免疫瘦素促进大鼠脂肪沉积和PPARγ/SREBP-1基因表达

doi:10.3969/j.issn.1674-5817.2019.04.006

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (4): 287-293.DOI: 10.3969/j.issn.1674-5817.2019.04.006

免疫瘦素促进大鼠脂肪沉积和PPARγ/SREBP-1基因表达

吴丽红¹, 李洪涛², 管红兵¹, 顾为望³, 黄莉文¹

1.广州医科大学附属口腔医院·广州口腔疾病研究所·口腔医学重点实验室, 广州 510140;
2.广州医科大学附属第一医院广州呼吸健康研究院, 呼吸疾病国家重点实验室·国家呼吸系统疾病临床医学研究中心, 广州 510230;
3.南方医科大学实验动物管理中心暨比较医学研究所, 广州510515

收稿日期:2019-04-07 出版日期:2019-08-25 发布日期:2021-01-31
作者简介:吴丽红(1984-),女,助理研究员,博士,研究方向:人类疾病动物模型。E-mail:wcanhong@163.com
基金资助:
广东省自然科学基金(2018A030310182),广东省科技计划项目(2018B050502012,2017A030303088,2014A030304069,2015A030303016),广州市科技计划(201707010026)

Immunization with Leptin Promotes Fat Accumulation and Up-regulates mRNA Expression of PPARγand SREBP-1

WU Li-hong¹, LI Hong-tao², Guan Hong-bing¹, GU Wei-wang², Huang Li-wen¹

1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease,Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China;
2. State Key Laboratory of Respiratory Diseases, National Clinical Research Center for Respiratory Diseases, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510230, China;
3. Center of Laboratory Animal of Southern Medical University, Guangzhou 510515, China

Received:2019-04-07 Online:2019-08-25 Published:2021-01-31

摘要/Abstract

摘要： 目的研究免疫重组瘦素(Leptin)对大鼠皮下、腹部和肝脏脂肪代谢及相关基因表达水平的影响。方法 (1)利用重组Leptin或钥孔血蓝蛋白(KLH)免疫30只SD大鼠3次,52 d后测量体质量,实施安死术,称量腹部脂肪和肝脏重量; (2)检测大鼠血清中抗Leptin抗体水平和血脂4项指标(总胆固醇、低密度脂蛋白、高密度脂蛋白和甘油三酯)的水平; (3)采集皮下、腹部脂肪制作石蜡切片并进行HE染色,采集肝脏制作冰冻切片并做油红O染色,观察和分析以上组织的病理变化; (4)利用荧光定量PCR方法检测腹部脂肪Leptin、下丘脑磷酸化过氧化酶活化增生受体(PPARγ)和肝脏类固醇调节元件结合蛋白-1(SREBP-1)基因的mRNA表达水平。结果免疫重组Leptin后,大鼠抗体水平、体质量、总胆固醇密度脂蛋白和甘油三酯均显著升高,病理学观察见脂肪在皮下、腹部和肝脏沉积增多,脂肪代谢相关的Leptin、PPARγ和SREBP-1基因mRNA表达水平升高。结论对SD大鼠免疫重组Leptin,可促进脂肪在皮下、腹部和肝脏的蓄积,并能上调脂肪代谢相关的Leptin、PPARγ和SREBP-1基因表达水平。

关键词: 瘦素(Leptin), 脂肪代谢, 高脂血症, 非酒精性脂肪肝, 磷酸化过氧化酶活化增生受体 (PPARγ), 类固醇调节元件结合蛋白-1(SREBP-1)

Abstract: Objectives To assess the effects of intramuscular injection of exogenous leptin on fat deposition and mRNA expression of fat metabolism related genes in subcutaneous, hypothalamus and liver of rat. Methods Thirty female SD rats were injected thrice with leptin intramuscularly. At the end point of this study, the rats were sacrificed, then the body weight, abdomen fat weight and liver weight were measured. The serum were collected and the total cholesterol, high density lipoprotein, low density lipoprotein and triglycerides were detected. Subcutaneous and abdominal fat were blocked,made into paraffin slices and stained with HE. Livers were blocked, made into frozen slices and stained with Oil Red O. Pathological analysis of fat distribution in the subcutaneous, abdominal and liver. The mRNA expression levels of abdominal fat Leptin, hypothalamus peroxisome proliferator-activatedreceptor-gamma (PPARγ) and liver sterol regulatory element binding protein-1(SREBP-1) were qualified by fluorescence quantitative PCR. Results SD rats administered with leptin immunogen displayed significantly increased body weight, total cholesterol, low density lipoprotein, triglycerides and fat deposition in subcutaneous, abdominal and liver. Conclusions Intramuscular injection of exogenous leptin promotes fat accumulation in subcutaneous, abdominal and liver of rat by up-regulating of Leptin, PPARγ and SREBP-1.

Key words: Leptin, Fat metabolism, Hyperlipidemia, Non-alcoholic fatty liver, Peroxisome proliferator- activatedreceptor-gamma (PPARγ), Sterol regulatory element binding protein-1 (SREBP-1)

中图分类号:

Q95-33

吴丽红, 李洪涛, 管红兵, 顾为望, 黄莉文. 免疫瘦素促进大鼠脂肪沉积和PPARγ/SREBP-1基因表达[J]. 实验动物与比较医学, 2019, 39(4): 287-293.

WU Li-hong, LI Hong-tao, Guan Hong-bing, GU Wei-wang, Huang Li-wen. Immunization with Leptin Promotes Fat Accumulation and Up-regulates mRNA Expression of PPARγand SREBP-1[J]. Laboratory Animal and Comparative Medicine, 2019, 39(4): 287-293.

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免疫瘦素促进大鼠脂肪沉积和PPARγ/SREBP-1基因表达

Immunization with Leptin Promotes Fat Accumulation and Up-regulates mRNA Expression of PPARγand SREBP-1

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