兔VX2肿瘤模型的研究进展

doi:10.3969/j.issn.1674-5817.2019.02.017

摘要/Abstract

摘要： 兔VX2肿瘤模型是目前比较常用的建立在较大动物身上的移植瘤模型,因其体型大、容易复制,在外科手术、影像学及介入治疗等研究中广泛应用。VX2肿瘤模型的建立方法包括肿瘤细胞悬液接种和肿瘤组织块移植接种,根据移植部位的不同可以建立多种类型的肿瘤模型,研究人员可以根据自己的研究目的选用合适的类型。随着影像设备的普及和新兴局部治疗方法的应用,采用兔VX2肿瘤模型的研究越来越多。本文从VX2细胞的来源及生物学特性、兔VX2肿瘤模型的建立方法及类型、研究应用等方面进行综述。

关键词: 兔, VX2, 肿瘤, 动物模型

Abstract: The rabbit VX2 tumor model is commonly used as a xenograft tumor model in large animals, which is also widely used in surgical operation, imageology and interventional therapy because of its body type and easy replication. The methods of establishing rabbit VX2 tumor model include injection of cell suspension and inoculation of tumor tissue block. The tumor models can be established differently depending on the site of the transplant. Researchers can select suitable tumor models for their own purposes. With the popularity of imaging equipment and the application of the emerging local treatment methods, more and more studies have been conducted using the rabbit VX2 tumor model. This paper reviewed the source of VX2 cells, biological characteristics, methods of tumor models and research applications.

Key words: Rabbit, VX2, Tumor, Model

中图分类号:

Q95-33

米金霞, 方肇勤. 兔VX2肿瘤模型的研究进展[J]. 实验动物与比较医学, 2019, 39(2): 163-168.

MI Jin-xia, FANG Zhao-qin. Research Progress of Rabbit VX2 Tumor Model[J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 163-168.

参考文献

[1] Chen W, Zheng R, Baade PD, et al.Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2):115-132.
[2] Gehlot P, Shukla V, Gupta S, et al.Detection of ALDH1 activity in rabbit hepatic VX2 tumors and isolation of ALDH1 positive cancer stem cells[J]. J Transl Med, 2016, 14:49.
[3] Rous P, Beard JW.The progression to carcinoma of virus-induced rabbit papillomas (Shope)[J]. J Exp Med, 1935, 62: 523-548.
[4] Kidd JG, Rous P.A transplantable rabbit carcinoma originating in a virus-induced papilloma and containing the virus in masked or altered form[J]. J Exp Med, 1940, 71:813-838.
[5] 刘险峰, 任乐荣, 苏广彦, 等. 兔VX2肿瘤细胞系的建立及其生物学特性的观察[J]. 中华病理学杂志 , 2005, 34(10): 661-663.
[6] 苏畅, 张惠中, 李文海, 等. 兔VX2肿瘤的离体培养及有关生物学特性[J]. 第四军医大学学报, 2006(9):844-847.
[7] 赵中辛, 杜竞辉, 王学浩, 等. 毗喃霉素-碘油肝动脉灌注治疗兔VX2瘤的药代动力学研究[J]. 中华实验外科杂志, 1998, 15(3):244-245.
[8] 王光大, 吴勇超, 李亚洲, 等. 兔VX2肝脏转移瘤血供来源分析[J]. 医学动物防制, 2017, 33(3):240-242+248+356.
[9] Li JL, Ye WT, Liu ZY, et al.Comparison of microvascular perfusion evaluation among IVIM-DWI, CT perfusion imaging and histological microvessel density in rabbit liver VX2 tumors[J]. Magn Reson Imaging, 2018, 46:64-69.
[10] Ahn SY, Goo JM, Lee KH, et al.Monitoring tumor response to the vascular disrupting agent CKD-516 in a rabbit VX2 intramuscular tumor model using PET/MRI: Simultaneous evaluation of vascular and metabolic parameters[J]. PLoS One, 2018, 13(2):1-14.
[11] 丁玖乐, 陈蕾, 孙玲玲, 等. VX2瘤在不同CT机型下常规腹部扫描的纹理分析差异及预处理评价[J]. 中国医学影像学杂志, 2018, 26(1):7-10.
[12] 余梁, 含笑, 周丽芬, 等. 兔VX2肝癌模型改良制作及TAE前后CT与DSA影像学评估[J]. 中国CT和MRI杂志, 2017, 15(8):85-87.
[13] 于涛, 魏然, 金宇, 等. 兔VX2骨转移瘤的适宜穿刺活检时间研究[J]. 中华实用诊断与治疗杂志, 2017, 31(6):530-532.
[14] Tong H, Duan LG, Zhou HY, et al.Modification of the method to establish a hepatic VX2 carcinoma model in rabbits[J]. Oncol Lett, 2018, 15(4):5333-5338.
[15] 张贵祥, 孙诚, 魏梦绮, 等. 介绍兔VX2转移瘤动物模型[J]. 实用放射学杂志, 1998, 14(2):59.
[16] Pascale F, Bédouet L, Fazel A, et al.Lymphatic transport and lymph node location of microspheres subcutaneously injected in the vicinity of tumors in a rabbit model of breast cancer[J]. Pharm Res, 2018, 35(10):191.
[17] Zhang H, Xiong F, Qian K, et al.Transcatheter arterial embolization combined with hypoxia-replicative oncolytic adenovirus perfusion enhances the therapeutic effect of hepatic carcinoma[J]. Cancer Manag Res, 2019, 11:981-996.
[18] 孙轶群, 童彤, 毛健, 等. VX2移植性兔直肠癌淋巴结转移动物模型的建立及其生物学特性[J]. 中国癌症杂志, 2016, 26(10):840-847.
[19] 许健东, 方利平. 蜂胶对兔骨骼肌VX2肿瘤生长抑制作用的研究[J]. 临床合理用药杂志, 2016, 9(33):134-136.
[20] 马雅昌, 郭健, 朱雅文, 等. 一项新型封闭穿刺技术应用于骨肿瘤穿刺活检中的价值[J]. 遵义医学院学报, 2018, 41(01):53-57+62.
[21] 含笑, 胡茂能, 吴鸿峰, 等. 建立改良法兔VX2肝癌模型及肝动脉联合门静脉插管的应用[J]. 中国介入影像与治疗学, 2017, 14(11):690-693.
[22] 金光鑫, 王军, 仇晓霞, 等. MR导引经皮穿刺瘤块种植法构建兔VX2肝癌模型[J]. 介入放射学杂志, 2016, 25(11): 980-983.
[23] 张强, 李彬, 李晓光, 等. CT导引下“体外预装示踪一步植入技术”制作兔肝/肾肿瘤模型[J]. 介入放射学杂志, 2018, 27(03):252-256.
[24] Li H, Yu L, Wang W, et al.Dynamics of angiogenesis and cellularity in rabbit VX2 tumors using contrast-enhanced magnetic resonance imaging and diffusion-weighted imaging[J]. Oncol Lett, 2018, 15(3):2978-2984.
[25] 潘江洋, 时高峰, 王琦, 等. DCE-MRI评估索拉非尼抑制兔VX2肝种植瘤血管生成的实验研究[J]. 放射学实践, 2018, 33(1):13-19.
[26] 王攀鸽, 谭红娜, 王博, 等. MR淋巴造影显影兔VX2乳腺癌模型内乳前哨淋巴结[J]. 中国医学影像技术, 2018, 34(5):641-645.
[27] Chen X, Liu HP, Li M, et al.Advances in non-surgical management of primary liver cancer[J]. World J Gastroenterol, 2014, 20(44):16630-16638.
[28] 刘亚, 吕维富, 朱先海, 等. 兔肝VX2肿瘤射频消融后不同区带VEGF的表达和细胞凋亡[J]. 中国介入影像与治疗学, 2017, 14(9):561-565.
[29] 邹孟达, 熊青, 邓青松, 等. 环五肽RGD靶向的纳米金偶联VEGFsiRNA增强兔VX2肝肿瘤射频消融损伤效应的研究[J]. 中国普通外科杂志, 2017, 26(1):43-49.
[30] 赵艳萍, 齐玲俊, 刘文亚, 等. CT评价兔VX2肝癌射频消融后局部肿瘤进展模式及部位[J]. 中国医学影像技术, 2011, 27(3):474-478.
[31] 明韦迪, 李晓光, 金征宇. 兔小腿VX2 肿瘤射频消融前后血清HSP70、IL-6、IL-8水平的变化及意义[J]. 医学影像学杂志, 2017, 27(5):906-910.
[32] Bimonte S, Leongito M, Piccirillo M, et al.Radio-frequency ablation-based studies on VX2 rabbit models for HCC treatment[J]. Infect Agent Cancer, 2016, 11:38.
[33] Jiang T, Zhang X, Ding J, et al.Inflammation and cancer: inhibiting the progression of residual hepatic VX2 carcinoma by anti-inflammation drug after incomplete radiofrequency ablation[J]. Int J Clin Exp Pathol, 2015, 8(11):13945-13956.
[34] 李浩, 段旭华, 韩新巍, 等. 经肝动脉化疗栓塞术联合射频消融治疗兔肝VX2 肿瘤的效果分析[J]. 临床肝胆病杂志, 2018, 34(4):779-783.
[35] Tomozawa Y, Nitta N, Ohta S, et al.Anti-tumor effects of sorafenib administered at different time points in combination with transarterial embolization in a rabbit VX2 liver tumor model[J]. Cardiovasc Intervent Radiol, 2017, 40(11): 1763-1768.
[36] Zhang X, Dai F, Chen J, et al.Antitumor effect of curcumin liposome after transcatheter arterial embolization in VX2 rabbits[J]. Cancer Biol Ther, 2019, 8:1-11.
[37] 黄巧胜, 陈少锋, 钟泽龙, 等. 雷替曲塞经不同给药方式的药动学分析[J]. 介入放射学杂志, 2018, 27(2):157-162.
[38] 刘宇, 张福军, 兰作平, 等. 基于兔VX2原位移植肺癌模型评价多西他赛脂质体疗效和安全性[J]. 第三军医大学学报, 2017, 39(14):1428-1434.
[39] 董胜利, 李高鹏, 宋建, 等. 纳米颗粒介导的P53联合Rb基因靶向治疗对兔VX2肝转移癌多药耐药的影响及分子机制[J]. 中国药物与临床, 2017, 17(4):468-471+618.
[40] 梁琪, 邓灵灵, 冯智超, 等. SPIO纳米药物载体联合碘化油经肝动脉栓塞治疗兔VX2肝癌[J]. 中南大学学报: 医学版, 2017, 42(11):1248-1256.
[41] 戴海涛, 黄勇慧, 林润, 等. 磷酰胆碱修饰的氧化石墨烯经导管动脉化疗栓塞治疗肝癌[J]. 中山大学学报: 医学版, 2018, 39(1):18-25.
[42] Sun Y, Xiong X, Pandya D, et al.Enhancing tissue permeability with MRI guided preclinical focused ultrasound system in rabbit muscle: From normal tissue to VX2 tumor[J]. J Control Release, 2017, 256:1-8.
[43] 陈云卫, 宋晓雪, 吴堃, 等. 腹腔镜下射频凝血器对家兔VX2肝癌模型的疗效研究[J]. 现代医药卫生, 2017, 33(23): 3546-3548.
[44] 宋春慧, 赵伟, 李嫚, 等. 氩氦刀治疗兔VX2肝肿瘤的影像表现与病理分析[J]. 医疗装备, 2017, 30(9):1-3.
[45] Oshiro H.The role of the lymphatic system in rabbit models for cancer metastasis research: a perspective from comparative anatomy[J]. Okajimas Folia Anat Jpn, 2014, 91(2): 25-28.
[46] National Health and Family Planning Commission of the People's Republic of China. Diagnosis, management, and treatment of hepatocellular carcinoma (V2017)[J]. J Clin Hepatol, 2017, 33(8):1419-1431.
[47] Urano M, Tanaka C, Sugiyama Y, et al.Antitumor effects of residual tumor after cryoablation: the combined effect of residual tumor and a protein-bound polysaccharide on multiple liver metastases in a murine model[J]. Cryobiology, 2003, 46:238-245.
[48] Chandra D, Jahangir A, Cornelis F, et al.Cryoablation and Meriva have strong therapeutic effect on triple-negative breast cancer[J]. Oncoimmunology, 2015, 5:e1049802.
[49] 米金霞, 卢涛, 方肇勤, 等. 实体瘤中心变性粉碎引流术在兔VX2移植瘤模型实施的可行性评价[J]. 上海中医药大学学报, 2018, 32(5):41-44.
[50] Huisman F, van Lienden KP, Damude S, et al. A review of animal models for portal vein embolization[J]. J Surg Res, 2014, 191(1):179-188.