饮食中糖分控制对雌黑腹果蝇寿命和中肠干细胞的影响

doi:10.3969/j.issn.1674-5817.2019.02.009

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (2): 118-123.DOI: 10.3969/j.issn.1674-5817.2019.02.009

饮食中糖分控制对雌黑腹果蝇寿命和中肠干细胞的影响

唐润东¹, 宋思远², 吴薇³

1.上海市七宝中学, 上海201100;
2.上海外国语大学附属浦东外国语学校, 上海200120;
3.中国科学院上海生物化学与细胞生物学研究所, 上海200031

收稿日期:2018-12-10 出版日期:2019-04-25 发布日期:2021-01-29
作者简介:唐润东(2000-),男,上海市七宝中学高三学生。E-mail:rundongtang@sina.com共同第一作者:宋思远(2002-),女,上海外国语大学附属浦东外国语学校高二学生。E-mail:oceanesong@163.com

Effects of Sugar Concentration Control on the Longevity and the Mid-gut Stem Cells of female Drosophila Melanogaster

TANG Run-dong¹, SONG Si-yuan², WU Wei³

1. Shanghai Qibao High School, Shanghai 201100, China;
2. Pudong Foreign Languages School, SISU, Shanghai 200120, China;
3. Institute of Bichemistry and Cell Biology, CAS, Shanghai 200031, China

Received:2018-12-10 Online:2019-04-25 Published:2021-01-29

摘要/Abstract

摘要： 目的研究饮食中糖分控制对雌黑腹果蝇寿命和中肠干细胞的影响, 以期给女性树立正确科学的饮食观念提供一定的指导。方法用不同糖分食物喂养雌黑腹果蝇,称体质量,记录存活数目, 解剖中肠进行免疫荧光染色。结果随着年龄的增长, 正常组(以正常培养基含糖量为100%糖分)和高糖组(正常组糖分基础上增加20%糖分)雌黑腹果蝇体质量均呈现先缓慢上升, 21 d后快速上升趋势,高糖组体质量增长高于正常组。高糖喂养后用糖分梯度递减培养基喂养,体质量随着糖分减少呈先下降后上升趋势,体质量增长最慢的是用50%糖分培养基喂养。随着处理时间的延长,雌黑腹果蝇在前14 d基本没有死亡,14 d以后,死亡数量剧增,21 d以后呈直线下降趋势。梯度糖分控制后,雌黑腹果蝇平均寿命有不同程度的增加,高糖喂养后喂食50%糖分培养基寿命增长最明显。喂养28 d的雌黑腹果蝇,高糖组肠道比正常组增粗,细胞形态变化大,排列无序,高糖喂养后用糖分梯度培养基喂养,50%和25%糖分处理组恢复效果较好,但不能恢复到正常饮食组状态。结论糖分对雌黑腹果蝇的体质量、寿命和中肠干细胞形态和分布都有一定的影响。适当减少糖摄入量对果蝇体质量增长有减缓作用,对寿命有增加作用,中肠损伤相对较轻。

关键词: 黑腹果蝇, 体质量, 寿命, 中肠干细胞

Abstract: Objective To assess the effects of sugar concentration control on the longevity and the mid-gut stem cells of female Drosophila Melanogaster and then to find a optimum eating habits for women. Methods Detect the variance of body weight, survival rate, longevity and mid-gut stem cell’s form with gradient sugar concentration treatment (the sugar concentration in normal medium as 100%). Results With the increase of the age, all the fly groups’ body weight raised slowly first and then rapidly after 21 days. Body weight in the high sugar concentration treatment group increased faster than that of low sugar concentration treatment group. With the experiments of gradient sugar concentration treatment after high sugar concentration treatment, the optimum sugar concentration is 50%. With the extended of the treat time, the survival rate increased rapidly after 14 days. All the groups’ survival rates were increasing with the gradient sugar concentration treatment after high sugar concentration treatment group. Drosphila in the 50% sugar concentration treatment group had the highest survival rate. With the sugar concentration control, all the fly groups’ average longevity raised and the optimum sugar concentration was 50%. With the extended of the treat time, all the groups’ mid-gut stem cell had large morphological changes. They had be rescued by the sugar concentration control and the optimum sugar concentration was 50%. Conclusion The sugar was a important nutrient for D.melanogaster. It had large effects on D. melanogaster’s body weight, longevity and mid-gut stem cell. The body weight decreased and the longevity increased with the diet control. And they could rescue the damage on mid-gut stem cell.

Key words: Drosophila Melanogaster, Body weight, Longevity, Mid-gut stem cells

中图分类号:

Q95-33

唐润东, 宋思远, 吴薇. 饮食中糖分控制对雌黑腹果蝇寿命和中肠干细胞的影响[J]. 实验动物与比较医学, 2019, 39(2): 118-123.

TANG Run-dong, SONG Si-yuan, WU Wei. Effects of Sugar Concentration Control on the Longevity and the Mid-gut Stem Cells of female Drosophila Melanogaster[J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 118-123.

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饮食中糖分控制对雌黑腹果蝇寿命和中肠干细胞的影响

Effects of Sugar Concentration Control on the Longevity and the Mid-gut Stem Cells of female Drosophila Melanogaster

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