Effects of Icariin on Human BALB/c-nu Prostate Cancer Model in Nude Mice

doi:10.3969/j.issn.1674-5817.2018.06.005

Abstract

Abstract: Objective To explore the possible mechanism of icariin inhibiting phosphoinositide 3-kinase/protein kinase B (P13K/Akt) signaling pathway and phosphorylation of related proteins in human prostate cancer (LNCaP) BALB/c-nu nude mice. Methods Totally 40 male BALB/c-nu nude mice were randomly divided into 4 groups (control group, prostate cancer group, positive drug group and experimental group) and the human prostate cancer model was established by intraprostate injection of LNCaP. The experimental group was injected 0.8% icariin by 0.1 mL/(10 g·d) tail vein, the positive group was injected 0.1 mL/10 g tail vein P13K/Akt inhibitor LY294002, and control group and the prostate cancer group were injected the same volume of normal saline. The body weight, wet weight, volume and P13K/Akt signaling pathway-related protein expression in nude mice were compared after 4 weeks of injection. Results The wet weight, volume, P13K, P-Akt, P-AR, AR-V7 and Calcitonin of prostate cancer tissues in the experimental group were lower than those in the model group (P<0.05), while E-cadherin was higher (P<0.05). Conclusion Icariin inhibits the progress of LNCaP by regulating PI3K/ Akt signaling pathway, which is related to the phosphorylation of Akt and AR.

Key words: Icariin, BALB/c-nu nude mice, Human prostate cancer/model, Phosphoinositide 3-kinase/ protein kinase B (P13K/Akt) signaling pathway, Phosphorylation

CLC Number:

Q95-33

SONG Deng-peng, RAO Hong, HAN An-yan, WU Fu-yun, CHEN De-sen. Effects of Icariin on Human BALB/c-nu Prostate Cancer Model in Nude Mice[J]. Laboratory Animal and Comparative Medicine, 2018, 38(6): 428-433.

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