慢性心肌缺血小型猪的氧化应激反应指标观测

doi:10.3969/j.issn.1674-5817.2012.05.006

摘要/Abstract

摘要： 目的观测慢性心肌缺血小型猪的氧化应激反应指标。方法 15只巴马小型猪随机分成3组,即正常对照(Ctr)组,高脂(HF)组和慢性心肌缺血(CMI)组,每组5只。采用维生素D₃和异丙肾上腺素复合高脂饮食建立慢性心肌缺血模型,连续24周。造模24周后,观察各组血清CK-MB、血小板聚集率及心肌组织中SOD、MDA、GSH-Px、NO、LD和ATP酶含量的变化。结果与Ctr组比, CMI组血清CK-MB活性、PAR、心肌组织中MDA和LD含量均显著升高(P<0.05, P<0.01), 心肌组织中SOD、SOD/MDA比值、GSH-Px、NO、Na⁺-K⁺-ATP、Ca⁺⁺-ATP和Mg⁺⁺-ATP酶活性均明显降低(P<0.05, P<0.01), 而HF组PAR和心肌组织中SOD/MDA比值、MDA含量均升高显著(P<0.05, P<0.01), 心肌组织中SOD、GSH-Px、Na⁺-K⁺-ATP、Mg⁺⁺-ATP酶活性均降低显著(P<0.05); 与HF组比,CMI组血清CK-MB活性明显高于HF组(P<0.01),心肌组织中Na⁺-K⁺-ATP酶和Ca⁺⁺-ATP活性显著低于HF组(P<0.05, P<0.01); 相关分析显示,CMI组CK-MB活性与PAR和MDA 含量呈显著正相关(P<0.05, P<0.01), 与SOD、SOD/MDA、GSH-Px、NO呈显著负相关(P<0.05, P<0.01); 血小板聚集率与CK-MB和MDA呈显著正相关(P<0.05),与SOD、SOD/MDA和Na⁺-K⁺-ATP酶活性呈显著负相关(P<0.05, P<0.01); 心肌组织中LD含量与MDA呈显著正相关(P<0.05), 与SOD/MDA、Na⁺-K⁺-ATP、Ca⁺⁺-ATP和Mg⁺⁺-ATP呈显著负相关(P<0.05); 心肌组织中NO水平与SOD、SOD/MDA、GSH-Px呈显著正相关(P<0.05, P<0.01),与CK-MB和MDA呈显著负相关(P<0.05)。结论氧化应激反应参与了慢性心肌缺血的形成过程。

关键词: 氧化应激, 慢性心肌缺血, 小型猪

Abstract: Objective To observe the effects of oxidative stress on chronic myocardial ischemia in mini-pigs. Methods Fifty-five Bama mini-pigs were randomly divided into three groups: normal control (Ctr) group, high-fat (HF) group and chronic myocardial ischemia (CMI) group, five animals in each group. The chronic myocardial ischemia model was made induced by vitamin D₃, isoproterenol and high-fat diet for 24 weeks. The changes of phosphokinase isoenzyme (CK-MB), platelet aggregation rate (PAR) and the SOD, MDA, GSH-Px, NO, LD, ATPase in myocardial tissues were detected. Results Compared with normal control group, the serum CK-MB activity, PAR, the myocardial tissues of MDA and LD contents in CMI group were significant increased (P<0.05, P<0.01), the myocardial tissues of SOD, SOD/MDA ratio, GSH-Px, NO, Na⁺-K⁺-ATPase, Ca⁺⁺-ATPase and Mg⁺⁺-ATPase in CMI group were lowered (P<0.05, P<0.01). PAR and the myocardial tissues of SOD/MDA ratio and MDA content in HF group were significant increased (P<0.05, P<0.01), while the myocardial tissues of SOD, GSH-Px, Na⁺-K⁺-ATPase and Mg⁺⁺-ATPase were significant decreased (P<0.05). Compared with HF group, the serum CK-MB activity in CMI group was significant higher than that of HF group (P<0.01) and the myocardial tissues of Na⁺-K⁺-ATPase and Ca⁺⁺-ATPase were significant lower than those of HF group (P<0.05, P<0.01). Correlation analysis showed that there was a positive correlation between CK-MB and PAR, MDA in CMI group (P<0.05, P<0.01), and a significant negative correlation between CK-MB and SOD, SOD/MDA, GSH-Px, NO respectively(P<0.05, P<0.01). The platelet aggregation rate was positively correlated with CK-MB and MDA (P<0.05), and was negative relation with SOD, SOD/MDA and Na⁺-K⁺-ATPase (P<0.05, P<0.01). There was a significant positively correlation between LD and MDA in myocardial tissues (P<0.05), and LD was negatively correlated with SOD/MDA, Na⁺-K⁺-ATPase, Ca⁺⁺-ATPase and Mg⁺⁺-ATPase (P<0.05). NO level was positively correlated with SOD, SOD/MDA, GSH-Px in myocardial tissues(P<0.05, P<0.01), and was negatively related with CK-MB and MDA (P<0.05). Conclusion Oxidative stress injury was participated in the formation of chronic myocardial ischemia.

Key words: Oxidative stress, Chronic myocardial ischemia, Mini-pig

中图分类号:

Q95-33

徐孝平, 潘永明, 陈亮, 徐剑钦, 王德军, 朱科燕, 杨玉伟, 陈民利. 慢性心肌缺血小型猪的氧化应激反应指标观测[J]. 实验动物与比较医学, 2012, 32(5): 401-406.

XU Xiao-ping, PAN Yong-ming, CHEN Liang, XU Jian-qin, WANG De-jun, ZHU Ke-yan, YANG Yu-wei, CHEN Min-li. Observation on Oxidative Stress in Chronic Myocardial Ischemia in Mini-pig[J]. Laboratory Animal and Comparative Medicine, 2012, 32(5): 401-406.

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