Abstract: Objective To establish a peritoneal adhesion model in Wuzhishan minipigs. Methods Fourteen Wuzhishan minipigs were anesthetized by Sumianxin II with intramuscular injection and pentobarbital sodium with intravenous injection after fasting overnight. Then, the abdominal cavity were opened under laparotomy. Afterwards, the peritoneal adhesion model was established by physical damage of serosal, chemical damage, vascular ligation and ligation of parietal peritoneum. Result Peritoneal adhesions were found 1 day after the operation with the degree of adhesion score 2 and adhesion area score 2~3. Peritoneal adhesions were serious 3 days after the operation with the degree of adhesion and adhesion area score 3~4. The adhesions was sustained for a long time, and peritoneal adhesions still existed 12 months after the operation with the degree of adhesion and adhesion area score 2~3. There were no obvious difference in the degree of adhesion and adhesion area between males and females. Conclusion The peritoneal adhesion model were succeesfully established in Wuzhishan minipigs by multifactors.

Key words: Wuzhishan minipigs, Peritoneal adhesion model, Multiple factor