几种炎症因子在小鼠跨区供血耳瓣choke区血管新生过程中的作用

doi:10.3969/j.issn.1674-5817.2017.06.002

实验动物与比较医学 ›› 2017, Vol. 37 ›› Issue (6): 428-433.DOI: 10.3969/j.issn.1674-5817.2017.06.002

几种炎症因子在小鼠跨区供血耳瓣choke区血管新生过程中的作用

温福利¹, 梁成¹, 庄跃宏², 马雷¹, 郑和平¹

1.福建医科大学福总临床医学院(南京军区福州总医院)比较医学科, 福州 350025;
2.福建医科大学人体解剖与组织胚胎学系, 福州 350108

收稿日期:2017-10-25 出版日期:2017-12-25 发布日期:2017-12-25
作者简介:温福利(1987-),男,技师,硕士研究生,研究方向:比较医学。E-mail:wen_fuli@163.com
基金资助:
福建省自然科学基金资助项目(2015J01412)和全军实验动物科研课题(SYDW(2016)007)

The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice

WEN Fu-li¹, LIANG Cheng¹, ZHUANG Yue-hong², Ma Lei¹, ZHENG He-ping¹

1. Department of Comparative Medicine, Fujian Medical University Affiliated Fuzhou General Hospital of Nanjing Command, PLA, Fuzhou 350025, China;
2. Department of human Anatomy and History and Embryo, Fujian Medical University, Fuzhou 350108, China

Received:2017-10-25 Online:2017-12-25 Published:2017-12-25

摘要/Abstract

摘要： 目的探讨几种炎症因子在choke区血管新生过程中的作用。方法建立小鼠跨区供血耳瓣模型,切取小鼠耳瓣,HE染色后,观察血管管径变化。运用免疫组织化学技术,分别在造模术后当日(0 d)、1 d、3 d、5 d、7 d检测细胞间黏附分子-1(ICAM-1)、单核细胞趋化蛋白-1(MCP-1)、巨噬细胞标记物(CD68)的表达情况。结果对小鼠耳瓣血管管径观察显示,术后3 d可见新生血管, 7 d后血管扩张明显。在术后3 d, 两个choke区中CD68标记的巨噬细胞聚集量最大,MCP-1和ICAM-1在术后早期炎症反应期间出现高表达。choke区血管扩张期间,组织中的三种炎症因子表达明显增多。结论 ICAM-1、CD68、MCP-1三种炎症因子在血管重塑中起重要作用,适当的炎症反应对皮瓣存活起到积极作用。

关键词: 免疫组织化学, 炎症因子, choke区, 血管

Abstract: Objective To explore the role of inflammation in angiogenesis in choke area. Methods The ear flap model of mice was established and the changes of blood vessel diameter was observed by HE staining. The expressions of intercellular cell adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein-1 (MCP-1) and cluster of differentiation 68 (CD68 ) were detected by immunohistochemistry on the day after modeling(0 d), 1 d, 3 d, 5 d and 7 d, respectively. Result By observing the vascular diameter of the ear flap in mice, it was found that the vessels were visible at 3 d after operation and the vascular dilatation was obvious at 7 d. CD68-labeled macrophages had the largest accumulation in two choke area at 3 d. High expression of MCP-1 and ICAM-1 was observed in the early stage of postoperative inflammatory response. The expression of the three inflammatory factors in the tissue was increased significantly during the vascular dilation. Conclusion The three inflammatory factors (ICAM-1, CD68 and MCP-1) play an important role in vascular remodeling. Proper inflammatory response plays a positive role in flap survival.

Key words: Immunohistochemistry, Inflammatory factor, Choke area, Vessels

中图分类号:

Q95-33

温福利, 梁成, 庄跃宏, 马雷, 郑和平. 几种炎症因子在小鼠跨区供血耳瓣choke区血管新生过程中的作用[J]. 实验动物与比较医学, 2017, 37(6): 428-433.

WEN Fu-li, LIANG Cheng, ZHUANG Yue-hong, Ma Lei, ZHENG He-ping. The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(6): 428-433.

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几种炎症因子在小鼠跨区供血耳瓣choke区血管新生过程中的作用

The Role of MCP-1, CD68 and ICAM-1 in the Process of Angiogenesis in Choke Area of Mice

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