Analysis of the Birthing Behaviour of Cynomolgus Macaques

doi:10.12300/j.issn.1674-5817.2023.086

Abstract

Abstract:

Objective Observing and analyzing the delivery behaviour of cynomolgus macaques, to establish the criteria for determining the occurrence of delivery in cynomolgus macaques, and then combining with veterinary assistance in order to improve the live birth rate of cynomolgus macaques. Methods By backtracking and analyzing the surveillance videos of 112 perinatal cynomolgus macaques with a gestation period of 140 d or more from 2017 to 2021, we observed and recorded the main behavioural manifestations of the cynomolgus macaques during labour, including Valsalva's maneuver, touching and licking the birth canal, lying on their backs or stomachs, and rolling and tumbling of the body. On this basis, we established the weights of delivery-related behavioural indicators and exhaustively analysed the perinatal behavioural performances of 30 cynomolgus macaques for delivery determination. Results The perinatal behavioural validation results of the 30 cynomolgus macaques showed that the behavioural indicators of Valsalva's maneuver, touching and licking the birth canal, lying on the stomach or on the floor, body rolling and tumbling occurred with different frequencies, among which Valsalva's maneuver and lying on the stomach or on the back were the most important, with weight values of 35.5% and 27.2%, respectively. These two behaviours can be used to accurately determine the onset of parturition in cynomolgus macaques. The average live birth rate of the monkeys that were accurately determined to have given birth and were assisted by veterinarians reached 87.1%, which was significantly higher than that of the monkeys that had unassisted spontaneous deliveries, which was 63.5%, and there was a significant difference between these two rates (P<0.05). Conclusion The combination of accurate birth determination and veterinary assisted delivery can significantly increase the live birth rate of experimental cynomolgus macaques.

Key words: Cynomolgus macaques, Delivery behaviour, Video monitoring, Live birth rate

CLC Number:

Q95-33

Xinyan BIAN, Yong LU, Yan WANG, Qiang SUN. Analysis of the Birthing Behaviour of Cynomolgus Macaques[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 355-362.

Figures/Tables 6

References 19

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品名 Description	规格 Specification	数量 Quantity
芯片Central processing unit	英特尔I7-10700	1
主板Mainboard	华硕Z190-H	1
内存Random access memory	金士顿16G-2666	2
散热器Heat sink	九州风神400静音风扇	1
硬盘1 Hard disk 1	英特尔512G-SSD	1
硬盘2 Hard disk 2	希捷8TB企业级	1
机箱Crate	九州风神DS-7716N-14/16P	1
电源Power supply	华硕ROG-1200W	1
显卡Video card	华硕ROG RTX2080TI-11G	1
显示器Computer monitor	AOC AG322FCX	1

品名 Description	规格 Specification	数量 Quantity
芯片Central processing unit	英特尔I7-10700	1
主板Mainboard	华硕Z190-H	1
内存Random access memory	金士顿16G-2666	2
散热器Heat sink	九州风神400静音风扇	1
硬盘1 Hard disk 1	英特尔512G-SSD	1
硬盘2 Hard disk 2	希捷8TB企业级	1
机箱Crate	九州风神DS-7716N-14/16P	1
电源Power supply	华硕ROG-1200W	1
显卡Video card	华硕ROG RTX2080TI-11G	1
显示器Computer monitor	AOC AG322FCX	1

分娩行为/项 Delivery behaviour/item	特征向量 Eigenvector (math.)	权重值/% Weight value/%	最大特征值 Maximum eigenvalue	CI值 CI value	RI值 RI value	CR值 CR value	一次性检验结果 Test results
努责Valsalva's maneuver	1.775	35.5	5	0	1.12	0	通过
摸舔产道Touching and licking the birth canal	1.242	24.8
趴卧/躺卧Lying down	1.358	27.2
身体辗转Body rolls	0.525	10.5
翻滚Tumbling	0.100	2.0

分娩行为/项 Delivery behaviour/item	特征向量 Eigenvector (math.)	权重值/% Weight value/%	最大特征值 Maximum eigenvalue	CI值 CI value	RI值 RI value	CR值 CR value	一次性检验结果 Test results
努责Valsalva's maneuver	1.775	35.5	5	0	1.12	0	通过
摸舔产道Touching and licking the birth canal	1.242	24.8
趴卧/躺卧Lying down	1.358	27.2
身体辗转Body rolls	0.525	10.5
翻滚Tumbling	0.100	2.0

组别 Groups	年份 Certain year	孕猴/只 Pregnant monkeys n	怀胎数/只 Pregnancies n	助产/只 Midwifery n	存活仔猴/只 Surviving monkeys n	活产率/% Live birth rate/%	平均活产率/% Average live birth rate/%
监控 Control	2017	36	47	20	36	76.7	87.1^*
	2018	31	37	28	34	91.9
	2019	54	57	28	49	86.0
	2020	64	72	17	62	86.1
	2021	53	58	15	55	94.8
未监控 Unmonitored	2011	14	15	/	8	53.3	63.5
	2013	29	40	/	15	37.5
	2014	33	35	/	28	80.0
	2015	16	19	/	12	63.2
	2016	23	24	/	20	83.3