Dynamic Behavioral Assessment of Vascular Dementia Rat Models Made by Bilateral Common Carotid Artery Occlusion

doi:10.3969/j.issn.1674-5817.2020.01.004

Abstract

Abstract: Objective To observe the mortality of vascular dementia (VaD) rat models induced by bilateral common carotid artery occlusion (BCCAO), and to dynamically observe the cognitive function of rats after BCCAO via Morris water maze (MWM) test. Methods Sixty-eight male SD rats with no significant difference in intelligence were randomly divided into sham operation group (9 rats) and BCCAO group (59 rats). The mortality of the two groups was recorded within 30 days after operation. MWM behavioral tests were performed in 30-34, 60-64 and 90-94 days after operation to evaluate the cognitive function of the rats in BCCAO group and to detect the rate of VaD model forming in BCCAO group. Results Within 30 days after BCCAO, the mortality rate of rats was 20.3%. In MWM behavioral test, the average speed of rats in BCCAO group was basically same as that in sham operation group, with no significant difference (P>0.05); the escaping latency of rats in BCCAO group was significantly longer than that in sham operation group at 60-64 and 90-94 days after operation (P<0.05), and the VaD model rate was 97.9% and 95.7% respectively. Conclusions BCCAO is safe and reliable method to make VaD rat models. There is no obvious motor dysfunction in the rats after operation. VaD in the model rats can be basically formed between 35 and 60 days after operation and lasted till 90 days after operation.

Key words: Vascular dementia (VaD), Animal model, Cognitive impairment, Bilateral common carotid artery occlusion (BCCAO), Test time

CLC Number:

Q95-33

CONG Lin, ZHANG Zhilong. Dynamic Behavioral Assessment of Vascular Dementia Rat Models Made by Bilateral Common Carotid Artery Occlusion[J]. Laboratory Animal and Comparative Medicine, 2020, 40(1): 22-27.

References

[1] 任庆国, 梁兵, 孟祥水. 血管性认知障碍临床诊断标准概述[J]. 实用医学杂志, 2019, 35(1):1-3.
[2] 中国痴呆与认知障碍指南写作组, 中国医师协会神经内科医师分会认知障碍疾病专业委员会. 2018中国痴呆与认知障碍诊治指南(一): 痴呆及其分类诊断标准[J].中华医学杂志, 2018, 98(13): 965-970.
[3] Zhang ZY, Liu Z, Deng HH, et al.Effects of acupuncture on vascular dementia (VD) animal models: a systematic review and meta-analysis[J]. BMC Complement Altern Med, 2018, 18(1):302-311.
[4] 黄文革, 郭芬芬, 刘慰华, 等. 血管性痴呆动物模型制作方法的改良[J]. 中国比较医学杂志, 2011, 21(5):49-52,84.
[5] 吴章福, 高晓平, 李光武. 改良双侧颈总动脉结扎法与传统法制备的大鼠慢性脑缺血模型比较[J].中国康复医学杂志, 2012, 27(3):206-210.
[6] 谭洁, 韩国栋, 张泓, 等. 改良大鼠双侧颈总动脉结扎方式建立血管性痴呆模型的评价研究[J]. 中国康复医学杂志, 2017, 32(3):264-268.
[7] 赵宪林, 方秀斌, 李东培. 大鼠血管性痴呆模型制作[J]. 中国医科大学学报, 2002, 31(3):166-167,176.
[8] 张君, 程笑, 杨欢, 等. 山奈酚对慢性脑缺血大鼠学习记忆能力的影响及机制探讨[J]. 中国药理学通报, 2017, 33(1):39-44.
[9] 张新萍. Humanin (HN)衍生物-Rattin对血管性痴呆大鼠认知功能损害的影响[D]. 济南: 山东大学, 2013:20-21.
[10] 贺晓丽, 毕明刚, 杜冠华. 慢性脑缺血大鼠脑组织线粒体蛋白质组与能量代谢相关性研究[J]. 中国药理学通报, 2012, 28(9):1200-1205.
[11] Venkat P, Chopp M, Chen J.Models and mechanisms of vascular dementia[J]. Exp Neurol, 2015, 272:97-108.
[12] Juma WM, Lira A, Marzuk A, et al.C-reactive protein expression in a rodent model of chronic cerebral hypoperfusion[J]. Brain Res, 2011, 1414:85-93.
[13] Farkas E, Luiten PG, Bari F.Permanent, bilateral common carotid artery occlusion in the rat: a model for chronic cerebral hypoperfusion-related neurodegenerative diseases[J]. Brain Res Rev, 2007, 54(1):162-180.
[14] 张海燕. 五脏温阳化瘀汤对动脉粥样硬化血管性痴呆大鼠作用机理的研究[D]. 长沙: 湖南中医药大学, 2013:41.
[15] 王丽晔, 陈志刚, 罗玉敏. 动物慢性脑缺血模型的研究现状[J]. 中国比较医学杂志, 2014, 24(4):67-73.
[16] 王淑雅. 血红蛋白载氧体在大鼠视网膜慢性低灌注损伤中对视网膜的保护作用[D]. 天津: 天津医科大学, 2012:10.
[17] 谢国丽, 傅强, 赵露, 等. 大鼠眼缺血综合征动物模型的制备[J]. 临床和实验医学杂志, 2015, 14(22):1841-1843.
[18] Kim SK, Cho KO, Kim SY.White matter damage and hippocampal neurodegeneration induced by permanent bilateral occlusion of common carotid artery in the rat: comparison between Wistar and Sprague-Dawley strain[J]. Korean J Physiol Pharmacol, 2008, 12(3):89-94.
[19] 唐慧, 张文丽, 朱莹, 等. 雌激素通过上调VEGF表达降低大鼠脑慢性低灌注诱导的微血管损伤[J]. 南方医科大学学报, 2015, 35(11):1552-1556.
[20] 陈金. 肉苁蓉总苷对血管性痴呆大鼠学习记忆、p-tau蛋白表达及蛋白组学的实验研究[D]. 济南: 山东大学,2015:22.
[21] Morris R.Developments of a water-maze procedure for studying spatial learning in the rat[J]. J Neurosci Methods,1984, 11(1): 47-60.
[22] 刘汇波, 叶翠飞, 李斌, 等. 双侧颈总动脉结扎对大鼠学习记忆功能和海马组织形态学的影响[J]. 基础医学与临床, 1998, 18(4):54-58.
[23] 李斌, 刘汇波, 郭德玉, 等. 双侧颈总动脉结扎大鼠行为学观察[J].中国实验动物学杂志, 2000, 10(1):15-18.
[24] 孙莉, 金涛, 丁艳华, 等. 慢性前脑缺血致痴呆大鼠脑血流量及行为学对比研究[J]. 中国老年学杂志, 2009, 29(8):920-922.
[25] 曹岩菁. 在血管性痴呆发生过程中tau蛋白影响囊泡转运体亚型的相关性分析[D]. 济南: 山东大学, 2016:18-20.