Abstract: Objective To clone resistin gene of Macaca mulatta, its sequence has been identified and analyzed. Methods Total RNA was extracted from abdominal adipose tissue, and resistin gene was amplified by RT-PCR, cloned and inserted into vector. Sequence was identified by PCR and restriction enzyme digestion, and analyzed by DNAMAN software. The coding region was determined and compared by ORFfinder and BLAST software. Result Resist in gene (363 bp) was obtained by RT-PCR amplification and the recombinant cloning vector were positive identification by PCR and XbaI and PstI restriction enzyme digestion. Sequence analysis showed that the resistin gene homology was 96%, 67% and 65.7% with human, rat and mouse. Phylogenetic tree results showed that resistin gene of Macaca malutta has a close genetic relationship with human. The analysis results shows the resistin was coded to 108 amino acids and the homology was 92% with human. Conclusion The resistin gene of Macaca malutta was successfully cloned for laying a preliminary basis and providing experimental data in the related studies of resistin gene of Macaca malutta.

Key words: Resistin gene, Gene clone, Macaca malutta