人参皂甙Rg1慢性阻塞性肺疾病大鼠肺组织上皮间充质转化的影响

doi:10.3969/j.issn.1674-5817.2018.06.004

实验动物与比较医学 ›› 2018, Vol. 38 ›› Issue (6): 422-427.DOI: 10.3969/j.issn.1674-5817.2018.06.004

人参皂甙Rg1慢性阻塞性肺疾病大鼠肺组织上皮间充质转化的影响

刘乾, 管思彬, 韩锋锋, 郭雪君

上海交通大学医学院附属新华医院,上海 200062

收稿日期:2018-07-04 出版日期:2018-12-25 发布日期:2021-03-01
作者简介:刘乾(1980-),女,医学博士,呼吸科主治医师,从事呼吸系统疾病研究。E-mail:liuqian_1980@hotmail.com
基金资助:
国家青年科学基金项目(81400026)

Ginsenoside Rg1 Alleviated Epithelial Mesenchymal Transition of Airway in Chronic Obstructive Pulmonary Disease Rat

LIU Qian, GUAN Si-bin, HAN Feng-feng, GUO Xue-jun

Xinhua Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200092,China

Received:2018-07-04 Online:2018-12-25 Published:2021-03-01

摘要/Abstract

摘要： 目的通过烟熏法建立慢性阻塞性肺疾病(COPD)大鼠模型,用人参皂甙Rg1灌胃,研究人参皂甙Rg1对小气道上皮细胞上皮间充质转化(EMT)的影响。方法烟熏诱导大鼠COPD模型,观察肺功能变化,肺组织HE染色检测大鼠肺气肿和小气道破坏情况。免疫组织化学、实时PCR检测肺组织E-cad和α-SMA的表达,观察人参皂甙Rg1对肺内EMT的影响。结果人参皂甙Rg1可以减轻烟熏对大鼠肺结构破坏,改善肺功能,降低肺内EMT。结论人参皂甙Rg1可能通过抑制EMT来改善COPD大鼠小气道纤维化。

关键词: 慢性阻塞性肺疾病(COPD), 人参皂甙Rg1, 小气道纤维化, 上皮间充质转化(EMT)

Abstract: Objective To investigate the effect and the related mechanism of ginsenoside Rg1 in epithelial mesenchymal transition (EMT) in chronic obstructive pulmonary disease (COPD). Methods The COPD rat models were induced by exposure to cigarette smoke. Ginsenoside Rg1 was oral gavage dosed. The pulmonary function test and lung histologic HE staining were used to evaluate emphysema and small airway destruction. The expressions of E-cad and α-SMA were measured using immunohistochemistry assay, real-time PCR to evaluate the effect of ginsenoside Rg1 on EMT. Results Ginsenoside Rg1 ameliorated emphysema and airway destruction in COPD rats, improved pulmonary function, alleviated airway EMT. Conclusion Ginsenoside Rg1 can protest EMT in lung tissue,and may be act as potent intervention against small airway fibrosis in COPD.

Key words: Chronic obstructive pulmonary disease (COPD), Ginsenoside Rg1, Small airway fibrosis, Epithelial mensenchymal transition (EMT)

中图分类号:

Q95-33

刘乾, 管思彬, 韩锋锋, 郭雪君. 人参皂甙Rg1慢性阻塞性肺疾病大鼠肺组织上皮间充质转化的影响[J]. 实验动物与比较医学, 2018, 38(6): 422-427.

LIU Qian, GUAN Si-bin, HAN Feng-feng, GUO Xue-jun. Ginsenoside Rg1 Alleviated Epithelial Mesenchymal Transition of Airway in Chronic Obstructive Pulmonary Disease Rat[J]. Laboratory Animal and Comparative Medicine, 2018, 38(6): 422-427.

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人参皂甙Rg1慢性阻塞性肺疾病大鼠肺组织上皮间充质转化的影响

Ginsenoside Rg1 Alleviated Epithelial Mesenchymal Transition of Airway in Chronic Obstructive Pulmonary Disease Rat

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