心率变异性分析评估不同因素致亚健康大鼠自主神经功能的影响

doi:10.3969/j.issn.1674-5817.2012.06.002

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (6): 467-472.DOI: 10.3969/j.issn.1674-5817.2012.06.002

• 华东地区第12届实验动物科学学术交流会优秀论文选刊 • 上一篇下一篇

心率变异性分析评估不同因素致亚健康大鼠自主神经功能的影响

潘永明, 刘瑞敏, 吴蔚, 王辉, 贾临超, 徐孝平, 朱科燕, 陈民利

浙江中医药大学动物实验研究中心/比较医学研究中心, 杭州 310053

收稿日期:2012-01-31 出版日期:2012-12-25 发布日期:2012-12-25
作者简介:潘永明(1979-),男,助理研究员,研究方向:实验动物与比较医学。E-mail:pym918@126.com
基金资助:
1.浙江省中医“治未病”研究专项(2007WB022);2.“浙江省卫生高层次创新人才培养工程项目”资助

Assessment of Autonomic Nervous Function in Sub-health Rats Induced by Different Factors Based on Heart Rate Variability Analysis

PAN Yong-ming, LIU Rui-min, Wu Wei, WANG Hui, JIA Lin-chao, XU Xiao-ping, ZHU Ke-yan, CHEN Min-li

Zhejiang Chinese Medical University Laboratory Animal Research Center/Comparative Medical Research Center, Hangzhou 310053, China

Received:2012-01-31 Online:2012-12-25 Published:2012-12-25

摘要/Abstract

摘要： 目的基于心率变异性(heart rate variability, HRV)分析评估不同因素致亚健康大鼠自主神经功能的影响,确定HRV指标与亚健康状态的发生关系。方法将42只雄性SPF级Wistar大鼠,按体质量随机分成7组,即多因素组、多因素干预组、热水游泳组、饮食限制组、睡眠不足组、单纯束缚组和正常对照组,每组6只,通过热水游泳、饮食限制、睡眠剥夺、束缚等方式建立亚健康大鼠模型,多因素干预组给予300 mg/kg的抗衰老片浸膏粉,并分别在造模结束后及恢复3 d时遥测大鼠的心电图,利用HRV分析评估不同因素致亚健康大鼠自主神经功能情况。结果与正常对照组比,时域分析显示亚健康大鼠的SDNN、RMSDD、STV、LTV均有所升高,且频谱分析显示亚健康大鼠VLF、LF、TP、LF/HF、LFnu上升和HFnu下降,且给予抗衰老片后能明显降低LF、LF/HF和LFnu(P<0.05)和显著升高HFnu(P<0.05), 单因素logistic回归分析显示RRI、SDNN、LTV、LF、TP、LF/HF、LFnu、HFnu与亚健康产生密切相关(P<0.05), 且经逐步logistic 回归分析结果显示低下的HFnu(OR值=0.566,P=0.023)可作为评价亚健康产生的独立危险因子。结论不同因素致亚健康大鼠的自主神经功能均出现明显的紊乱, 且迷走神经活动抑制是亚健康产生的独立危险因素, 可见基于HRV分析技术可无创性的客观评估机体亚健康状态的程度。

关键词: 心率变异性, 亚健康, 大鼠, 自主神经功能, 抗衰老片

Abstract: Objective To assess autonomic nervous function in sub-health rats induced by different factors based on Heart Rate Variability (HRV) analysis, and determine the relationship between HRV indexes and sub-health state. Methods Fourty-two male SPF Wistar rats were randomly divided into seven groups: multi-factor group (MF), multifactor treatment group (MFT), warm swimming group (WS), dietary restriction group (DR), sleep deficiency group (SD), purely constraint group (PC) and control group (C), six animals in each group. Using warm swimming, dietary restrictions, sleep deprivation and constraint to establish sub-health rat model. Rats were daily orally administered 300 mg/kg anti-aging tablet in MFT group. The ECG was monitored at the end of the modeling and recovery of 3 days, and HRV analysis has been used to investigate the autonomic nervous function in sub-health rats induced by different factors. Results Compared with control group, standard deviation of the mean R-R interval (SDNN), the root-mean-square of successive differences (RMSSD), short term variability (STV) and long term variability (LTV) parameters of their HRV time domain analysis in sub-health rats were significantly higher than those of control group, and very low frequency power (VLF), low frequency power (LF), total power (TP), LF/HF ratio, normalized low frequency (LFnu) parameters of their HRV frequency domain analysis were increased, while normalized high frequency (HFnu) was decreased. Anti-aging tablets treatment could significantly reduce the LF, LF/HF ratio and LFnu (P<0.05) and increase HFnu (P<0.05). Univariate logistic regression analysis showed that the closely relationship between RRI, SDNN, LTV, LF, TP, LF/HF ratio, LFnu, HFnu and sub-health state (P<0.05), and stepwise logistic regression analysis showed that the low HFnu (OR, value=0.566, P=0.023) maybe independent risk factors for inducing sub-health. Conclusion The autonomic nervous function in sub-health rats induced by different factors was apparent disorder, and inhibition of vagus nerve activity maybe an independent risk factor for sub-health. So HRV analysis could assess the sub-health state.

Key words: Heart rate variability, Sub-health, Rat, Autonomic nervous function, Anti-aging tablet

中图分类号:

Q95-33

潘永明, 刘瑞敏, 吴蔚, 王辉, 贾临超, 徐孝平, 朱科燕, 陈民利. 心率变异性分析评估不同因素致亚健康大鼠自主神经功能的影响[J]. 实验动物与比较医学, 2012, 32(6): 467-472.

PAN Yong-ming, LIU Rui-min, Wu Wei, WANG Hui, JIA Lin-chao, XU Xiao-ping, ZHU Ke-yan, CHEN Min-li. Assessment of Autonomic Nervous Function in Sub-health Rats Induced by Different Factors Based on Heart Rate Variability Analysis[J]. Laboratory Animal and Comparative Medicine, 2012, 32(6): 467-472.

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心率变异性分析评估不同因素致亚健康大鼠自主神经功能的影响

Assessment of Autonomic Nervous Function in Sub-health Rats Induced by Different Factors Based on Heart Rate Variability Analysis

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