Studies on Construction and Transfer of Cloned Minipig Embryos

doi:10.3969/j.issn.1674-5817.2011.05.006

Abstract

Abstract: Objective To establish the somatic cell nuclear transfer technology of minipig and provide technical support for studies of transgenic animal models. Methods Using somatic cell nuclear transfer techniques, nuclear transfer was finished with fetal fibroblast cells and ear fibroblast cells (donor cells) from minipig and Fengjing pig respectively. Embryo transfer was carried out using the two kinds of reconstructed embryos. Result There were no significant differences between the two kinds of donor cells in vitro development (P>0.05). Chemical activation after electric fusion could partially improve the blastocyst rates, but the difference was not significant (P>0.05). Four healthy cloned piglets were borned after embryos transfer by the mixture of the two kinds reconstructed embryos. Microsatellite identification of cloned piglets showed whole same with their donor cells in heredity, and had nothing to do with their surrogate sows. Conclusion The method of our study group could be used to produce clone pigs.

Key words: Nuclear transfer, Clone, Minipig, Chemical activation

CLC Number:

Q95-33

DAI Jian-jun, LI Yao, GU Xiao-long, ZHANG Ting-yu, WU Cai-feng, ZHANG Shu-shan, WU Bin, CHEN Hui-lan, CHEN Xue-jin, ZHANG De-fu. Studies on Construction and Transfer of Cloned Minipig Embryos[J]. Laboratory Animal and Comparative Medicine, 2011, 31(5): 333-337.

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