关键词: 单纯疱疹病毒Ⅱ型(HSV-2), BALB/c小鼠, 病理学分析, 感染途径

Abstract: Objective To compare the pathological changes of BALB/c mice infected with herpes simplex virus type 2 (HSV-2) by different routes. Method BALB/c mice were challenged with HSV-2 by three different ways , namely, through vagina, nasal cavity and cornea. The clinical manifestations of mice and the pathological changes of challenged site and nerve tissue were observed, and the viral load in nerve tissue was detected. Result There was no overt symptom in the nasal cavity of which the survival rate was 27.27% at the end of observation period. The vaginal group showed different degrees of vulvitis and weight loss and other symptoms, and the survival rate of the mice on day 8 was 0. Corneal group mice showed different degrees of keratitis, the survival rate at the end of observation period was still 63.64%. The results of pathological changes showed that there were obvious pathological changes in the brain tissue of nasal group mice. Both the vagina group and the cornea group mice showed significant pathological changes in the offending sites, and the brain and spinal cord of the vagina group mice showed obvious pathological changes. The results of viral load detection in the nerve tissue showed that, compared with control group, the viral load of HSV-2 in the brain and spinal cord of the three experimental groups was significantly increased with the increase of infection time. Conclusions The infection of HSV-2 by three different ways can cause different pathological changes in mice. This study provides a theoretical basis for the study of the pathogenesis of nervous system disease induced by HSV-2 infection.

Key words: Herpes simplex virus type 2(HSV-2), BALB/c mice, Pathological analysis, Infection route