Establishment of Chronic Pulmonary Hypertension Model in Tibet Minipig

doi:10.3969/j.issn.1674-5817.2015.06.001

Abstract

Abstract: Objectives To establish chronic pulmonary hypertension (PH) model with monocrotaline (MCT) in young Tibet minipigs. Methods Twelve Tibet minipigs (weighing 5.0~6.0 kg, aged 2.0 months) were randomly divided into two groups and treated with MCT (12.0 mg/kg) or placebo (75% ethanol). After six weeks, the pulmonary arterial pressures of the minipigs were measured by performing right heart catheterization (RHC). The pathologic changes of small pulmonary arteries were observed. Index of right ventricular hypertrophy was also compared between two groups. Results The mean pulmonary arterial pressure by RHC was obviously increased in the MCT group than that in control group (24.62±1.38 mmHg vs 15.10±0.76 mmHg, P<0.01). The pathologic observations showed that the pulmonary vascular remodeling and the lumen disappeared in small pulmonary arteries. There was also progressive intimal fibrosis accompany with hyalinization, the lumen was occlusive, twisty and distorted with plexiform lesions. Indexes of right ventricular hypertrophy of MCT group were significantly higher (0.36±0.16 vs 0.28±0.13, P<0.05). Conclusions An animal model of chronic PH was established successfully in young minipigs at six weeks after MCT was intraperitoneally injected. The features of the chronic PH model of minipigs are similar with the primary PH parameters.

Key words: Pulmonary hypertension, Monocrotaline(MCT), Tibet minipig, Right heart catheterization

CLC Number:

Q95-33

LIU Rong, HOU Meng, LIAO Hai-xing, ZHANG Xin-feng, ZHAO Jin, LIU Bao-hua, QIAN Yuan-xin, WU Qing-hong, MO Zi-yao, LIAO Dong-jiang, LI Hong-tao, GU Wei-wang. Establishment of Chronic Pulmonary Hypertension Model in Tibet Minipig[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 431-435.

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