关键词: 巴马小型猪, 动脉粥样硬化模型, 单核细胞趋化蛋白-1(MCP-1), 核因子-κB p65(NF-κB p65), 细胞间黏附分子1(VCAM-1), 血管细胞黏附分子1(VCAM-1)

Abstract: Objective To observe the expression of MCP-1, NF-κB p65, ICAM-1and VCAM-1 protein in the abdominal aorta and coronary of the atherosclerosis (AS) model of Bama mini-pig induced by high fat diet. Method Bama mini-pigs which were 4-5 months of age are divided into normal control group which are fed basal diet for 24 weeks normal and model group which are fed high fat diet for 24 weeks, each group included 5 ones. Then the abdominal aorta and coronary of these mini-pigs were taken to conduct oil red “O” fat stain and routine HE staining. The MCP-1, NF-κB p65, ICAM-1 and VCAM-1 protein expression were observed by Immunohistochemistry staining. Result The result of oil red “O” staining shows that arty streak deposition was visibly on the abdominal aortic in model group and HE staining showed that in model group, abdominal aorta obviously thickening which is type IV of AS lesions and the formation of atherosclerotic plaque in coronary artery. The result of Immunohistochemistry staining was that, to contrast with normal group, the MCP-1, NF-κB p65, ICAM-1and VCAM-1 protein expression in abdominal aorta of model group are significantly higher(P<0.05); the MCP-1, NF-κB p65, ICAM-1and VCAM-1 protein expression in coronary of model group are significantly higher(P<0.05) too. Conclusion Abdominal aorta and coronary artery lesions of the AS model of Bama miniature pig consistent with clinical pathological characteristics of human AS. The MCP-1, NF-κB p65, ICAM-1and VCAM-1 protein expression are rise in different degree, which consistent with the clinical AS inflammatory reaction and mechanism.

Key words: Bama mini-pig, Atherosclerosis (AS) model, MCP-1, NF-κB p65, ICAM-1, VCAM-1