Expression of Pulmonary Surfactant-associated Protein A and CT Imaging in Lung Re-expansion Minipig after Atelectasis

doi:10.3969/j.issn.1674-5817.2015.06.002

Abstract

Abstract: Objectives To investigate the expression of Pulmonary surfactant-associated protein A(SPA) and the CT imaging in lung re-expansion after atelectasis. Methods The minipig model of lung re-expansion after atelectasis was established. Male China Experimental Miniature Pig-I were randomly divided into three-days atelectasis group (3 d), one-week atelectasis group (1 week) and two-weeks atelectasis group (2 weeks), respectively (n=6). Pigs were euthanized after two weeks of lung re-expansion. The expression of SPA of experimental lung tissue was detected by immunohistochemistry. At specified time point, CT value and imaging changes were measured. Results The expression of SPA was lower in 2 weeks group than that in the other two groups (P<0.01). The CT value was lower in 3 d group than that in the other two groups (P<0.01), and the degree of lung re-expansion was the highest level in 3 d group among three groups. Conclusions The SPA expression of experimental lung tissue was decreased with atelectasis prolonged. The CT imaging indicated that improvement of lung re-expansion was dependent on the time of the atelectasis and the preferable opportunity of atelectasis cure was within one week.

Key words: Atelectasis, Re-expansion, Pulmonary surfactant-associated protein A(SPA), CT value

CLC Number:

Q95-33

TU Jun-wei, WANG Sai-bin, SHENG Yi-jun, CHEN Hui-jun, TIAN Jiang-hua, SHENG Lin, YING Ming-liang, Hu Bin, HE Zhong-ping. Expression of Pulmonary Surfactant-associated Protein A and CT Imaging in Lung Re-expansion Minipig after Atelectasis[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 436-514.

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