Effects of High-fat Diet and Treadmill Exercise on AMP-activated Protein Kinase (AMPK)/Acetyl-CoA Carboxylase (ACC) Signaling Pathway and Fatty Acid Translocase CD36 Protein Content in Rat Gastrocnemius Muscle

doi:10.3969/j.issn.1674-5817.2015.06.003

Abstract

Abstract: Objectives To investigate the effects of high-fat diet and 8 weeks aerobic endurance exercise (treadmill exercise) on AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) signaling pathway and fatty acid translocase CD36 protein content in rat gastrocnemius muscles. Methods A rat model of nutritional obesity was established by feeding rats a high-fat diet, and then the obese rats were randomly divided into obese control group (OC group) and obese exercise group (OE group); In addition, a normal diet control group (NC group) and a normal diet exercise group (NE group) were set up. The exercise groups (OE and NE groups) were arranged by aerobic endurance exercise for 8 weeks. After the experiment, the levels of AMPKa, p-AMPKa, ACC, p-ACC and membrane protein CD36 in all groups were detected by Western blotting in gastrocnemius muscles. Results 1) Compared with the NC group, p-AMPKa protein levels had a significant increase in the NE group (P<0.01). p-AMPKa protein levels in the OE group were significantly higher than that in the OC group (P<0.01). p-AMPKa protein levels in the OC group were significantly lower than that in the NC group (P<0.01). 2) Compared with the NC group, p-ACC protein level in the OC group were significantly decreased (P<0.01). p-ACC protein levels had a significant increase in the OE group as compared with the OC group (P<0.01). 3) Compared between the NE group and NC group, the OE group and OC group, and the OC group and NC group, the content of membrane protein CD36 showed no significant change (P>0.05). Conclusion 1) The treadmill exercise could improve AMPK/ACC signaling pathway. 2) The impacts of exercise on p-ACC levels were different in rats with different body mass. 3) The membrane protein CD36 content in the rat gastrocnemius muscles did not significantly change whether AMPK/ACC signaling pathway was activated or inhibited.

Key words: Treadmill exercise, High-fat diet, Obesity, AMP-activated protein kinase(AMPK), Acetyl-CoA carboxylase (ACC), Fatty acid translocase CD36

CLC Number:

Q95-33

ZHANG Yun-li, LOU Shu-jie. Effects of High-fat Diet and Treadmill Exercise on AMP-activated Protein Kinase (AMPK)/Acetyl-CoA Carboxylase (ACC) Signaling Pathway and Fatty Acid Translocase CD36 Protein Content in Rat Gastrocnemius Muscle[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 441-447.

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