应用微卫星DNA标记对福建亚种猕猴遗传多样性的分析

doi:10.3969/j.issn.1674-5817.2017.06.003

实验动物与比较医学 ›› 2017, Vol. 37 ›› Issue (6): 434-441.DOI: 10.3969/j.issn.1674-5817.2017.06.003

应用微卫星DNA标记对福建亚种猕猴遗传多样性的分析

周建华¹, 李志雄², 杨燕燕¹, 谢金东¹, 俞春英¹, 王训立¹

1.福建中医药大学实验动物中心, 福州 350122;
2.福建省计划生育科学技术研究所, 福州 350011

收稿日期:2017-04-27 出版日期:2017-12-25 发布日期:2017-12-25
作者简介:周建华(1969-),男,硕士,高级实验师,研究方向:实验动物学。E-mail:zjh001866@sina.com
基金资助:
福建省实验动物研究重点项目(2014Y0080)

Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers

ZHOU Jian-hua¹, LI Zhi-xiong², YANG Yan-yan¹, XIE Jin-dong¹, YU Chun-ying¹, WANG Xun-li¹

1. Laboratory Animal Center, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;
2. Fujian Research Institute of Family Planning, Fuzhou 350011, China

Received:2017-04-27 Online:2017-12-25 Published:2017-12-25

摘要/Abstract

摘要： 目的研究福建亚种猕猴的遗传多样性水平,为猕猴遗传质量监测方法提供基础资料。方法采用20个微卫星DNA(SSR)标记技术,经基因组DNA提取、PCR扩增、非变性聚丙烯酰胺凝胶电泳等,对28只福建亚种猕猴遗传多样性进行分析,用POPGENE 1.32等软件统计分析。结果福建亚种猕猴20个微卫星位点共检测到等位基因200个,观察等位基因数(Na)为 10.0000±3.1119; 有效等位基因数(Ne)为6.8438±2.4905; 期望杂合度(He)为0.8509±0.0564; 观察杂合度(Ho)为0.4607±0.1247; 香隆信息指数(I)为2.0166±0.3463; 多态信息含量(PIC)为0.8154±0.0665; 显示检测的20个微卫星DNA位点均存在高度的遗传多态性,其中有17个位点偏离Hardy-Weinberg平衡(P<0.01)。结论有效地分析了福建亚种猕猴群体的遗传多态性,为今后建立福建亚种猕猴遗传质量监测方法提供理论依据。

关键词: 微卫星标记, 福建亚种猕猴, 遗传多样性

Abstract: Objective To analyze the genetic diversity of Macaca mulatta littoralis and providing basic data for genetic quality monitoring methods of the population. Methods Twenty-eight Macaca mulatta littoralis were genotyped by using 20 microsatellite DNA markers, DNA was extracted from blood and amplified by PCR, and PCR products were analyzed by non-modified polyacrylamide gel electrophoresis. Every microsatellite locus was calculated by software POPGENE 1.32 and Excel. Results All 20 microsatellite loci were highly polymorphic, 200 alleles were found in the population.The number of alleles (Na), effective number of alleles (Ne), expected heterozygosity (He), observed heterozygosity (Ho), Shannon's information index (I) and polymorphism information content (PIC) were ranged from 5 to 16, 3.7333 to 11.6148, 0.7455 to 0.9305, 0.3214 to 0.7500, 1.3998 to 2.5963, and 0.6862 to 0.9074, with an average of 10, 6.8438, 0.8509, 0.4607, 2.0166 and 0.8154, respectively. All of 17 loci showed significant deviations from Hardy-Weinberg equilibrium. Conclusions There are high genetic diversity in Macaca mulatta littoralis of this colony. The experiments can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci.

Key words: Microsatellite marker, Macaca mulatta littoralis, Genetic diversity

中图分类号:

Q95-33

周建华, 李志雄, 杨燕燕, 谢金东, 俞春英, 王训立. 应用微卫星DNA标记对福建亚种猕猴遗传多样性的分析[J]. 实验动物与比较医学, 2017, 37(6): 434-441.

ZHOU Jian-hua, LI Zhi-xiong, YANG Yan-yan, XIE Jin-dong, YU Chun-ying, WANG Xun-li. Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers[J]. Laboratory Animal and Comparative Medicine, 2017, 37(6): 434-441.

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应用微卫星DNA标记对福建亚种猕猴遗传多样性的分析

Analysis on Genetic Diversity of Macaca mulatta littoralis by Using Microsatellite DNA Markers

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