, 44(8):1032-1036. DOI: 10.3969/j.issn.1000-7369.2023.08.008 .
[38]	周赛男, 刘琴, 周姝, 等 . 黄芪建中汤对脾胃虚寒证胃溃疡大鼠胃黏膜保护作用的机制研究[J]. 湖南中医药大学学报, 2023, 43(11):1978-1985. DOI: 10.3969/j.issn.1674-070X.2023.11.007 .
	ZHOU S N , LIU Q , ZHOU S , et al . Mechanism of action of Huangqi jianzhong decoction in protecting gastric mucosa of gastric ulcer rats with deficiency cold of the spleen and stomach pattern[J]. J Hunan Univ Chin Med, 2023, 43(11):1978-1985. DOI: 10.3969/j.issn.1674-070X.2023.11.007 .
[39]	何杰滢, 桂蓓, 李梦秋, 等 . 胃寒型功能性消化不良大鼠模型的构建[J]. 中药药理与临床, 2022, 38(2):212-217. DOI: 10.13412/j.cnki.zyyl.2022.02.028 .
	HE J Y , GUI P , LI M Q , et al . Construction of functional dyspepsia models of Weihan syndrome in rats[J]. Pharmacol Clin Chin Mater Med, 2022,38(2):212-217. DOI: 10.13412/j.cnki.zyyl.2022.02.028 .
[40]	邵占梅, 仲继冬, 房玉明, 等 . 格瓦斯对脾胃虚寒型胃溃疡大鼠保护作用的研究[J]. 中国酿造, 2022, 41(1):64-69. DOI: 10.11882/j.issn.0254-5071.2022.01.011 .
	SHAO Z M , ZHONG J D , FANG Y M , et al . Protective effect of Kvass on gastric ulcer rats with spleen-stomach deficiency[J]. China Brew, 2022, 41(1):64-69. DOI: 10.11882/j.issn.0254-5071.2022.01.011 .
[41]	余凌英, 李星, 蔡平君, 等 . 干姜和炮姜对脾胃虚寒型胃溃疡大鼠药效学指标及肠道菌群的影响[J]. 中国药房, 2022, 33(20):2460-2465. DOI: 10.6039/j.issn.1001-0408.2022.20.06 .
	YU L Y , LI X , CAI P J , et al . Effects of Zingiber officinale and processed Zingiber officinale on pharmacodynamic indexes and intestinal flora on gastric ulcer rats with spleen-stomach deficiency and cold type[J]. China Pharm, 2022, 33(20):2460-2465. DOI: 10.6039/j.issn.1001-0408.2022.20.06 .
[42]	秦华珍, 李明芳, 丘海冰, 等 . 红豆蔻、大高良姜乙酸乙酯部位对胃溃疡寒证大鼠胃组织GAS、MOT、EGF、VEGF、6-keto-PGF1α的影响[J]. 中华中医药杂志, 2018, 33(9):3886-3889. DOI: CNKI:SUN:BXYY.0.2018-09-043 .
	QIN H Z , LI M F , QIU H B , et al . Effects of ethyl acetate parts of Alpinia galanga Will. and Alpinia galangal(L.) Will on GAS, MOT, EGF, VEGF and 6-keto-PGF1α contents in gastro-tissue of gastric ulcer rats with cold syndrome[J]. China J Tradit Chin Med Pharm, 2018, 33(9):3886-3889. DOI: CNKI:SUN:BXYY.0.2018-09-043 .
[43]	柳俊辉, 罗君, 谢鹏, 等 . 5味山姜属中药不同部位对胃溃疡寒证大鼠环核苷酸系统的影响[J]. 时珍国医国药, 2017, 28(12):2831-2833. DOI: 10.3969/j.issn.1008-0805.2017.12.006 .
	LIU J H , LUO J , XIE P , et al . Effects of 5 kinds of Chinese herbal medicine Alpinia in different parts of gastric ulcer of rats with cold cyclic nucleotide system[J]. Lishizhen Med Mater Med Res, 2017, 28(12):2831-2833. DOI: 10.3969/j.issn.1008-0805.2017.12.006 .
[44]	秦华珍, 牛新迈, 谢旭格, 等 . 高良姜、大高良姜黄酮类成分对胃溃疡寒证大鼠胃组织PGE2、EGF和血清IL-8、TNF-α等指标的影响[J]. 时珍国医国药, 2022, 33(11):2612-2614. DOI: 10.3969/j.issn.1008-0805.2022.11.13 .
	QIN H Z , NIU X M , XIE X G , et al . Effects of flavonoids from Alpinia officinarum and Alpinia officinarum on PGE2, EGF, and serum IL-8, TNF-α levels in rats with gastric ulcer cold syndrome[J]. Lishizhen Med Mater Med Res, 2022, 33(11):2612-2614. DOI: 10.3969/j.issn.1008-0805.2022.11.13 .
[45]	魏广义, 刘楠楠, 王淑美, 等 . 抗胃溃疡中药实验研究进展[J]. 中药药理与临床, 2020, 36(5):228-232. DOI: 10.13412/j.cnki.zyyl.2020.05.029 .
	WEI G Y , LIU N N , WANG S M , et al . Experimental progress on traditional Chinese medicines against gastric ulcer[J]. Pharmacol Clin Chin Mater Med, 2020, 36(5):228-232. DOI: 10.13412/j.cnki.zyyl.2020.05.029 .
[46]	马玉朋, 袁亚茹, 邸志权, 等 . 功能性消化不良动物模型的中医证型及其评价指标研究进展[J]. 药物评价研究, 2024, 47(4):889-896. DOI:10.7501/j.issn.1674-6376.2024.04.022 .
	MA Y P , YUAN Y R , DI Z Q , et al . Research progress on animal models of functional dyspepsia in traditional Chinese medicine syndromes and evaluation indicators[J]. Drug Eval Res, 2024, 47(4):889-896. DOI:10.7501/j.issn.1674-6376.2024.04.022 .
[47]	吉琳, 申琪, 郭向东 . 阳虚动物模型及检测指标的研究进展[J]. 中国比较医学杂志, 2021, 31(1):138-145. DOI: 10.3969/j.issn.1671-7856.2021.01.021 .
	JI L , SHEN Q , GUO X D . Research progress in developing an animal model and detection index for Yang deficiency[J]. Chin J Comp Med, 2021, 31(1):138-145. DOI: 10.3969/j.issn.1671-7856.2021.01.021 .
[48]	尉光艳 . 基于颜色模型下不同证型胃脘痛患者舌象客观化特征研究[D]. 沈阳: 辽宁中医药大学, 2022. DOI: 10.27213/d.cnki.glnzc.2022.000214 .
	WEI G Y . Study on the objective characteristics of tongue image in patients with different syndromes of epigastric pain based on color model[D]. Shenyang : Liaoning University of Traditional Chinese Medicine, 2022. DOI: 10.27213/d.cnki.glnzc.2022.000214 .
[49]	魏晴, 梁珊珊, 熊瑞, 等 . 艳山姜提取物对急性胃溃疡模型小鼠的保护作用研究[J]. 中国药房, 2020, 31(18):2190-2195. DOI: 10.6039/j.issn.1001-0408.2020.18.03 .
	WEI Q , LIANG S S , XIONG R , et al . Study on the protective effect of Alpinia zerumbet extract on acute gastric ulcer model mice[J]. China Pharm, 2020, 31(18):2190-2195. DOI: 10.6039/j.issn.1001-0408.2020.18.03 .
[50]	王璐瑶, 邓文艳, 周丹, 等 . 电针合募配穴对胃溃疡模型大鼠血清生长抑素、胃泌素表达的影响[J]. 时珍国医国药, 2021, 32(10):2543-2547. DOI: 10.3969/j.issn.1008-0805.2021.10.67 .
	WANG L Y , DENG W Y , ZHOU D , et al . Effect of electroacupuncture at He-Mu acupoint combination on serum somatostatin and gastrin expression in gastric ulcer model rats[J]. Lishizhen Med Mater Med Res, 2021, 32(10):2543-2547. DOI: 10.3969/j.issn.1008-0805.2021.10.67 .
[51]	黄焕迪, 何瑞坤, 戴庆玲, 等 . 高通量测序研究3味山姜属中药黄酮类成分对胃溃疡寒证大鼠肠道菌群的影响[J]. 中华中医药学刊, 2022, 40(01):103-106+270-274.DOI:10.13193/j.issn.1673-7717.2022.01.025 .
	HUANG H D , HE R K , DAI Q L , et al . High-throughput sequencing study on effect of flavonoids from three kinds of Alpinia Herbs on Intestinal flora of rats with gastric ulcer cold syndrome s[J]. Chin Arch Tradit Chin Med, 2022,40(01):103-106+270-274.DOI:10.13193/j.issn.1673-7717.2022.01.025 .
[52]	秦百君, 唐曦平, 杨昕, 等 . 清解化攻方调节重症急性胰腺炎模型大鼠肠道菌群及对肠黏膜屏障的影响[J]. 中国药房, 2022, 33(15):1825-1832. DOI:10.6039/j.issn.1001-0408.2022.15.07 .
	QIN B J , TANG X P , YANG X , et al . Study on the effects of Qingjie huagong decoction on the regulation of intestinal flora and intestinal mucosal barrier in severe acute pancreatitis model rats[J]. China Pharm, 2022, 33(15):1825-1832. DOI:10.6039/j.issn.1001-0408.2022.15.07 .
[53]	GANGAIAH D , RYAN V , VAN HOESEL D , et al . Recombinant Limosilactobacillus (Lactobacillus) delivering nanobodies against Clostridium perfringens NetB and alpha toxin confers potential protection from necrotic enteritis[J]. Microbiology-Open, 2022, 11(2):e1270. DOI: 10.1002/mbo3.1270 .
[54]	仇婧玥 . 基于高通量mRNA测序探讨理中汤对胃溃疡脾胃虚寒证大鼠的干预机制[D]. 长沙: 湖南中医药大学, 2023. DOI: 10.27138/d.cnki.ghuzc.2023.000227 .
	QIU J Y . Based on high-throughput mRNA sequencing, the intervention mechanism of Lizhong decoction on gastric ulcer rats with spleen-stomach deficiency-cold syndrome was discussed[D]. Changsha: Hunan University of Chinese Medicine, 2023. DOI: 10.27138/d.cnki.ghuzc.2023.000227 .
[55]	瞿琪, 姜瑞, 陈祎洲, 等 . 基于"寒者热之"探讨温针灸在膝骨关节炎治疗中的应用[J]. 亚太传统医药, 2024, 20(10):234-237. DOI:10.11954/ytctyy.202410052 .
	QU Q , JIANG R , CHEN Y Z , et al . Application of warm acupuncture in the treatment of knee osteoarthritis: exploring the concept of warming the cold[J]. Asia Pac Tradit Med, 2024, 20(10):234-237. DOI: 10.11954/ytctyy.202410052 .
[56]	白敏, 段永强, 杨晓轶, 等 . 黄芪建中汤对脾胃虚寒型胃溃疡模型大鼠JAK2/STAT3信号通路的影响[J]. 中国实验方剂学杂志, 2020, 26(20):32-38. DOI: 10.13422/j.cnki.syfjx.20201908 .
	BAI M , DUAN Y Q , YANG X Y , et al . Effect of Huangqi Jianzhongtang on JAK2/STAT3 signal pathway in rats with spleen-stomach deficiency cold type gastric ulcer[J]. Chin J Exp Tradit Med Formulae, 2020, 26(20):32-38. DOI: 10.13422/j.cnki.syfjx.20201908 .

方法 Methods	优点 Advantages	缺点 Disadvantages	适用范围 Range of application	病理 Pathology
幽门结扎法 Pyloric ligation method	GU重复性好，实验结果稳定；实验造成的GU部位明确，溃疡程度可控	人为结扎为非生理性GU；实验操作技术要求高	急性GU研究；用于抗GU药物评估；GU相关机制研究	通过胃酸堆积、压迫血管，模拟GU
水浸-束缚应激法 Water immersion-restraint stress method	GU复制率高，重复性好；实验操作简单；能较好模拟应激引发的GU	操作过程缺乏量化标准；可能引发其他生理变化；个体差异影响较大；仅适用于短期研究	应激性GU研究；用于抗应激性GU药物评估；应激反应机制研究	通过束缚、水浸，模拟情志失调模型
乙醇诱导法 Ethanol-induced method	操作方法简单，重复性好，成模效果好；能短时间诱导胃黏膜损伤；GU溃疡程度可控	直接损伤胃黏膜为非生理性溃疡；不适用于长期慢性GU研究；死亡率较高	急性GU研究；用于抗GU、胃黏膜保护药物评估；胃黏膜屏障功能机制研究	通过乙醇灌胃，模拟寒邪或热邪侵袭
乙酸诱导法 Acetic acid-induced method	GU形成后持续时间长，重复性好；造模方法简单；溃疡程度可控	直接损伤胃黏膜为非生理性溃疡；诱导GU局限于慢性GU	慢性GU研究；用于促GU愈合药物评估；溃疡愈合相关机制研究	通过乙酸灌胃模拟寒邪或热邪侵袭

方法 Methods	优点 Advantages	缺点 Disadvantages	适用范围 Range of application	病理 Pathology
幽门结扎法 Pyloric ligation method	GU重复性好，实验结果稳定；实验造成的GU部位明确，溃疡程度可控	人为结扎为非生理性GU；实验操作技术要求高	急性GU研究；用于抗GU药物评估；GU相关机制研究	通过胃酸堆积、压迫血管，模拟GU
水浸-束缚应激法 Water immersion-restraint stress method	GU复制率高，重复性好；实验操作简单；能较好模拟应激引发的GU	操作过程缺乏量化标准；可能引发其他生理变化；个体差异影响较大；仅适用于短期研究	应激性GU研究；用于抗应激性GU药物评估；应激反应机制研究	通过束缚、水浸，模拟情志失调模型
乙醇诱导法 Ethanol-induced method	操作方法简单，重复性好，成模效果好；能短时间诱导胃黏膜损伤；GU溃疡程度可控	直接损伤胃黏膜为非生理性溃疡；不适用于长期慢性GU研究；死亡率较高	急性GU研究；用于抗GU、胃黏膜保护药物评估；胃黏膜屏障功能机制研究	通过乙醇灌胃，模拟寒邪或热邪侵袭
乙酸诱导法 Acetic acid-induced method	GU形成后持续时间长，重复性好；造模方法简单；溃疡程度可控	直接损伤胃黏膜为非生理性溃疡；诱导GU局限于慢性GU	慢性GU研究；用于促GU愈合药物评估；溃疡愈合相关机制研究	通过乙酸灌胃模拟寒邪或热邪侵袭

方法 Methods	优点 Advantages	缺点 Disadvantages	适用范围 Range of application	病因病机 Pathogenesis
饮食失节法 Eating disorder method	符合中医病因病机理论；造模方法简单；实验结果稳定	建模周期较长；可能引发其他生理变化；个体差异影响较大	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过脾胃功能失调，消化系统紊乱，引发疾病
苦寒泻下法 Bitter-cold purging method	造模方法简单；建模周期较短；模型严重程度可控；标准化程度高；模型可通过药物反证验证	病理因素单一，难以模拟自然多因素病情；可能引起其他生理变化；个体差异影响较大；模型局限于短期研究	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过药物使脾胃阳气受损，引发脾胃衰虚，导致腹痛泻下
过度劳倦法 Excessive fatigue method	可控制劳动程度及劳动时长；可重复性强；病理相关指标关联性强	需精准控制运动强度和精神刺激；模型指标难以量化；可能引发全身性应激反应	中医证候研究；慢性疲劳综合研究；情志致病理论研究；脾胃消化、免疫相关机制研究	通过过度劳倦使气血耗伤，脏腑功能失调，引发脾胃虚寒
耗气破气法 Qi consumption and Qi impairment method	符合中医病因病机理论；实验条件简单，周期较短，易标准化；模型症状与临床症状吻合度高	造成脾胃虚寒程度较轻；可能引起其他生理变化	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过损耗机体正气，使气机失调，破气泄泻，引发脾胃虚寒
过食酸味法 Overeating sour-flavor method	符合中医病因病机理论；操作简单，成本较低；实验贴近真实病理过程，模型与临床症状吻合度高	模型稳定性差，模型指标难以量化；可能引起其他生理变化	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过过度食用酸味食物和药物，使脏腑功能失调，损伤脾胃，导致脾胃虚寒

方法 Methods	优点 Advantages	缺点 Disadvantages	适用范围 Range of application	病因病机 Pathogenesis
饮食失节法 Eating disorder method	符合中医病因病机理论；造模方法简单；实验结果稳定	建模周期较长；可能引发其他生理变化；个体差异影响较大	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过脾胃功能失调，消化系统紊乱，引发疾病
苦寒泻下法 Bitter-cold purging method	造模方法简单；建模周期较短；模型严重程度可控；标准化程度高；模型可通过药物反证验证	病理因素单一，难以模拟自然多因素病情；可能引起其他生理变化；个体差异影响较大；模型局限于短期研究	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过药物使脾胃阳气受损，引发脾胃衰虚，导致腹痛泻下
过度劳倦法 Excessive fatigue method	可控制劳动程度及劳动时长；可重复性强；病理相关指标关联性强	需精准控制运动强度和精神刺激；模型指标难以量化；可能引发全身性应激反应	中医证候研究；慢性疲劳综合研究；情志致病理论研究；脾胃消化、免疫相关机制研究	通过过度劳倦使气血耗伤，脏腑功能失调，引发脾胃虚寒
耗气破气法 Qi consumption and Qi impairment method	符合中医病因病机理论；实验条件简单，周期较短，易标准化；模型症状与临床症状吻合度高	造成脾胃虚寒程度较轻；可能引起其他生理变化	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过损耗机体正气，使气机失调，破气泄泻，引发脾胃虚寒
过食酸味法 Overeating sour-flavor method	符合中医病因病机理论；操作简单，成本较低；实验贴近真实病理过程，模型与临床症状吻合度高	模型稳定性差，模型指标难以量化；可能引起其他生理变化	中医证候研究；用于中药疗效的研发与评估；脾胃消化相关机制研究	通过过度食用酸味食物和药物，使脏腑功能失调，损伤脾胃，导致脾胃虚寒

造模方法 Modeling methods	具体方法 Experimental methods	动物 Animals	参考文献 References	优点 Advantages	缺点 Disadvantages
双因素造模 Two-factor modeling	过食酸味+乙醇	SD大鼠	[41]	操作简单；病理特征明显；成本较低	病理机制单一；稳定性较差；仅适用于初步研究
	苦寒泻下+冰醋酸	SD大鼠	[43]
	苦寒泻下+冰乙酸	SD大鼠	[44]
三因素造模 Three-factor modeling	苦寒泻下+劳倦过度+乙醇、阿司匹林	SD大鼠	[9]	病理机制全面；稳定性和可重复性高；更贴合实际临床；适用于深入研究	实验操作复杂，实验周期长；成本较高；多种因素叠加动物死亡率高
	饮食失节+苦寒泻下+冰乙酸	Wistar大鼠	[29]
	耗气破气+饮食失节+冰醋酸	成年大鼠	[36]
	饮食失节+过食酸味+醋酸涂抹	Wistar大鼠	[40]

脾胃虚寒型胃溃疡动物模型研究进展

Research Progress on Animal Models of Gastric Ulcer of Spleen-Stomach Deficiency Cold Type

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 56

相关文章 15

编辑推荐

Metrics

本文评价

症状体征 Symptoms and signs	观测指标 Observation indicators	变化趋势 Variation trends	参考文献 References
外观症状 Appearance symptoms	皮毛色泽	疏散枯槁	[9,34]
	鼻唇、耳廓、趾爪颜色	鼻唇紫暗，耳廓淡白，趾爪不收	[9]
	舌象	舌质青紫或紫暗，晦暗无光	[20]
动物行为 Animal behavior	精神状态	精神萎靡	[38]
	自主活动	活动缓慢倦怠扎堆，喜弓背，懒动	[9,34]
	寒热趋向	畏寒怕冻，喜扎堆	[9]
代谢指标 Metabolic indicators	饮水量	减少	[9]
	饮食量	减少	[9]
	体重	下降或增长缓慢	[38]
	肛温	下降	[9]
	粪便质地	大便稀软，溏泻	[34]

指标种类 Types of indicators	具体指标 Specific indicators	变化趋势 Variation trends	参考文献 References
胃黏膜溃疡 Gastric mucosa ulcers	胃组织HE染色	胃黏膜上皮有破损，皱襞有中断，上皮组织有点状溃疡及糜烂；黏膜肌层可见水肿、缺损，有明显炎症细胞浸润	[9,36,38]
胃黏膜溃疡 Gastric mucosa ulcers	溃疡指数	升高	[36-37]
胃液 Gastric juice	胃液酸pH 胃蛋白酶活性	胃液总酸度升高胃蛋白酶活性升高	[34] [34,54]
胃状态基础指标 Basic indicators of gastric status	胃泌素	降低	[50]
	胃动素	降低	[41]
	生长抑素	显著下降	[50]
氧化应激指标 Oxidative stress indices	超氧化物歧化酶	降低	[54]
	丙二醛	升高	[9]
	还原型谷胱甘肽	显著下降	[54]
炎性因子 Inflammatory factors	髓样分化因子88	升高	[9,34]
	Toll样受体2	升高	[9,34]
	肿瘤坏死因子-α	升高	[34,38]
	白细胞介素-6	升高	[38]
	白细胞介素-1β	升高	[38]
其他细胞因子 Other cytokines	环氧合酶-2	显著增加	[29]
	前列腺素E2	降低	[9]
	一氧化氮	降低	[9,34]
	磷脂酰肌醇3激酶	显著降低	[38]
	胃黏膜表皮生长因子受体	降低	[38]
	肝细胞生长因子受体	显著上升	[38]
	肝细胞生长因子	显著上升	[38]
凝血4项 Four coagulation parameters	活化部分凝血活酶时间	有升高趋势	[41]
	凝血酶原时间	有升高趋势	[41]
	凝血酶时间	显著延长	[41]
	纤维蛋白原	显著下降	[41]
肠道菌群检测 Intestinal flora detection	拟杆菌门	相对丰度显著降低	[41]
	厚壁菌门	相对丰度有升高趋势	[41]
	变形菌门	相对丰度显著升高	[41]
	联合乳酸杆菌属	相对丰度显著降低	[41]
	罗姆布茨菌属	相对丰度显著增加	[41]

[1]	王笑铭, 孟晨晨, 范鹿, 李艳阳, 张军平, 吕仕超. 中医证候类动物模型构建方法概述[J]. 实验动物与比较医学, 2025, 45(5): 596-610.
[2]	高超奇, 祝志波, 孙显东. 大鼠血管重构模型的应用进展与分类分析[J]. 实验动物与比较医学, 2025, 45(5): 542-550.
[3]	刘洋, 程来洋, 郭中坤. 中医病症结合的围绝经期综合征动物模型研究进展[J]. 实验动物与比较医学, 2025, 45(5): 586-595.
[4]	中国研究型医院学会医学动物实验专家委员会, 中国研究型医院学会神经再生与组织器官损伤修复专业委员会, 中国解剖学会工程解剖学分会, 李忠海, 李斌, 赵杰, 杨操, 李英俊. 椎间盘退行性病变的临床前研究动物模型选择指南（2025年版）[J]. 实验动物与比较医学, 2025, 45(5): 524-541.
[5]	赵鑫, 王晨曦, 石文清, 娄月芬. 斑马鱼在炎症性肠病机制及药物研究中的应用进展[J]. 实验动物与比较医学, 2025, 45(4): 422-431.
[6]	刘亚益, 贾云凤, 左一鸣, 张军平, 吕仕超. 心气阴两虚证动物模型的构建方法与评价进展[J]. 实验动物与比较医学, 2025, 45(4): 411-421.
[7]	潘颐聪, 蒋汶洪, 胡明, 覃晓. 慢性肾脏病大鼠主动脉钙化模型的术式优化及效果评价[J]. 实验动物与比较医学, 2025, 45(3): 279-289.
[8]	李会萍, 高洪彬, 温金银, 杨锦淳. 疾病动物模型数字化图谱数据库平台的构建与初步应用[J]. 实验动物与比较医学, 2025, 45(3): 300-308.
[9]	王碧莹, 鲁家铄, 昝桂影, 陈若松, 柴景蕊, 刘景根, 王瑜珺. 啮齿类动物药物成瘾模型的构建方法和应用进展[J]. 实验动物与比较医学, 2025, 45(2): 158-166.
[10]	陈钰涵, 陈瑾玲, 李欣, 区燕华, 王斯, 陈镜伊, 王兴易, 袁嘉丽, 段媛媛, 羊忠山, 牛海涛. 基于中西医临床病证特点的重症肌无力动物模型分析[J]. 实验动物与比较医学, 2025, 45(2): 176-186.
[11]	连辉, 姜艳玲, 刘佳, 张玉立, 谢伟, 薛晓鸥, 李健. 异常子宫出血大鼠模型的构建与评价[J]. 实验动物与比较医学, 2025, 45(2): 130-146.
[12]	罗世雄, 张赛, 陈慧. 常见哮喘动物模型的建立方法与评价研究进展[J]. 实验动物与比较医学, 2025, 45(2): 167-175.
[13]	费彬, 郭文科, 郭建平. 疝疾病动物模型研究及新型疝修补材料应用进展[J]. 实验动物与比较医学, 2025, 45(1): 55-66.
[14]	杨家豪, 丁纯蕾, 钱风华, 孙旗, 姜旭升, 陈雯, 沈梦雯. 脓毒症相关脏器损伤动物模型研究进展[J]. 实验动物与比较医学, 2024, 44(6): 636-644.
[15]	孙效容, 苏丹, 贵文娟, 陈玥. 手术诱导大鼠中重度膝骨关节炎模型的建立与评价[J]. 实验动物与比较医学, 2024, 44(6): 597-604.