Study on Pathological Changes and Related Mechanism Induced by Brain Injection of Serum in Rats

doi:10.3969/j.issn.1674-5817.2018.06.002

Abstract

Abstract: Objective To investigate the pathological changes and related mechanism induced by brain injection of serum in rats. Methods Injection of fetal bovine serum (FBS) into rat brain. The control group injected the same volume normal saline, after 48h contrast two groups survival rate, behavioral change, the measurement of brain tissue water content changes, RT-PCR detection of aquaporin-4(AQP-4) mRNA expression ; The expression of AQP-4 protein was detected by Western blot. The expression of AQP-4 mRNA was detected in serum/serum-free culture of rat astrocytes, RT-PCR, and the expression of AQP-4 protein was detected by Western blot. Results The survival rate and behavioral score of the experimental group were significantly improved compared with the control group. The edema of brain tissue around the injection site of the experimental group was shown under electron microscope, the expression of AQP-4 protein and mRNA in the experimental group was significantly higher than that in the control group (P<0.01), the expression of AQP-4 protein and mRNA in the injection site of the experimental group were not significantly different from that of the control group (P<0.05); Compared with serum-free culture group, the expression of AQP-4 protein in astrocytes of rat with serum and mRNA were significantly decreased (P<0.05). Conclusion In vitro and in vivo, it is shown that the decrease of AQP-4 expression in brain tissue, especially astrocytes may be an important molecular mechanism of secondary cerebral edema after intracerebral hemorrhage (ICH), and provide a new therapeutic target for the treatment of cerebral edema after ICH in the future.

Key words: Intracerebral hemorrhage (ICH), Aquaporin-4(AQP-4), Animal models, Cell models

CLC Number:

Q95-33

ZHAO Zhi-jing, DIWU Fei-hu, DENG Yi-heng, YANG Li-sun. Study on Pathological Changes and Related Mechanism Induced by Brain Injection of Serum in Rats[J]. Laboratory Animal and Comparative Medicine, 2018, 38(6): 412-416.

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