玉米肽和有氧运动对肥胖大鼠血浆Glucagon、MCP-1、PP和PYY水平的影响

doi:10.3969/j.issn.1674-5817.2014.03.009

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (3): 214-218.DOI: 10.3969/j.issn.1674-5817.2014.03.009

玉米肽和有氧运动对肥胖大鼠血浆Glucagon、MCP-1、PP和PYY水平的影响

李翔, 史仍飞, 娄淑杰

上海体育学院运动科学学院, 上海200438

收稿日期:2014-03-07 出版日期:2014-06-25 发布日期:2014-06-25
作者简介:李翔(1988-), 男, 硕士研究生,E-mail: lxzz1988@126.com
基金资助:
上海市科委能力建设项目(NO.11290503000); 上海市人类运动能力开发与保障重点实验室(上海体育学院)(NO.11DZ2261100); 上海市地方高校大文科研究生学术新人培育计划项目(NO.xsxr2013045)

Effects of Corn Peptide and Aerobic Exercise on Levels of Glucagon、MCP-1、PP and PYY in Plasma of Obese Rats

LI Xiang, SHI Reng-fei, LOU Shu-jie

School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China

Received:2014-03-07 Online:2014-06-25 Published:2014-06-25

摘要/Abstract

摘要： 目的通过观察玉米肽和有氧运动对肥胖大鼠血浆内胰高血糖素(Glucagon)、单核细胞趋化蛋白-1(monocyte chemoattractant protein -1,MCP- 1)、胰多肽(Pancreatic polypeptide, PP)和酪酪肽(Peptide YY,PYY)水平的影响,探讨其减控大鼠体质量的可能激素相关机制。方法雄性SD大鼠150只,随机选取15只作为普通饲料组(C组),给予普通饲料,其余135只给予高脂饲料以建肥胖模型。8周后将40只雄性肥胖大鼠随机分为肥胖对照组(OC组)、酪蛋白组(Cas组)、玉米肽组(CP组)、运动组(OE组)和玉米肽运动组(OEC组), 其中OE组和OEC组进行4周的有氧运动。使用ELISA方法检测血浆中Glucagon、MCP-1、PP和PYY水平。结果建模成功后经过4周干预,OC组大鼠体质量显著高于C组,OE组和OEC组大鼠体质量与OC组相比显著降低。OC组大鼠血Glucagon水平与C组无显著差异,CP组大鼠血Glucagon水平与OC组相比显著降低。OC组大鼠血MCP-1水平显著高于C组,CP组、OE组和OEC组与OC组大鼠血MCP-1水平相比显著降低。OC组大鼠血PYY水平均显著低于C组,OEC组大鼠血PYY水平显著高于OC组和CP组; 各组大鼠间血PP水平均无显著性差异。结论单纯服用玉米肽可以降低肥胖大鼠血Glucagon和MCP-1水平。玉米肽结合有氧运动可降低肥胖大鼠体质量以及血液内的MCP-1水平,并提高肥胖大鼠血液内PYY水平。

关键词: 玉米肽, 有氧运动, 胰高血糖素, 单核细胞趋化蛋白-1(MCP-1), 胰多肽(PP), 酪酪肽(PPY)

Abstract: Objective To investigate the effects of corn peptide and aerobic exercise on the Glucagon, MCP-1(monocyte chemoattractant protein -1), PP(Pancreatic polypeptide) and PYY (Peptide YY) plasma levels for analyzing the hormonal related regulation of the body weight lose in obese rats. Methods Fifteen male SD rats were randomly selected from 150 as normal diet group (C). The rats of C were given a normal diet , while the remaining 135 were given a high fat diet. 8 weeks later the 40 male obese rats were randomly divided into obesity control group (OC) , casein supplement group (Cas), corn peptide supplement group (CP) , obese rats exercise group (OE) and corn peptide supplement combined obese rats exercise group (OEC). OE and OEC group rats took aerobic exercise during 4 weeks intervention. ELISA method was used to test the Glucagon, MCP-1, PP and PYY levels in the rats plasma. Results Four weeks intervention later, the body weight of OC was significantly higher than C, and the body weight of OE and OEC group were significantly lower than OC. There was no significant difference between OC and C in Glucagon level, but the Glucagon level of CP was significantly lower than OC. The MCP-1 level in OC group was significantly higher than C. and compared with OC, the MCP-1 levels of CP, OE and OEC decreased significantly . The PYY levels of OC were significantly lower than C, the PYY level of OEC was significantly higher than OC and CP. There was no significant difference among all groups for PP plasm level. Conclusion Corn peptides can reduce the plasma levels of Glucagon and MCP-1 in obese rats. The aerobics combined with corn peptide supplyment can reduce the plasma level of MCP-1 and increase the plasma level of PYY in rats.

Key words: Corn peptide, Aerobic exercise, Glucagon, MCP-1, PP, PYY

中图分类号:

Q95-33

李翔, 史仍飞, 娄淑杰. 玉米肽和有氧运动对肥胖大鼠血浆Glucagon、MCP-1、PP和PYY水平的影响[J]. 实验动物与比较医学, 2014, 34(3): 214-218.

LI Xiang, SHI Reng-fei, LOU Shu-jie. Effects of Corn Peptide and Aerobic Exercise on Levels of Glucagon、MCP-1、PP and PYY in Plasma of Obese Rats[J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 214-218.

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玉米肽和有氧运动对肥胖大鼠血浆Glucagon、MCP-1、PP和PYY水平的影响

Effects of Corn Peptide and Aerobic Exercise on Levels of Glucagon、MCP-1、PP and PYY in Plasma of Obese Rats

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