B6-Co小鼠眼睑成纤维细胞增殖、凋亡及迁移能力实验研究

doi:10.3969/j.issn.1674-5817.2019.03.007

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (3): 213-219.DOI: 10.3969/j.issn.1674-5817.2019.03.007

B6-Co小鼠眼睑成纤维细胞增殖、凋亡及迁移能力实验研究

景瑾¹, 张海骏^1,2, 杜利莉², 姬桂青², 邵义祥¹

1.南通大学比较医学研究所, 南通 226001;
2.南通大学江苏省神经再生重点实验室, 南通 226001

收稿日期:2018-12-13 出版日期:2019-06-25 发布日期:2021-01-29
作者简介:景瑾(1991-),女,硕士,助理实验师,研究方向:人类疾病动物模型。E-mail:15051277939@163.com
基金资助:
江苏省自然科学基金(编号:BK2010279);江苏省研究生科研创新计划(编号:KYCX17-1909)

Experimental Study on Proliferation, Apoptosis and Migration of Eyelid Fibroblasts in B6-Co Mice

JING Jin¹, ZHANG Hai-jun^1,2, DU Li-li², JI Gui-qing², SHAO Yi-xiang¹

1. Research Institute of Comparative Medicine of Nantong University, Nantong 226001, China;
2. Jiangsu Key Laboratory of Neuroregeneration of Nantong University, Nantong 226001, China

Received:2018-12-13 Online:2019-06-25 Published:2021-01-29

摘要/Abstract

摘要： 目的探讨B6-Co小鼠出生时眼睑闭合不全的形成机制。方法分离、培养并鉴定B6-Co小鼠及其背景品系C57BL/6J(B6)眼睑成纤维细胞,CCK8法检测B6-Co、 B6小鼠眼睑成纤维细胞的增殖水平,Annexin V-FITC法检测B6-Co、B6小鼠眼睑成纤维细胞的凋亡水平,Transwell检测B6-Co、B6小鼠眼睑成纤维细胞的迁移能力。结果成功培养出B6-Co、B6小鼠眼睑成纤维细胞,B6-Co小鼠眼睑成纤维细胞的增殖能力和迁移能力极显著低于B6小鼠(P<0.01),B6-Co小鼠眼睑成纤维细胞的凋亡水平极显著高于B6小鼠(P<0.01)。结论 B6-Co小鼠眼睑成纤维细胞的增殖能力、凋亡水平及迁移能力均与B6小鼠有极显著差异。

关键词: B6-Co小鼠, 成纤维细胞, 细胞增殖, 细胞凋亡, 细胞迁移

Abstract: Objective To explore the mechanism of eyelid incomplete closure at birth in C57BL/6J-corneal opacity (B6-Co) mice. Methods The eyelid fibroblasts of B6 and B6-Co mice were isolated, cultured and identified. CCK8 method was used to detect the proliferation of eyelid fibroblasts in B6 and B6-Co mice. Apoptosis of eyelid fibroblasts in B6 and B6-Co mice was detected by Annexin V-FITC. Transwell was used to detect the migration ability of eyelid fibroblasts in B6 and B6-Co mice. Results The eyelid fibroblasts of B6 and B6-Co mice were successfully cultured. The proliferation and migration of eyelid fibroblasts from B6-Co mice were significantly lower than those of B6 mice(P<0.01). The apoptosis of eyelid fibroblasts in B6-Co mice was significantly higher than that of B6 mice (P<0.01). Conclusion The proliferative capacity, apoptosis level and migration ability of eyelid fibroblasts in B6-Co mice were significantly different from those of B6 mice.

Key words: B6-Co mouse, Fibroblast, Cell proliferation, Cell apoptosis, Cell migration

中图分类号:

Q95-33

景瑾, 张海骏, 杜利莉, 姬桂青, 邵义祥. B6-Co小鼠眼睑成纤维细胞增殖、凋亡及迁移能力实验研究[J]. 实验动物与比较医学, 2019, 39(3): 213-219.

JING Jin, ZHANG Hai-jun, DU Li-li, JI Gui-qing, SHAO Yi-xiang. Experimental Study on Proliferation, Apoptosis and Migration of Eyelid Fibroblasts in B6-Co Mice[J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 213-219.

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B6-Co小鼠眼睑成纤维细胞增殖、凋亡及迁移能力实验研究

Experimental Study on Proliferation, Apoptosis and Migration of Eyelid Fibroblasts in B6-Co Mice

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