关键词: 反义核酸, BALB/c小鼠, 血液生化, 基因功能

Abstract: Objective To investigate the effects of four nutrition metabolism related genes(DL、 Tr、Pe、P3) on blood biochemistry index of BALB/c mouse. Methods Antisensenucleic acids of the four genes with microamount quantitate were injected into the lateral cerebral ventricle of the mouse. The experiment was divided into three groups: antisensenucleic acids group, random sequence nucleic acid control group and saline control group. At the fourth day after thrice injection, using the blood serum to determine the blood biochemistry index. Results Comparing with control group, six indexes of DL and Tr group has significant difference respectively, four and three indexes of Pe and P3 group has significant difference respectively. The triglyceride in blood of the three antisensenucleic acids group (DL、Tr、and P3) BALB/c mouse was significantly higher than the control group, especially the cholesterin of Tr antisensenucleic acids group was obviously higher than the control group, and all were beyond the range of normal values. Moreover, the glucose in the blood of the three antisensenucleic acids group (DL、Tr、and P3) was significantly higher than the control group and beyond the range of normal values. Conclusion The Tr genes have a relation with lipid metabolism, and the DL、Tr、 and P3 genes have also a relation with glycometabolism.

Key words: Antisensenucleic acids, BALB/c mouse, Blood biochemistry, Gene function