Establishment of Vaginal Mucosal Immuno-inflammatory Response Model in Minipig

doi:10.3969/j.issn.1674-5817.2016.02.001

Abstract

Abstract: Objective To establish the vaginal mucosal immuno-inflammatory model response of minipig. Methods Six female minipigs were randomly divided into the control and Nonoxynol-9 (N-9) model group (40 mg/mL). After 12 days of estradiol benzoate intramuscular injection, the test drugs (N-9) were administered through vagina to minipigs at 5 mL/d for 7 consecutive days. On the 7th day, histopathological examination of vaginal mucosa was undertaken and the pathological scores were calculated based on histological indexes including epithelial ulceration/erosion, necrosis, cell infiltration, hemorrhage, congestion and edema. Cervicovaginal lavage (CVL) was collected at 24 h and 72 h after drug administration. The percentage of neutrophilic granulocytes and CD45⁺ cells in the CVL deposited cells were detected by flow cytometry. The expression and location of CD45⁺ cells in the vaginal mucosa were assessed by using immuno-histochemical (IHC) assay. Results Histopathological examination showed obvious lesions including inflammation, bleeding and edema in the vaginal epithelium of the N-9 group. Histological score of the N-9 group (18.0±3.2) was significantly higher than that of the control (3.3±0.2) (P<0.01). The percentage of neutrophilic granulocytes in the CVL cells of N-9 group was (41.70±1.63)% (24 h) and (51.17±3.81)% (72 h), and the percentage of CD45⁺ cells in the CVL cells of N-9 group was (58.5±4.73) % (24 h) and (88.0±3.57)% (72 h), both showed significant increase compared to that of the control (P<0.01). CD45⁺ cell expression increased significantly in the N-9 group. The IOD value of N-9 group was 33.95±6.69, which was significantly higher than that of the control group (28.42±8.96) (P<0.05). Conclusion The vaginal mucosal immuno-inflammatory response model of mini-pigs could be induced by giving 40 mg/mL N-9 gel to the mini-pigs through vagina for 7 consecutive days.

Key words: Minipig, Nonoxynol-9 (N-9), Histopathology, Cervicovaginal lavage(CVL), Immuno-inflammation response, Leuckocyte common antigen-CD45

CLC Number:

Q95-33

HUANG Chao, GONG Yi-juan, TIAN Fang, WANG Yu-zhu, SUN Bing, ZHI Rui-na, IA Min-jie, DING Xun-cheng, ZHOU Xin-chu, LI Wei-hua. Establishment of Vaginal Mucosal Immuno-inflammatory Response Model in Minipig[J]. Laboratory Animal and Comparative Medicine, 2016, 36(2): 81-86.

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