EGCG对Goto-kakizaki大鼠骨骼肌组织GLUT4表达的影响

doi:10.3969/j.issn.1674-5817.2011.06.004

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (6): 418-421.DOI: 10.3969/j.issn.1674-5817.2011.06.004

EGCG对Goto-kakizaki大鼠骨骼肌组织GLUT4表达的影响

赵先哲¹, 张江², 乔伟伟¹, 陈立新³, 顾坚忠³

1.复旦大学实验动物科学部, 上海 200032;
2.上海农林职业技术学院; 上海 201600;
3.上海斯莱克实验动物有限责任公司, 上海 201615

收稿日期:2011-01-08 出版日期:2011-12-25 发布日期:2011-12-25
作者简介:赵先哲(1984-),男,在读硕士,主要从事实验动物模型的研究

The Effect of Epigalloca-techin-3-gallate(EGCG) on Expression of GLUT4 in Skeletal Muscle Tissue of Goto-kakizaki Rats

ZHAO Xian-zhe, ZHANG Jiang, QIAO Wei-wei, CHEN Li-xin, GU Jian-zhongzhong

Department of Laboratory Animal Science, Fudan University Shanghai 200032, China

Received:2011-01-08 Online:2011-12-25 Published:2011-12-25

摘要/Abstract

摘要： 目的研究表没食子儿茶素没食子酸酯(EGCG)对自发性II型糖尿病GK大鼠的作用及对骨骼肌组织葡萄糖转运蛋白4(GLUT4)表达的影响。方法自发性2型糖尿病大鼠GK大鼠40只,同系健康对照Wistar大鼠10只, 大鼠随机分为:正常对照组、II型糖尿病组、II型糖尿病低剂量EGCG(50 mg/kg)治疗组、中剂量(100 mg/kg)EGCG、高剂量EGCG(300 mg/kg)治疗组, 6周后测血糖、血脂和骨骼肌GLUT4的水平。结果 EGCG可明显改善GK大鼠的OGTT及血脂,增加大鼠模型骨骼肌胞膜上GLUT4蛋白表达。结论 EGCG可降低TIIDM大鼠血糖水平, 其机制与提高糖尿病大鼠骨骼肌中GLUT4表达有关。

关键词: 表没食子儿茶素没食子酸酯(RGCG), GK大鼠, 葡萄糖转运蛋白4(GLUT4)

Abstract: Objective To investigate the effect of epigalloca-techin-3-gallate(EGCG)on expression of GLUT4 in the skeletal muscle tissue of Goto-kakizaki rats. Methods Goto-kakizaki diabetic rats (GK, n=40) were observed and Wistar rat (n=10) as control. Rats were randomly divided into fIve groups: normal control group, TIIDM model group , diabetic model plus EGCG of 50mg/kg, 100mg/kg and 300mg/kg therapy group .After 6 weeks,blood glucose,serum lipids and the expression of GLUT4 were detected. Results EGCG obviously improved the OGTT and serum lipids, and increased the expression of GLUT4 in skeletal muscle membrane fraction. Conclusion EGCG can decrease the levels of blood glucose in type II diabetic rats, and its mechanisms might be closely related to the elevation of the level of GLUT4 in the skeletalmuscle tissues of rats.

Key words: Epigalloca-techin-3-gallate(EGCG), Goto-kakizaki rat, GLUT-4

中图分类号:

Q95-33

赵先哲, 张江, 乔伟伟, 陈立新, 顾坚忠. EGCG对Goto-kakizaki大鼠骨骼肌组织GLUT4表达的影响[J]. 实验动物与比较医学, 2011, 31(6): 418-421.

ZHAO Xian-zhe, ZHANG Jiang, QIAO Wei-wei, CHEN Li-xin, GU Jian-zhongzhong. The Effect of Epigalloca-techin-3-gallate(EGCG) on Expression of GLUT4 in Skeletal Muscle Tissue of Goto-kakizaki Rats[J]. Laboratory Animal and Comparative Medicine, 2011, 31(6): 418-421.

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EGCG对Goto-kakizaki大鼠骨骼肌组织GLUT4表达的影响

The Effect of Epigalloca-techin-3-gallate(EGCG) on Expression of GLUT4 in Skeletal Muscle Tissue of Goto-kakizaki Rats

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