Application of Vascular-access-port in Intravenous Glucose Tolerance Test in Minipig

doi:10.3969/j.issn.1674-5817.2015.02.006

Abstract

Abstract: Objective To improve the reliability of intravenous glucose tolerance test (IVGTT) in minipigs and reduce the stress response during the test with vascular-access-port (VAP), and provides a new method which was fast and convenient and stable for IVGTT in minipigs. Methods The 12 Bama minipigs were randomly divided into the implantable VAP group and the normal control group, the difference of the two groups animals in IVGTT were compared, and the stress index were evaluated. Results VAP group and the control group could appear glucose tolerance test reactions in IVGTT, changes and the trend in serum insulin levels were not significantly different, but the glucose levels in VAP group at 0 min, 60 min, 90 min and 120 min were significantly lower than those of control group (P< 0.05, P<0.01), and significantly higher than that of the control group in 5 min (P<0.01); During the test, the CK levels in the control group were significantly higher than the VAP group at each time point (P< 0.05, P<0.01); ACTH and CORT levels in 0 min ,15-120 min in control group were higher than VAP group, and in 60-120 min had a significant difference (P<0.05, P<0.01) Conclusion Compare with conventional methods for IVGTT, VAP implantation way to do IVGTT suffered less stress in minipigs, more in line with animal welfare, and the test results were more reliable and stable.

Key words: Vascular-access-port(VAP), Minipig, Intravenous glucose tolerance test (IVGTT), Stress

CLC Number:

Q95-33

CHEN Liang, PAN Yong-ming, CAI Yue-qin, ZHU Ke-yan, XU Xiao-ping, CHEN Min-li. Application of Vascular-access-port in Intravenous Glucose Tolerance Test in Minipig[J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 114-119.

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