基因重组生长激素对慢性阻塞性肺疾病大鼠营养状况及肺功能影响

doi:10.3969/j.issn.1674-5817.2013.01.011

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (1): 52-55.DOI: 10.3969/j.issn.1674-5817.2013.01.011

基因重组生长激素对慢性阻塞性肺疾病大鼠营养状况及肺功能影响

文文, 柳德灵, 官泽金, 黄欲海, 陈美莲, 叶嘉, 赖国祥

南京军区福州总医院呼吸与危重症医学科, 福州350025

收稿日期:2012-05-04 出版日期:2013-02-25 发布日期:2013-02-25
作者简介:文文(1981-), 女, 硕士, 主治医师。研究方向: 慢性阻塞性肺疾病, E-mail: fjfzfisher@163.com
基金资助:
福建省科技计划重点项目(2009Y0038)

Effect of Recombinant Growth Hormone on Nutritional Status and Pulmonary Function in COPD Rat

WEN Wen, LIU De-ling, GUAN Ze-jin, HUANG Yu-hai, CHEN Mei-lian, YE Jia, LAI Guo-xiang

Department of Pulmonary and Critical Care Medicine, fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025, China

Received:2012-05-04 Online:2013-02-25 Published:2013-02-25

摘要/Abstract

摘要： 目的探讨基因重组生长激素(GH)对慢性阻塞性肺疾病(COPD)大鼠营养状况和肺功能的影响。方法通过吸烟联合脂多糖复制大鼠COPD模型,将16例COPD大鼠随机分成2组, GH组为生长激素治疗组(n=8),每日皮下注射基因重组鼠生长激素1 µg,连续使用7 d。NS组为对照组(n=8),皮下注射等容积的生理盐水。所有大鼠均于治疗后15 d,检测大鼠体质量,血清白蛋白(ALB)、转铁蛋白(TRF)、前清蛋白(PA)、肌酐、尿素氮及血糖,并测定大鼠肺功能。结果所制COPD模型符合COPD的基本病理改变。治疗前两组体质量、白蛋白、前清蛋白、总蛋白、肌酐、尿素氮、血糖差异无显著性(P>0.05),生长激素组体质量、白蛋白、前清蛋白、总蛋白治疗后较对照组差异有显著性提高(P<0.05),肌酐、尿素氮有显著性下降(P<0.05),生长激素组治疗前后及治疗后与生理盐水对照组相比血糖有增高趋势,但差异无统计学意义(P>0.05)。肺功能指标(FEV_0.3,FEV_0.3/FVC,PEF)治疗后较生理盐水对照组明显升高(P<0.05)。结论生长激素治疗能改善稳定期慢性阻塞性肺疾病大鼠营养状态,提高肺功能。

关键词: 基因重组生长激素, 慢性阻塞性肺疾病(COPD), 营养状况, 肺功能

Abstract: Objective To study the effect of gene recombinant growth hormone (GH) on nutritional status and lung function of chronic obstructive pulmonary disease (COPD) rats. Methods The COPD rats was established through smoking combined with lipopolysaccharide, 16 cases of COPD rats were randomly divided into 2 groups, group GH for growth hormone treatment (8 cases), daily subcutaneous injections of recombinant rat growth hormone 1 µg,7 days of continuous use, group NS (8 cases) with subcutaneous injection of physiological saline. Fifteenth days after treatment, the rat body weight, serum albumin (ALB), transferrin (TRF), prealbumin (PA), creatinine, blood urea nitrogen and glucose of all the rats were detected, and the lung function were evaluated. Results The COPD model conforms to the COPD basic pathological changes. In two groups before treatment weight, albumin, prealbumin, total protein, creatinine, blood urea nitrogen, blood glucose had no significant difference (P>0.05), body weight, albumin, prealbumin, total protein of growth hormone group increase has higher than the control group (P<0.05), creatinine, urea nitrogen were significantly decreased (P<0.05), the glucose increased before and after treatment with growth hormone group, but the difference was not statistically significant (P>0.05). Lung function (FEV0.3, FEV0.3/FVC, PEF) after treatment compared with the saline control group were significantly higher (P<0.05). Conclusions The growth hormone therapy can improve nutritional status and lung function of the stable phase of chronic obstructive pulmonary disease rat.

Key words: Recombinant growth hormone, Chronic obstructive pulmonary disease, Nutritional status, Lung function

中图分类号:

Q95-33

文文, 柳德灵, 官泽金, 黄欲海, 陈美莲, 叶嘉, 赖国祥. 基因重组生长激素对慢性阻塞性肺疾病大鼠营养状况及肺功能影响[J]. 实验动物与比较医学, 2013, 33(1): 52-55.

WEN Wen, LIU De-ling, GUAN Ze-jin, HUANG Yu-hai, CHEN Mei-lian, YE Jia, LAI Guo-xiang. Effect of Recombinant Growth Hormone on Nutritional Status and Pulmonary Function in COPD Rat[J]. Laboratory Animal and Comparative Medicine, 2013, 33(1): 52-55.

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基因重组生长激素对慢性阻塞性肺疾病大鼠营养状况及肺功能影响

Effect of Recombinant Growth Hormone on Nutritional Status and Pulmonary Function in COPD Rat

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