dl -扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜刺激性的研究

doi:10.3969/j.issn.1674-5817.2015.05.003

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (5): 356-361.DOI: 10.3969/j.issn.1674-5817.2015.05.003

dl -扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜刺激性的研究

夏敏杰¹, 黄超¹, 田芳¹, 王玉柱¹, 职瑞娜^1,2, 丁训城¹, 严钱勤³, 李卫华¹

1.上海市计划生育科学研究所, 国家计划生育药具重点实验室, 上海 200032;
2.复旦大学公共卫生学院, 上海 200032;
3.上海市第六人民医院, 上海 200233

收稿日期:2015-01-27 出版日期:2015-10-25 发布日期:2015-10-25
作者简介:夏敏杰(1983-), 男, 硕士, 研究实习员, 遗传学。E-mail: mito1983@163.com。
基金资助:
国家科技重大专项(2012ZX10001007-009-003,2013ZX10001006-003-002); 十二五国家科技支撑计划(2012BAI31B04); 国家自然科学基金青年基金项目 (81100459); 上海市科委项目(13431900800); 上海市人口和计划生育委员会(2012JG03)

Study on Vaginal Irritation of dl-Mandelic Acid Thermosensitive in situ Gel in Miniature Pig

XIA Min-jie¹, HUANG Chao¹, TIAN Fang¹, WANG Yu-zhu¹, ZHI Rui-na^1,2, DING Xun-cheng¹, Yan Qian-Qin³, LI Wei-hua¹

1. State Key Labs of Family Planning Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, 200032, China;
2. School of public health, Fudan University, Shanghai 200032, China;
3.The Sixth People's Hospital, Shanghai 200233, China

Received:2015-01-27 Online:2015-10-25 Published:2015-10-25

摘要/Abstract

摘要： 目的研究dl-扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜的刺激性。方法将9只雌性小型猪按体质量随机分为空白胶组、dl-扁桃酸阴道热敏凝胶剂组和市售壬苯醇醚凝胶(N-9凝胶)组, 肌肉注射苯甲酸雌二醇400 μg/kg(促发情), 12 d后建立小型猪阴道刺激性试验模型,阴道给予5 mL凝胶, 每日一次, 连续给药7 d, 在给药前和末次给药后的24 h与72 h,收集阴道灌流(CVL)液, 流式细胞仪计数炎性细胞和中性粒细胞数目; 末次给药72 h后处死小型猪, 取阴道组织, 观察阴道黏膜组织病理学改变。结果 dl-扁桃酸阴道热敏凝胶剂组CVL液中炎性细胞、中性粒细胞数目明显低于N-9 凝胶组(P<0.05)。小型猪阴道黏膜组织HE染色显示, dl-扁桃酸阴道热敏凝胶剂处理组阴道黏膜无明显上皮破损、血管充血以及炎症细胞浸润, 其刺激性评分(5.10±0.47), 与空白胶组(3.30±1.60)比较无显著性差异(P>0.05),明显低于N-9 凝胶组(20.50±3.50)(P<0.05)。结论 dl-扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜刺激性较小。

关键词: dl-扁桃酸阴道热敏凝胶剂, 小型猪, 炎性细胞, 中性粒细胞

Abstract: Objective To study the vaginal irritation of dl-Mandelic acid thermosensitive in situ Gel(dl-Mandelic acid-TISG) in miniature pigs. Methods Nine miniature pigs injected with estradiol benzoate (400 μg/kg) were randomly divided into three groups: control group, dl-Mandelic acid -TISG group and nonoxynol-9 gel (N-9 gel) group. Each miniature pig was administrated with 5 mL gel through vagina once daily for 7 days. Cervicovaginal lavage (CVL) fluids were collected before administration and at the 24 h and 72 h after the last administration. The number of inflammatory cells and neutrophilic granulocytes in the CVL were detected by the flow cytometry. The miniature pigs were sacrificed at 72 h after the last administration. Histopathological changes of the vaginal mucosa were observed under light microscope. Results The number of inflammatory cells and neutrophilic granulocytes in the dl-Mandelic acid-TISG group were significantly lower than that in the N-9 group (P<0.05). Histological scoring of vaginal mucosa showed that there were no obvious mucosal epithelium damage, vascular congestion and inflammatory cell infiltration (5.10±0.47) in the dl-Mandelic acid-TISG group.There was no significant difference (3.30±1.60, P>0.05) compared with the control group (P>0.05) but significant difference compared with the N-9 group (20.50±3.50, P<0.05). Conclusion The vaginal irritation of dl-Mandelic acid-TISG in miniature pigs is mild.

Key words: dl-Mandelic acid-TISG, Miniature pig, Inflammatory cells, Neutrophilic granulocytes

中图分类号:

Q95-33

夏敏杰, 黄超, 田芳, 王玉柱, 职瑞娜, 丁训城, 严钱勤, 李卫华. dl -扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜刺激性的研究[J]. 实验动物与比较医学, 2015, 35(5): 356-361.

XIA Min-jie, HUANG Chao, TIAN Fang, WANG Yu-zhu, ZHI Rui-na, DING Xun-cheng, Yan Qian-Qin, LI Wei-hua. Study on Vaginal Irritation of dl-Mandelic Acid Thermosensitive in situ Gel in Miniature Pig[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 356-361.

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dl -扁桃酸阴道热敏凝胶剂对小型猪阴道黏膜刺激性的研究

Study on Vaginal Irritation of dl-Mandelic Acid Thermosensitive in situ Gel in Miniature Pig

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