The Application of Trichostatin A for Somatic Cell Nuclear Transfer

doi:10.3969/j.issn.1674-5817.2016.02.017

Abstract

Abstract: Somatic cell nuclear transfer (SCNT) technique has been successfully applied to obtain many mammal clonal offspring. But the success rate was always very low and most surviving individuals showed phenotypic abnormalities. Research shows that the incomplete reprogramming of reconstructed embryo is one of the main reasons that causes the low survival rate and offspring dysplasia. DNA methylation and histone acetylation are both the essential factors that influence the reprogramming. Trichostatin A (TSA ) is a streptomycin metabolite, as well the histone acetylation enzyme inhibitor that reduces the level of DNA methylation and promotes the reprogramming rate to improve the cloning efficiency. The article studies the biological function of TSA and its application in animal SCNT.

Key words: Trichostatin A (TSA ), Somatic cell nuclear transfer (SCNT), Histone acetylation

CLC Number:

Q95-33

SHI Chao, CHEN Guo-qiang, MA Yu-fang. The Application of Trichostatin A for Somatic Cell Nuclear Transfer[J]. Laboratory Animal and Comparative Medicine, 2016, 36(2): 157-160.

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