Estabilishment of Cardiotoxicity Model in Zebrafish Induced by Doxorubicin

doi:10.3969/j.issn.1674-5817.2015.05.004

Abstract

Abstract: Objective To establish zebrafish model of cardiotoxicity induced by doxorubicin (DOX). Methods The wild type zebrafish was divided into 6 groups: 4 different concentrations of DOX solution, control group and 0.9% NaCl groups. To assay the expression of cardiac troponin T(cTnT) and myoglobin (Mb) by Western blot at 72 hpf (72 hours post fertilization). Morphologic changes in the cardiovascular system were observed under stereomicroscope. The expression of miRNA146a in zebrafish was tested by RT-PCR. Results DOX had no cardiotoxicity in zebrafish within 0~MNLC/10; when the concentration of DOX ≥MNLC/3, it can be induced cardiovascular toxicty in the zebrafish, manifested as blood flow slows down, the lack of blood flow, venous sinus congestion, venous sinus edema and it is dose-dependent. Tissue expression of miRNA146a on zebadfishes of 3 concentrations were all increased compared with control group (P<0.01), and it is dose-dependent. There was no significant differences in the expression of cTnT and Mb between DOX groups and control group (P>0.05). Conclusion The cardiotoxicity induced by DOX was significant and ithad a similar toxic effect to mammal. So the zebrafish model of cardiotoxicity induced by DOX was successfully established.

Key words: Doxorubicin (DOX), Cardiotoxicity, Zebrafish, Model

CLC Number:

Q95-33

ZHANG Cheng-da, ZHANG Li-li, ZHANG Li-jiang, CHEN Yun-xiang. Estabilishment of Cardiotoxicity Model in Zebrafish Induced by Doxorubicin[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 362-366.

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