保留不同长度十二指肠的DJB术后GK大鼠肠道激素的研究

doi:10.3969/j.issn.1674-5817.2014.03.011

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (3): 223-227.DOI: 10.3969/j.issn.1674-5817.2014.03.011

保留不同长度十二指肠的DJB术后GK大鼠肠道激素的研究

陈立新¹, 赵先哲², 乔伟伟², 顾坚忠¹, 王龙霞²

1.上海斯莱克实验动物有限责任公司, 上海 201615;
2.复旦大学实验动物科学部, 上海 200032

收稿日期:2013-11-07 出版日期:2014-06-25 发布日期:2014-06-25
作者简介:陈立新(1978-), 男, 兽医学硕士, 主要从事实验动物模型的研究。E-mail: chenlixin1224@163.com
基金资助:
上海市科学技术委员会科研计划项目(No.11140900702)

Study on Gut Hormones in GK Rats after Different Length Duodenum-Preserving Duodenal-Jejunal Bypass Surgery

CHEN Li-Xin¹, ZHAO Xian-zhe², QIAO Wei-wei², GU Jian-Zhong¹, WANG Long-Xia²

1. Shanghai SLAC Laboratory Animal CO. LTD, Shanghai 201615, China;
2. Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China

Received:2013-11-07 Online:2014-06-25 Published:2014-06-25

摘要/Abstract

摘要： 目的建立保留不同长度十二指肠十二指肠-空肠转流术(DJB)手术动物模型,探讨术后GK大鼠肠道激素的变化规律。方法雄性GK大鼠24只随机分为4组(n=6),I组: 假手术组; Ⅱ组: DJB手术组; Ⅲ组: 十二指肠保留8 mm DJB手术组; Ⅳ组: 十二指肠保留18 mm DJB手术组。分别于DJB术后9周进行颈动脉置管术,置管后1周行口服糖耐量试验及采血检测肠道激素GLP-1及GIP的变化情况。结果所有大鼠都能长期存活; Ⅲ组GK大鼠的糖耐量、第一时相胰岛素分泌量及GLP-1的分泌量均较I组有显著提高(P<0.05),GIP初始分泌量较I组有显著降低(P<0.05); Ⅳ组GK大鼠的糖耐量、第一时相胰岛素及GLP-1的分泌量、GIP初始分泌量较I组无明显差异(P>0.05); II组GK大鼠的第一时相胰岛素及GLP-1的分泌量显著高于IV组(P<0.05),在30 min时GIP 分泌量显著高于IV组(P<0.05)。结论胃肠转流手术控制血糖的水平一方面是通过重新建立抑胰岛素分泌激素及促胰岛素分泌激素新的分泌量平衡; 另一方面则是通过降低GIP的分泌量,使机体的胰岛素抵抗情况得以改善。

关键词: II型糖尿病, GK大鼠, 十二指肠-空肠转流术, 十二指肠保留, 胰高血糖素样肽-1, 葡萄糖依赖促胰岛素多肽

Abstract: Objective To establish the different length duodenum-preserving duodenal-jejunal bypass model in GK rats and explore the changes in gut hormones after surgery. Methods Male Goto-kakizaki diabetic rats(GK, n=24) were randomly divided into four groups: group I (sham group), group II(regular DJB surgery), group III (8mm duodenum-preserving DJB surgery), group IV(18mm duodenum-preserving DJB surgery). Carotid artery intubation was performed ninth week after surgery. OGTT was performed one week after carotid artery intubation . The change of the level of the serum index (blood glucose, insulin, GLP-1, GIP) during OGTT was measured. Results All GK rats after surgery were with a long-term survival. The OGTT, first-phase insulin and GLP-1 secretion in group III were improved significantly compared with group I (P<0.05) and the initial secretion of GIP in group III was significantly fewer than group I (P<0.05). The OGTT, first-phase insulin and GLP-1 secretion , the initial secretion of GIP in group IV had no significant difference compared with group I (P>0.05). The first-phase insulin and GLP-1 secretion in group II were significantly higher than group IV (P<0.05) and the GIP secretion at 30 min was significantly higher than group IV (P<0.05). Conclusions On the basis of experimental results, the mechanisms of DJB surgery improving blood glucose level in GK rat might be closely related to the restoration of balance between anti-incretin factors and incretin factors. On the other hand, DJB surgery can improve insulin-resistance in GK rat through reducing the secretion of GIP.

Key words: Type 2 diabetes mellitus, Goto-kakizaki rat, Duodenal-jejunal bypass surgery, Duodenum-preserving, Glucagon-like peptide-1, Glucose-dependent insulinotropic polypeptide

中图分类号:

Q95-33

陈立新, 赵先哲, 乔伟伟, 顾坚忠, 王龙霞. 保留不同长度十二指肠的DJB术后GK大鼠肠道激素的研究[J]. 实验动物与比较医学, 2014, 34(3): 223-227.

CHEN Li-Xin, ZHAO Xian-zhe, QIAO Wei-wei, GU Jian-Zhong, WANG Long-Xia. Study on Gut Hormones in GK Rats after Different Length Duodenum-Preserving Duodenal-Jejunal Bypass Surgery[J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 223-227.

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保留不同长度十二指肠的DJB术后GK大鼠肠道激素的研究

Study on Gut Hormones in GK Rats after Different Length Duodenum-Preserving Duodenal-Jejunal Bypass Surgery

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