关键词: 新方法学, 3R原则, 类器官, 器官芯片, 3D生物打印, 计算模型, 实验动物伦理

Abstract:

Laboratory animals are increasingly utilized as invivo models for life sciences, medical research, and drug development, laboratory animals, and the associated ethical and welfare issues have attracted increasing attention. The "3R" principles (Replacement, Reduction, Refinement) are globally recognized guideline for conducting animal experiments, providing direction for balancing scientific research with animal welfare. New approach methodologies refer to technologies that mimic human physiological systems and can replace or reduce traditional animal experiments,, mainly including in vitro (e.g. organoids, organ-on-a-chip), in chemico (e.g. 3D bioprinting models) and in silico tools. This assay systematically reviews the technical characteristics, development history, and application of new approach methodologies in disease modeling, drug development, toxicological evaluation, etc., and focuses on the implementation path of the "3R" principle. New approach methodologies can replace some animal experiments through in vitro biomimetic models, reduce the number of experimental animals used through high-throughput screening and computational simulation, optimize experimental design and reduce animal pain through accurate dose prediction and early biomarker monitoring. New approach methodologies still face challenges such as the inability to fully recapitulate the complex human physiological system, insufficient standardization and reproducibility, and an underdeveloped validation and regulatory acceptance pathway. Nevertheless, continuous technological innovation, rigorous scientific validation, proactive international cooperation and sound policy support are expected to gradually reduce or replace traditional animal experiments and promote biomedical research in the direction of "scientific rigor, animal welfare, and cost-effectiveness".

Key words: New approach methodologies, "3R" principle, Organoids, Organ-on-a-chip, 3D bioprinting models, In silico tools, Laboratory animal ethics