淫羊藿素对BALB/c-nu 裸小鼠人源性前列腺癌模型的影响

doi:10.3969/j.issn.1674-5817.2018.06.005

实验动物与比较医学 ›› 2018, Vol. 38 ›› Issue (6): 428-433.DOI: 10.3969/j.issn.1674-5817.2018.06.005

淫羊藿素对BALB/c-nu 裸小鼠人源性前列腺癌模型的影响

宋登鹏¹, 饶红¹, 韩安艳¹, 武福云², 陈德森²

1.十堰市太和医院湖北医药学院附属医院,十堰 442000;
2.湖北医药学院基础医学院,十堰 442000

收稿日期:2018-01-22 出版日期:2018-12-25 发布日期:2021-03-01
作者简介:宋登鹏(1982-),男,研究方向:泌尿系肿瘤治疗药物及临床药理。E-mail:dengpenghb@163.com
基金资助:
国家自然科学基金项目(81702639);2018年十堰市科学技术研究与开发项目计划(18Y12)

Effects of Icariin on Human BALB/c-nu Prostate Cancer Model in Nude Mice

SONG Deng-peng¹, RAO Hong¹, HAN An-yan¹, WU Fu-yun¹, CHEN De-sen

1. Department of Paediatric Pharmacy,Shiyan Taihe Hospital,Hubei University of Medicine,Shiyan,442000,China;
2. Basic Medical College,Hubei University of Medicine,Shiyan 442000,China

Received:2018-01-22 Online:2018-12-25 Published:2021-03-01

摘要/Abstract

摘要： 目的探讨淫羊藿素抑制人源性前列腺癌LNCaP细胞珠BALB/c-nu 裸小鼠荷瘤组织磷脂酰肌醇3-激酶/蛋白激酶B(P13K/Akt) 信号通路及其相关蛋白磷酸化的可能机制。方法将40只雄性BALB/c-nu裸小鼠按随机对照原则均分为4组[对照组、前列腺癌(PCa)组、阳性药组和实验组]并采用前列腺内注射LNCaP细胞株构建PCa动物模型。实验组每日按0.1 mL/10 g尾静脉注射0.8%淫羊藿素,阳性药组按0.1 mL/10 g尾静脉注射P13K/ Akt抑制剂LY294002,对照组和PCa组注射等体积生理盐水。注射4周后比较裸小鼠体质量、前列腺瘤体湿重、体积及P13K/Akt 信号通路相关蛋白表达。结果与PCa组比较,实验组前列腺瘤体湿重、体积、P13K、 p-Akt、磷酸化雄激素受体(p-AR)、雄激素受体剪接变异体7(AR-V7)和降钙素(Calcitonin)均较模型组降低(P<0.05),而钙黏蛋白E(E-cadherin) 升高(P<0.05)。结论淫羊藿素通过调节PI3K/Akt信号通路抑制PCa的进展,且这一作用与Akt及AR磷酸化有关。

关键词: 淫羊藿素, BALB/c-nu裸小鼠, 前列腺癌(PCa), 磷脂酰肌醇3-激酶/蛋白激酶B (P13K/ Akt) 信号通路, 磷酸化

Abstract: Objective To explore the possible mechanism of icariin inhibiting phosphoinositide 3-kinase/protein kinase B (P13K/Akt) signaling pathway and phosphorylation of related proteins in human prostate cancer (LNCaP) BALB/c-nu nude mice. Methods Totally 40 male BALB/c-nu nude mice were randomly divided into 4 groups (control group, prostate cancer group, positive drug group and experimental group) and the human prostate cancer model was established by intraprostate injection of LNCaP. The experimental group was injected 0.8% icariin by 0.1 mL/(10 g·d) tail vein, the positive group was injected 0.1 mL/10 g tail vein P13K/Akt inhibitor LY294002, and control group and the prostate cancer group were injected the same volume of normal saline. The body weight, wet weight, volume and P13K/Akt signaling pathway-related protein expression in nude mice were compared after 4 weeks of injection. Results The wet weight, volume, P13K, P-Akt, P-AR, AR-V7 and Calcitonin of prostate cancer tissues in the experimental group were lower than those in the model group (P<0.05), while E-cadherin was higher (P<0.05). Conclusion Icariin inhibits the progress of LNCaP by regulating PI3K/ Akt signaling pathway, which is related to the phosphorylation of Akt and AR.

Key words: Icariin, BALB/c-nu nude mice, Human prostate cancer/model, Phosphoinositide 3-kinase/ protein kinase B (P13K/Akt) signaling pathway, Phosphorylation

中图分类号:

Q95-33

宋登鹏, 饶红, 韩安艳, 武福云, 陈德森. 淫羊藿素对BALB/c-nu 裸小鼠人源性前列腺癌模型的影响[J]. 实验动物与比较医学, 2018, 38(6): 428-433.

SONG Deng-peng, RAO Hong, HAN An-yan, WU Fu-yun, CHEN De-sen. Effects of Icariin on Human BALB/c-nu Prostate Cancer Model in Nude Mice[J]. Laboratory Animal and Comparative Medicine, 2018, 38(6): 428-433.

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淫羊藿素对BALB/c-nu 裸小鼠人源性前列腺癌模型的影响

Effects of Icariin on Human BALB/c-nu Prostate Cancer Model in Nude Mice

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