纳米脂质体槲皮素对脑缺血再灌注损伤的神经保护作用及机制

doi:10.3969/j.issn.1674-5817.2020.02.005

摘要/Abstract

摘要： 目的研究纳米脂质体槲皮素(nLQ)对大鼠局灶性脑缺血再灌注损伤的神经保护作用及机制。方法 80只雄性SD大鼠随机分为假手术组、模型组,以及nLQ低、中、高剂量组,每组16只。用改良线栓法制备大鼠中动脉脑缺血再灌注模型。各组大鼠脑缺血再灌注48 h后,采用神经功能缺损评分法观察大鼠的行为学评分; 2,3,5-氯化三苯基四氮唑 (TTC)染色实验测定脑梗死体积,干湿重法测定脑组织含水量;酶联免疫吸附法(ELISA)测定血清中肿瘤坏死因子-α(TNF-α)和白细胞介素10 (IL-10)含量;流式细胞术检测脑组织神经细胞凋亡情况;蛋白质印迹法(Western blotting)检测B淋巴细胞瘤-2基因(Bcl-2)和Bcl-2相关X蛋白(Bax)蛋白表达变化。结果与模型组相比,nLQ低、中、高剂量组大鼠神经功能缺损症状明显改善,行为学评分显著降低,脑梗塞体积显著减小,脑水肿程度显著降低,血清TNF-α和 IL-10的含量显著降低,细胞凋亡率显著降低,凋亡相关蛋白Bcl-2表达水平显著升高,Bax表达水平显著降低(P<0.05或P<0.01),且具有剂量-效应关系。结论 nLQ对大鼠局灶性脑缺血再灌注损伤有很好的保护作用,其机制可能与nLQ能够改善神经功能缺损症状,减少神经细胞凋亡及降低血中炎性反应因子TNF-α和 IL-10的含量有关。

关键词: 纳米脂质体槲皮素, 脑缺血再灌注损伤, 细胞凋亡, 肿瘤坏死因子-α, 白细胞介素10

Abstract: Objective To study the neuroprotective effects and mechanism of nanoliposomal quercetin (nLQ) on focal cerebral ischemia-reperfusion injury in rats. Methods Eighty male SD rats were randomly divided into sham-operation group, model group, nLQ low-dose group, nLQ middle-dose group and nLQ high-dose group, with 16 rats in each group. The middle cerebral artery occlusion model was established by modified suture method. Fourty-eight hours after cerebral ischemia-reperfusion, the behavioral score was observed by using neurological deficit score method, the cerebral infarction volume was measured by TTC staining test, the water content of brain tissue was measured by wet and dry weight method, the contents of tumor necrosis factor α (TNF-α) and interleukin-10 (IL-10) in serum were determined by ELASA assay, the neuronal apoptosis was detected by flow cytometry, and the expressions of Bcl-2 and Bax in brain tissue were detected by Western blotting. Results Compared with the model group rats, nLQ low, middle and high dose groups could significantly improve the neurological deficit symptoms, reduce the behavioral score, decrease the cerebral infarction volume and alleviate cerebral edema, reduce the contents of TNF-α and IL-10 in serum, reduce the rate of neuronal apoptosis, decrease Bax protein expression and increase Bcl-2 protein expression in brain tissue. The differences were statistically significant (P<0.05 or P<0.01), also with a dose-dependent manner. Conclusion The nLQ has a significant protective effect on the focal cerebral ischemia-reperfusion injury in rats. The nLQ may improve the neurological function, reduce the apoptosis of neuronal cells and decrease the content of inflammatory factors TNF- and IL-10 in blood.

Key words: Nanoliposomal quercetin, Cerebral ischemia-reperfusion injury, Apoptosis, Tumor necrosis factor α, Interleukin 10

中图分类号:

Q95-33

康瑜, 杨小芳. 纳米脂质体槲皮素对脑缺血再灌注损伤的神经保护作用及机制[J]. 实验动物与比较医学, 2020, 40(2): 116-122.

KANG Yu, YANG Xiaofang. Protective Effect and Mechanism of Nanoliposomal Quercetin on Focal Cerebral Ischemia-reperfusion Injury in Rats[J]. Laboratory Animal and Comparative Medicine, 2020, 40(2): 116-122.

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