实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (4): 271-274.DOI: 10.3969/j.issn.1674-5817.2013.04.005

所属专题: 实验动物资源开发与利用

• 论著 • 上一篇    下一篇

巴马小型猪不同发情周期的阴道细胞学观察

田芳1, 夏敏杰1, 王玉柱1, 周新初2, 李卫华1, 丁训诚1   

  1. 1.上海市计划生育科学研究所, 上海 200032;
    2.上海交通大学农业与生物学院, 上海 201101
  • 收稿日期:2012-11-01 出版日期:2013-08-25 发布日期:2013-08-25
  • 作者简介:田芳,女,硕士,研究实习员,研究方向:生殖毒理学,E-mail:iamtianfang@gmail.com
  • 基金资助:
    国家科技重大专项(2012ZX10001-007-009); 十二 五国家科技支撑项目(2012BAI31B04); 国家自然 科学基金青年基金项目(81100459); 上海市科委项 目(11PJ1408400,11140902900)

Vaginal Cytologic Examination on Estrous Cycle in Mini-pig

TIAN Fang1, XIA Min-jie1, WANG Yu-zhu1, ZHOU Xin-chu2, LI Wei-hua1, DING Xun-cheng1   

  1. 1. Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China;
    2. Shanghai Jiao Tong University School of Agriculture and Biology, Shanghai 201101, China
  • Received:2012-11-01 Online:2013-08-25 Published:2013-08-25

摘要: 目的 研究广西巴马小型猪不同发情周期的阴道细胞学特点。方法 选取发情间期、发情前期和发情期小型猪各4只,阴道涂片,分别进行HE染色和巴氏染色。光学显微镜下观察阴道涂片细胞形态, 进行分类和计数。结果 广西巴马小型猪阴道涂片中HE染色和巴氏染色均可见白细胞、副基细胞、小中间细胞、大中间细胞和角化上皮细胞,发情间期: 白细胞百分比明显高于发情前期和发情期(P<0.01); 发情前期: 副基细胞和小中间细胞明显高于发情间期和发情期(P<0.01); 发情期: 大中间细胞和角化上皮细胞明显高于发情间期和发情前期(P<0.01)。结论 阴道涂片法可作为确定广西巴马小型猪发情周期的辅助方法,对小型猪的繁殖育种有一定的应用价值。

关键词: 阴道涂片, 发情周期, 小型猪

Abstract: Objective To examine the vaginal cytology of estrous cycle in Guangxi Bama mini-pig. Methods Twelve mini-pig were selected and divided into different groups-diestrus group, proestrus group and estrus group. Vaginal smears of the pigs were obtained and stained with hematologic stain and Papanicolaou stain respectively .Epithelial cell morphology was observed and counted under the optical microscope. Results Leukocytes, para basal cell, small intermediate cell, large intermediate cell and superficial cell were found in vaginal smears. Leukocyte percentage was significantly higher during diestrus than that during estrus or proestrus (P<0.01). Para basal cell plus small intermediate cell counts were significantly higher during proestrus than that during estrus or diestrus (P<0.01). Superficial plus large intermediate cell counts were significantly higher during estrus than that during diestrus or proestrus (P<0.01). Conclusion Vaginal smears can be used to estimate different stage of estrous cycle in Guangxi Bama mini-pig.

Key words: Vaginal smear, Estrous cycle, Mini-pig

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